Reviewed, UniProtKB/Swiss-Prot Q8REA7 (NADE_FUSNN)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	FN1202

Organism

Fusobacterium nucleatum subsp. nucleatum [Complete proteome] [HAMAP]

Taxonomic identifier

76856 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152171
Regions
	Nucleotide binding	34 – 41	8	ATP By similarity
Sites
	Active site	36	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8REA7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 760CEC7C9A7D517B	FASTA	258	29,493
	10 20 30 40 50 60 MNKLDLNLKE VHNELVEFLR ENFKKAGFSK AVLGLSGGID SALVAYLLRD ALGKENVLAI 70 80 90 100 110 120 MMPYKSSNPD SLNHAKLVVE DLKINSKTIE ITDMIDAYFK NEKEATSLRM GNKMARERMS 130 140 150 160 170 180 ILFDYSSKEN ALVVGTSNKT EIYLGYSTQF GDAACALNPI GDLYKTNIWD LSRYLKIPNE 190 200 210 220 230 240 LIEKKPSADL WEGQTDEQEM GLTYKEADQV MYRLLEENKT VEEVLAEGFN KDLVDNIVRR 250 MNRSEYKRRM PLIAKIKR

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009951 Genomic DNA. Translation: AAL95398.1.
RefSeq	NP_604099.1.
3D structure databases
HSSP	HSSP built from PDB template 1IH8 based on UniProtKB P08164.
ModBase	Search...
Genome annotation databases
GeneID	991988.
GenomeReviews	Gene locus FN1202 in contig AE009951_GR.
KEGG	fnu:FN1202.
NMPDR	fig|190304.1.peg.1778.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8REA7.
OMA	LYAFAQK.
Enzyme and pathway databases
BioCyc	FNUC190304:FN1202-MON.
BRENDA	6.3.1.5. 289963.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_FUSNN

Accession

Primary (citable) accession number: Q8REA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)