Reviewed, UniProtKB/Swiss-Prot Q8REV7 (PUR2_FUSNN)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	FN0981

Organism

Fusobacterium nucleatum subsp. nucleatum [Complete proteome] [HAMAP]

Taxonomic identifier

76856 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151451
Regions
	Domain	107 – 313	207	ATP-grasp
	Nucleotide binding	133 – 194	62	ATP By similarity
Sites
	Metal binding	283	1	Magnesium or manganese By similarity
	Metal binding	285	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8REV7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 297AB4260A3D9D5E	FASTA	426	47,319
	10 20 30 40 50 60 MKVLIVGSGG REHAIAWKIS QNSKVDKIFA ASGNAYNKVI KNCENINLKT SDDILNFAIK 70 80 90 100 110 120 EKVDLTIVGS EELLVDGIVD KFQENNLTIF GPNKEAAMLE GSKAFAKDFM QKYGVKTAKY 130 140 150 160 170 180 QSFIDKEKAI KYLDKISYPV VIKASGLAAG KGVVIAQNRK EAEETLNDMM TNKVFAAAGD 190 200 210 220 230 240 TVVIEEFLDG VEISVLSITD SEVIIPFISA KDHKKISEKE TGLNTGGMGV IAPNPYYTKT 250 260 270 280 290 300 IEEKFIQNIL NPTLKGIKAE KMNFVGIIFF GLMVANGEVY LLEYNMRMGD PETQAVLPLM 310 320 330 340 350 360 KSDFLNVINS ALNKDLKNIK IDWEDKSACC VVMAAGGYPV KYEKGNFISG LEKFDSNKSD 370 380 390 400 410 420 NKIFFAGVKE ENDKFYTNGG RVLNVVSIKD SLEKAIEEAY KNVKEISFKD NYYRKDIGTL YVPVKY

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009951 Genomic DNA. Translation: AAL95177.1.
RefSeq	NP_603878.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	991454.
GenomeReviews	Gene locus FN0981 in contig AE009951_GR.
KEGG	fnu:FN0981.
NMPDR	fig|190304.1.peg.1557.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8REV7.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	FNUC190304:FN0981-MON.
BRENDA	6.3.4.13. 289963.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_FUSNN

Accession

Primary (citable) accession number: Q8REV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)