Q8RE91 (KDSA_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	FN1224

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.
Sequence caution	The sequence AAL95420.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000187128

Sequences

	Sequence		Length	Mass (Da)
	Q8RE91 [UniParc]. Last modified October 24, 2003. Version 2. Checksum: AF0B460EA5309AE8	FASTA	278	30,937
	10 20 30 40 50 60 MLINDVNKVK VGNIVFGGKK RFVLIAGPCV MESQELMDEV AGGIKEICDR LGIEYIFKAS 70 80 90 100 110 120 FDKANRSSIY SYRGPGLEEG MKMLTKIKEK FNVPVITDVH EAWQCKEVAK VADILQIPAF 130 140 150 160 170 180 LCRQTDLLIA AAETGKAVNI KKGQFLAPWD MKNIVVKMEE SRNKNIMLCE RGSTFGYNNM 190 200 210 220 230 240 VVDMRSLLEM RKFNYPVIFD VTHSVQKPGG LGTATSGDRE YVYPLLRAGL AIGVDAIFAE 250 260 270 VHPNPAEAKS DGPNMLYLKD LEEILKIAIE IDKIVKGV

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL95420.1. Different initiation.
RefSeq	NP_604121.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RE91.
SMR	Q8RE91. Positions 20-276.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	992017.
GenomeReviews	Gene locus FN1224 in contig AE009951_GR.
KEGG	fnu:FN1224.
NMPDR	fig|190304.1.peg.1800.
PATRIC	21952209. VBIFusNuc122357_1787.
Phylogenomic databases
HOGENOM	HBG575990.
OMA	HEPHQAA.
PhylomeDB	Q8RE91.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	FNUC190304:FN1224-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_FUSNN

Accession

Primary (citable) accession number: Q8RE91

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	October 24, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program