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Q8RF62 (GLGB_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:FN0856
OrganismFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]
Taxonomic identifier190304 [NCBI]
Taxonomic lineageBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6116111,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188708

Sites

Active site3021Nucleophile By similarity
Active site3431Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8RF62 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: F022C62F303CC741

FASTA61172,562
        10         20         30         40         50         60 
MILMSGQMEQ YLFHRGEFRQ AYEYFGAHPT RSSTIFRIWA PSAKSVAVVG DFNDWRAREE 

        70         80         90        100        110        120 
DYCHKLTNEG IWEVEIKKIK KGNLYKYQIE TSWGEKILKS DPYAFYSELR PQTASIVNGK 

       130        140        150        160        170        180 
PKFRWADKRW LNNREIGYAK PINIYEVHLG SWKKKEDGTY YNYKEIAELL VEYMLEMNYT 

       190        200        210        220        230        240 
HIEIMPIIEY PFDGSWGYQG TGYYSVTSRY GTPDDFMYFV NYFHKNNLGV ILDWVPGHFC 

       250        260        270        280        290        300 
KDSHGLYRFD GSACYEYEDP SLGENEWGSA NFNVSRNEVR SFLLSNLYFW IKEFHIDGIR 

       310        320        330        340        350        360 
MDAVSNMLYY KDGLSENKHS VEFLQYLNQS LHEEYPDVML IAEDSSAWPL VTKYQADGGL 

       370        380        390        400        410        420 
GFDFKWNMGW MNDTLKYMEQ DPFFRKSHHG KLTFSFMYAF SENFILPLSH DEIVHGKNSI 

       430        440        450        460        470        480 
LNKMPGYYED KLAHVKNLYS YQMAHPGKKL NFMGNEFVQG LEWRYYEQLE WQLLKDNKGS 

       490        500        510        520        530        540 
QDIQKYVKAL NKLYLEEEAL WYDGQDGFEW IEHENINENM LIFLRKTPNM EDFIIAVFNF 

       550        560        570        580        590        600 
SGKDHEIYPL GVPLEDGEYE VILDSNEKKF GGSYQGRKRK YKSIKKSWNY REQYIEIKIA 

       610 
KNSAVFLKYK K 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009951 Genomic DNA. Translation: AAL95052.1.
RefSeqNP_603753.1. NC_003454.1.

3D structure databases

ProteinModelPortalQ8RF62.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190304.FN0856.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL95052; AAL95052; FN0856.
GeneID991540.
KEGGfnu:FN0856.
PATRIC21951469. VBIFusNuc122357_1417.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_FUSNN
AccessionPrimary (citable) accession number: Q8RF62
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries