Q8RET9 (BIOD_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	FN1001

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187966
Regions
	Nucleotide binding	114 – 117	4	ATP By similarity
	Nucleotide binding	175 – 176	2	ATP By similarity
Sites
	Metal binding	14	1	Magnesium 1 By similarity
	Metal binding	18	1	Magnesium 2 By similarity
	Metal binding	54	1	Magnesium 2 By similarity
	Metal binding	114	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	54	1	ATP By similarity
	Binding site	219	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RET9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D9D16A17E1A09DB6	FASTA	219	25,149
	10 20 30 40 50 60 MKFKDFFVIG TDTDVGKTYV STLLYKALKK YNFQYYKPIQ SGCFLKDGKL TAPDVDFLTK 70 80 90 100 110 120 FVGIDYDDSM VTYTLKEEVS PHLASEMEGI RIEIENVKRH YEDLKKKHSN ILVEGAGGLY 130 140 150 160 170 180 VPLIRDIFYI YDLIKLFNLP VVLVCGTKVG AINHTMLTLN ALDTMGIKLH GLVFNNYRGQ 190 200 210 FFEDDNIKVV LALSKIKNYL IIKNGQKEIS DKEIEKFFN

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL95197.1.
RefSeq	NP_603898.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RET9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	991443.
GenomeReviews	Gene locus FN1001 in contig AE009951_GR.
KEGG	fnu:FN1001.
NMPDR	fig|190304.1.peg.1577.
PATRIC	21951767. VBIFusNuc122357_1566.
Phylogenomic databases
OMA	ANCVDPN.
PhylomeDB	Q8RET9.
Enzyme and pathway databases
BioCyc	FNUC190304:FN1001-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_FUSNN

Accession

Primary (citable) accession number: Q8RET9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program