Q8RFN7 (PGK_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	FN0654

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fusobacteria › Fusobacteriia › Fusobacteriales › Fusobacteriaceae › Fusobacterium › Fusobacterium nucleatum › Fusobacterium nucleatum subsp. nucleatum

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Phosphoglycerate kinase HAMAP-Rule MF_00145		PRO_0000145945
Regions
	Nucleotide binding	354 – 357	4	ATP By similarity
	Region	20 – 22	3	Substrate binding By similarity
	Region	58 – 61	4	Substrate binding By similarity
Sites
	Binding site	35	1	Substrate By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	208	1	ATP By similarity
	Binding site	296	1	ATP; via carbonyl oxygen By similarity
	Binding site	327	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RFN7 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 58D6903772ACEEEB	FASTA	398	42,835
	10 20 30 40 50 60 MKKIITDLDL NNKKVLMRVD FNVPMKDGKI TDENRIVQAL PTIKYVLEHN AKLILFSHLG 70 80 90 100 110 120 KVKIEEDKAT KSLKAVAEKL SELLGKNVTF IPETRGEKLE SAINNLKSGE VLMFENTRFE 130 140 150 160 170 180 DLDGKKESKN DSELGKYWAS LGDVFVNDAF GTAHRAHASN VGIAENIGNG NSAVGFLVEK 190 200 210 220 230 240 ELKFIGEAVN NPKRPLIAIL GGAKVSDKIG VIENLLTKAD KILIGGAMMF TFLKAEGKNI 250 260 270 280 290 300 GTSLVEDDKL DLAKDLLAKS NGKIVLPVDT VIASEFKNDI EFSTVDVDNI PNNKMGLDIG 310 320 330 340 350 360 EKTVTLFDSY IKTAKTVVWN GPMGVFEMSN FAKGTIGVCE SIANLTDAVT IIGGGDSAAA 370 380 390 AISLGYADKF THISTGGGAS LEFLEGKVLP GVEAISNK

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL94850.1.
RefSeq	NP_603551.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RFN7.
SMR	Q8RFN7. Positions 5-61.
ModBase	Search...
Protein-protein interaction databases
STRING	190304.FN0654.
Proteomic databases
PRIDE	Q8RFN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL94850; AAL94850; FN0654.
GeneID	993211.
KEGG	fnu:FN0654.
PATRIC	21951073. VBIFusNuc122357_1219.
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HOG000227108.
KO	K00927.
OMA	KFGLADK.
ProtClustDB	PRK00073.
Enzyme and pathway databases
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
HAMAP	MF_00145. Phosphoglyc_kinase.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_FUSNN

Accession

Primary (citable) accession number: Q8RFN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program