Q8REV3 (PUR5_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	FN0986

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fusobacteria › Fusobacteriia › Fusobacteriales › Fusobacteriaceae › Fusobacterium › Fusobacterium nucleatum › Fusobacterium nucleatum subsp. nucleatum

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000148213

Sequences

	Sequence		Length	Mass (Da)
	Q8REV3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: FF6C6C7B048C9572	FASTA	339	37,126
	10 20 30 40 50 60 MINSYKDSGV DKEEGYKAVE LMKKNVLKTH NKSVLTNLGS FGAMYELGQY KNPVLISGTD 70 80 90 100 110 120 GVGTKLEIAM KQKKYDTVGI DCVAMCVNDV LCHGAKPLFF LDYLACGKLD AEVAAQLVSG 130 140 150 160 170 180 VTEGCLQSYA ALVGGETAEM PGFYKEGDYD IAGFCVGIVE KEDLIDGSKV KEGNKIIAVA 190 200 210 220 230 240 SSGFHSNGYS LVRKVFTDYN EKIFLKEYGE NVTMGDVLLT PTKIYVKPIL KVLEKFNVNG 250 260 270 280 290 300 MAHITGGGLF ENLPRCMGKN LSPVVFKDKV RVPEIFKLIA ERSKIKEEEL FGTFNMGVGF 310 320 330 TLVVEEKDVE TIIELLTSLG ETAYEIGHIE KGDHSLCLK

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL95182.1.
RefSeq	NP_603883.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8REV3.
ModBase	Search...
Protein-protein interaction databases
STRING	190304.FN0986.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL95182; AAL95182; FN0986.
GeneID	991451.
KEGG	fnu:FN0986.
PATRIC	21951737. VBIFusNuc122357_1551.
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229091.
KO	K01933.
OMA	IDMIAMN.
ProtClustDB	PRK05385.
Enzyme and pathway databases
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_FUSNN

Accession

Primary (citable) accession number: Q8REV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program