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Q8REU0 (BIOB_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Biotin synthase HAMAP-Rule MF_01694
PRO_0000381399

Sites

Metal binding1011Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1051Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1081Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1451Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1801Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2401Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3101Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8REU0 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8DEC992669EA072C

FASTA36040,450
        10         20         30         40         50         60 
MLKEKNSAGG GKFNFFNFLK EKDNNQAEPS NVKEFISYLK NKIINEKYEI TREEAIFLSQ 

        70         80         90        100        110        120 
IPNNDMETLN ILFNAADQIR EVFCGKYFDL CTIINAKSGK CSENCKYCAQ SVHFKTGTDV 

       130        140        150        160        170        180 
YGLISKELAL YEAKRNENEG AHRFSLVTSG RGLNGNEKEL DKLVEIYKYI GEHTSKLELC 

       190        200        210        220        230        240 
ASHGICTKEA LQKLVDAGVL TYHHNLESSR RFYPNVCTSH SYDDRINTIK NAKAVGLDVC 

       250        260        270        280        290        300 
SGGIFGLGET IEDRIDMALD LRALEIHSVP INVLTPIPGT PFENNEEVNP FEILKTISIY 

       310        320        330        340        350        360 
RFIMPKSFLR YCGGRIKLGE HAKTGLRCGI NSALTGNFLT TTGTTIETDK KMIKELGYEI 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009951 Genomic DNA. Translation: AAL95196.1.
RefSeqNP_603897.1. NC_003454.1.

3D structure databases

ProteinModelPortalQ8REU0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190304.FN1000.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL95196; AAL95196; FN1000.
GeneID993063.
KEGGfnu:FN1000.
PATRIC21951765. VBIFusNuc122357_1565.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_FUSNN
AccessionPrimary (citable) accession number: Q8REU0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2002
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways