Reviewed, UniProtKB/Swiss-Prot Q8REM9 (TRMFO_FUSNN)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	FN1070

Organism

Fusobacterium nucleatum subsp. nucleatum [Complete proteome] [HAMAP]

Taxonomic identifier

76856 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000117244
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8REM9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 4492022F5CF6BBB6	FASTA	434	49,109
	10 20 30 40 50 60 MEKEVIVVGA GLAGSEAAYQ LAKRGIKVKL YEMKAKKKTP AHSKDYFSEL VCSNSLGSDS 70 80 90 100 110 120 LENASGLMKE ELRILGSLLI EVADKNRVPA GQALAVDRDG FSEEVTKILK NTKNIEIIEE 130 140 150 160 170 180 EFTEFPNDKI VIIASGPLTS DKLFQKISEI TGEESLYFYD AAAPIVTFES IDMNKAYFQS 190 200 210 220 230 240 RYGKGDGEYI NCPMNKEEYY NFYNELIKAE RAELKNFEKE KLFDACMPIE KIAMSGEKTM 250 260 270 280 290 300 TFGPLKPKGL INPKTEKMDY AVVQLRQDDK EGKLYNIVGF QTNLKFGEQK RVFSMIPGLE 310 320 330 340 350 360 NAEFIRYGVM HRNTFINSTK LLDKTLKLKN KDNIYFAGQI TGGEGYVTAI ATGMYVAMNV 370 380 390 400 410 420 ANRLENKEEF ILEDISEIGA IVNYITEEKK KFQPMGANFG IIRSLDENIR DKKEKYRKLS 430 ERAIEYLKNS IKGV

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009951 Genomic DNA. Translation: AAL95266.1.
RefSeq	NP_603967.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	991346.
GenomeReviews	Gene locus FN1070 in contig AE009951_GR.
KEGG	fnu:FN1070.
NMPDR	fig|190304.1.peg.1646.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8REM9.
OMA	Q8REM9. MKPVGLT.
Enzyme and pathway databases
BioCyc	FNUC190304:FN1070-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_FUSNN

Accession

Primary (citable) accession number: Q8REM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)