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Q8REU0

- BIOB_FUSNN

UniProt

Q8REU0 - BIOB_FUSNN

Protein

Biotin synthase

Gene

bioB

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi145 – 1451Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi180 – 1801Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi240 – 2401Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi310 – 3101Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:FN1000
    OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
    Taxonomic identifieri190304 [NCBI]
    Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
    ProteomesiUP000002521: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Biotin synthasePRO_0000381399Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi190304.FN1000.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8REU0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8REU0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKEKNSAGG GKFNFFNFLK EKDNNQAEPS NVKEFISYLK NKIINEKYEI    50
    TREEAIFLSQ IPNNDMETLN ILFNAADQIR EVFCGKYFDL CTIINAKSGK 100
    CSENCKYCAQ SVHFKTGTDV YGLISKELAL YEAKRNENEG AHRFSLVTSG 150
    RGLNGNEKEL DKLVEIYKYI GEHTSKLELC ASHGICTKEA LQKLVDAGVL 200
    TYHHNLESSR RFYPNVCTSH SYDDRINTIK NAKAVGLDVC SGGIFGLGET 250
    IEDRIDMALD LRALEIHSVP INVLTPIPGT PFENNEEVNP FEILKTISIY 300
    RFIMPKSFLR YCGGRIKLGE HAKTGLRCGI NSALTGNFLT TTGTTIETDK 350
    KMIKELGYEI 360
    Length:360
    Mass (Da):40,450
    Last modified:June 1, 2002 - v1
    Checksum:i8DEC992669EA072C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009951 Genomic DNA. Translation: AAL95196.1.
    RefSeqiNP_603897.1. NC_003454.1.

    Genome annotation databases

    EnsemblBacteriaiAAL95196; AAL95196; FN1000.
    GeneIDi993063.
    KEGGifnu:FN1000.
    PATRICi21951765. VBIFusNuc122357_1565.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009951 Genomic DNA. Translation: AAL95196.1 .
    RefSeqi NP_603897.1. NC_003454.1.

    3D structure databases

    ProteinModelPortali Q8REU0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190304.FN1000.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL95196 ; AAL95196 ; FN1000 .
    GeneIDi 993063.
    KEGGi fnu:FN1000.
    PATRICi 21951765. VBIFusNuc122357_1565.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

    Entry informationi

    Entry nameiBIOB_FUSNN
    AccessioniPrimary (citable) accession number: Q8REU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3