Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8RDZ8 (SYE_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:FN1340
OrganismFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]
Taxonomic identifier190304 [NCBI]
Taxonomic lineageBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAL95536.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 510510Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000119565

Regions

Motif15 – 2511"HIGH" region HAMAP-Rule MF_00022
Motif256 – 2605"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2591ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8RDZ8 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: B2A87FEDC9BEDBBD

FASTA51058,753
        10         20         30         40         50         60 
MCVDCKKRVR TRVAPSPTGD PHVGTAYIAL FNIAFAHVND GDFILRIEDT DRNRYTEGSE 

        70         80         90        100        110        120 
QMIFDALKWL DLDYSEGPDV GGDYGPYRQS ERFDLYGKYA KELVEKGGAY YCFCDHERLE 

       130        140        150        160        170        180 
NLRERQKAMG LPPGYDGHCR SLSKEEIEEK LKAGVPYVIR LKMPYEGETV IHDRLRGDVV 

       190        200        210        220        230        240 
FENSKIDDQV LLKADGYPTY HLANIVDDHL MGITHVIRAE EWIPSTPKHI QLYKAFGWEA 

       250        260        270        280        290        300 
PEFIHMPLLR NDDRSKISKR KNPVSLIWYK EEGYLKEGLV NFLGLMGYSY GDGQEIFTLQ 

       310        320        330        340        350        360 
EFKDNFNIDK VTLGGPVFDL VKLGWVNNQH MKMKDLGELT RLTIPFFVNE GYLTNENVSE 

       370        380        390        400        410        420 
KEFETLKKVV GIEREGAKTL QELAKNSKFF FVDEFSLPEL REDMDKKERK SVERLLNSLK 

       430        440        450        460        470        480 
DEIGLKSIKL FIEKLEKWNG NEFTAEQAKD LLHSLLDDLQ EGPGKIFMPI RAVLTGESKG 

       490        500        510 
ADLYNILYVI GKERALKRIK NIVKKYNIGI 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009951 Genomic DNA. Translation: AAL95536.1. Different initiation.
RefSeqNP_604237.1. NC_003454.1.

3D structure databases

ProteinModelPortalQ8RDZ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190304.FN1340.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL95536; AAL95536; FN1340.
GeneID992212.
KEGGfnu:FN1340.
PATRIC21952441. VBIFusNuc122357_1903.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAEIFDMEY.
OrthoDBEOG6DRPF7.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_FUSNN
AccessionPrimary (citable) accession number: Q8RDZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries