Q8RFG1 (HUTI_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	FN0740

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Imidazolonepropionase HAMAP MF_00372		PRO_0000160948
Sites
	Metal binding	79	1	Zinc or iron By similarity
	Metal binding	81	1	Zinc or iron By similarity
	Metal binding	248	1	Zinc or iron By similarity
	Metal binding	322	1	Zinc or iron By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RFG1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F14316699227C27D	FASTA	413	45,367
	10 20 30 40 50 60 MQADLVLYNI GQLVTSRELD KTKKMDNIEV IENNGYIVIE KDKIVAVGSG EVLKEYLTPA 70 80 90 100 110 120 TEMVDLSGKL VTPGLIDSHT HLVHGGSREN EFAMKIAGVP YLEILEKGGG ILSTLKSTRN 130 140 150 160 170 180 ASEQELIEKT LKSLRHMLEL GVTTVEAKSG YGLNLEDELK QLEVTKILGY LQPVTLVSTF 190 200 210 220 230 240 MAAHATPPEY KDNKEGYVQE VIKMLPIVKE RNLAEFCDIF CEDKVFSVDE SRRILTVAKE 250 260 270 280 290 300 LGYKLKIHAD EIVSLGGVEL AAELGATSAE HLMKITDSGI NALANSNVIA DLLPATSFNL 310 320 330 340 350 360 MEHYAPARKM IEAGIQIALS TDYNPGSCPS ENLQFVMQIG AAHLKMTPKE VFKSVTINAA 370 380 390 400 410 KAIDKQDTIG SIEVGKKADI TVFDAPSMAY FLYHFGINHT DSVYKNGKLV FKK

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL94936.1.
RefSeq	NP_603637.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RFG1.
SMR	Q8RFG1. Positions 2-413.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	991822.
GenomeReviews	Gene locus FN0740 in contig AE009951_GR.
KEGG	fnu:FN0740.
NMPDR	fig|190304.1.peg.1316.
PATRIC	21951241. VBIFusNuc122357_1303.
Phylogenomic databases
HOGENOM	HBG686142.
OMA	MNMACTL.
PhylomeDB	Q8RFG1.
ProtClustDB	PRK09356.
Enzyme and pathway databases
BioCyc	FNUC190304:FN0740-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_FUSNN

Accession

Primary (citable) accession number: Q8RFG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program