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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:FN1070
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
Proteomesi
  • UP000002521 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117244Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190304.FN1070.

Structurei

3D structure databases

ProteinModelPortaliQ8REM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
InParanoidiQ8REM9.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.50.50.60. 5 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8REM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKEVIVVGA GLAGSEAAYQ LAKRGIKVKL YEMKAKKKTP AHSKDYFSEL
60 70 80 90 100
VCSNSLGSDS LENASGLMKE ELRILGSLLI EVADKNRVPA GQALAVDRDG
110 120 130 140 150
FSEEVTKILK NTKNIEIIEE EFTEFPNDKI VIIASGPLTS DKLFQKISEI
160 170 180 190 200
TGEESLYFYD AAAPIVTFES IDMNKAYFQS RYGKGDGEYI NCPMNKEEYY
210 220 230 240 250
NFYNELIKAE RAELKNFEKE KLFDACMPIE KIAMSGEKTM TFGPLKPKGL
260 270 280 290 300
INPKTEKMDY AVVQLRQDDK EGKLYNIVGF QTNLKFGEQK RVFSMIPGLE
310 320 330 340 350
NAEFIRYGVM HRNTFINSTK LLDKTLKLKN KDNIYFAGQI TGGEGYVTAI
360 370 380 390 400
ATGMYVAMNV ANRLENKEEF ILEDISEIGA IVNYITEEKK KFQPMGANFG
410 420 430
IIRSLDENIR DKKEKYRKLS ERAIEYLKNS IKGV
Length:434
Mass (Da):49,109
Last modified:June 1, 2002 - v1
Checksum:i4492022F5CF6BBB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL95266.1.
RefSeqiNP_603967.1. NC_003454.1.
WP_011016878.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL95266; AAL95266; FN1070.
GeneIDi991346.
KEGGifnu:FN1070.
PATRICi21951905. VBIFusNuc122357_1635.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL95266.1.
RefSeqiNP_603967.1. NC_003454.1.
WP_011016878.1. NC_003454.1.

3D structure databases

ProteinModelPortaliQ8REM9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190304.FN1070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL95266; AAL95266; FN1070.
GeneIDi991346.
KEGGifnu:FN1070.
PATRICi21951905. VBIFusNuc122357_1635.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
InParanoidiQ8REM9.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.50.50.60. 5 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_FUSNN
AccessioniPrimary (citable) accession number: Q8REM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.