Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79Substrate1
Binding sitei93Substrate; via amide nitrogen and carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:FN1028
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
Proteomesi
  • UP000002521 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828631 – 146Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST146

Interactioni

Protein-protein interaction databases

STRINGi190304.FN1028.

Structurei

3D structure databases

ProteinModelPortaliQ8RER7.
SMRiQ8RER7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni66 – 68Substrate binding3
Regioni83 – 85Substrate binding3

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

eggNOGiENOG4108Z1K. Bacteria.
COG0756. LUCA.
HOGENOMiHOG000028968.
InParanoidiQ8RER7.
KOiK01520.
OMAiGVILINH.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiView protein in InterPro
IPR029054. dUTPase-like.
IPR036157. dUTPase-like_sf.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
PfamiView protein in Pfam
PF00692. dUTPase. 1 hit.
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RER7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVQVKVIR EEGVELPKYE TEGSAGMDVR ANIKESITLK SLERILVPTG
60 70 80 90 100
LKVAIPEGYE IQVRPRSGLA IKHGITMLNT PGTVDSDYRG ELKVIVVNLS
110 120 130 140
NEAYTIEPNE RIGQFVLNKI EQIEFVEVEE LDSTERGESG FGHTGK
Length:146
Mass (Da):16,226
Last modified:June 1, 2002 - v1
Checksum:iF615D978CE30298B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL95224.1.
RefSeqiNP_603925.1. NC_003454.1.
WP_011016845.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL95224; AAL95224; FN1028.
GeneIDi991429.
KEGGifnu:FN1028.
PATRICifig|190304.8.peg.1592.

Similar proteinsi

Entry informationi

Entry nameiDUT_FUSNN
AccessioniPrimary (citable) accession number: Q8RER7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: October 25, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families