Q8RDZ8 (SYE_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	FN1340

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAL95536.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119565
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00022_B
	Motif	256 – 260	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	259	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RDZ8 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: B2A87FEDC9BEDBBD	FASTA	510	58,753
	10 20 30 40 50 60 MCVDCKKRVR TRVAPSPTGD PHVGTAYIAL FNIAFAHVND GDFILRIEDT DRNRYTEGSE 70 80 90 100 110 120 QMIFDALKWL DLDYSEGPDV GGDYGPYRQS ERFDLYGKYA KELVEKGGAY YCFCDHERLE 130 140 150 160 170 180 NLRERQKAMG LPPGYDGHCR SLSKEEIEEK LKAGVPYVIR LKMPYEGETV IHDRLRGDVV 190 200 210 220 230 240 FENSKIDDQV LLKADGYPTY HLANIVDDHL MGITHVIRAE EWIPSTPKHI QLYKAFGWEA 250 260 270 280 290 300 PEFIHMPLLR NDDRSKISKR KNPVSLIWYK EEGYLKEGLV NFLGLMGYSY GDGQEIFTLQ 310 320 330 340 350 360 EFKDNFNIDK VTLGGPVFDL VKLGWVNNQH MKMKDLGELT RLTIPFFVNE GYLTNENVSE 370 380 390 400 410 420 KEFETLKKVV GIEREGAKTL QELAKNSKFF FVDEFSLPEL REDMDKKERK SVERLLNSLK 430 440 450 460 470 480 DEIGLKSIKL FIEKLEKWNG NEFTAEQAKD LLHSLLDDLQ EGPGKIFMPI RAVLTGESKG 490 500 510 ADLYNILYVI GKERALKRIK NIVKKYNIGI

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL95536.1. Different initiation.
RefSeq	NP_604237.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8RDZ8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	992212.
GenomeReviews	Gene locus FN1340 in contig AE009951_GR.
KEGG	fnu:FN1340.
NMPDR	fig|190304.1.peg.1916.
PATRIC	21952441. VBIFusNuc122357_1903.
Phylogenomic databases
HOGENOM	HBG628189.
OMA	WENVRVR.
PhylomeDB	Q8RDZ8.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	FNUC190304:FN1340-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_FUSNN

Accession

Primary (citable) accession number: Q8RDZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program