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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei259 – 2591ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:FN1340
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
ProteomesiUP000002521 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510Glutamate--tRNA ligasePRO_0000119565Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi190304.FN1340.

Structurei

3D structure databases

ProteinModelPortaliQ8RDZ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 2511"HIGH" regionAdd
BLAST
Motifi256 – 2605"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
InParanoidiQ8RDZ8.
KOiK01885.
OMAiNKLTWIN.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RDZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCVDCKKRVR TRVAPSPTGD PHVGTAYIAL FNIAFAHVND GDFILRIEDT
60 70 80 90 100
DRNRYTEGSE QMIFDALKWL DLDYSEGPDV GGDYGPYRQS ERFDLYGKYA
110 120 130 140 150
KELVEKGGAY YCFCDHERLE NLRERQKAMG LPPGYDGHCR SLSKEEIEEK
160 170 180 190 200
LKAGVPYVIR LKMPYEGETV IHDRLRGDVV FENSKIDDQV LLKADGYPTY
210 220 230 240 250
HLANIVDDHL MGITHVIRAE EWIPSTPKHI QLYKAFGWEA PEFIHMPLLR
260 270 280 290 300
NDDRSKISKR KNPVSLIWYK EEGYLKEGLV NFLGLMGYSY GDGQEIFTLQ
310 320 330 340 350
EFKDNFNIDK VTLGGPVFDL VKLGWVNNQH MKMKDLGELT RLTIPFFVNE
360 370 380 390 400
GYLTNENVSE KEFETLKKVV GIEREGAKTL QELAKNSKFF FVDEFSLPEL
410 420 430 440 450
REDMDKKERK SVERLLNSLK DEIGLKSIKL FIEKLEKWNG NEFTAEQAKD
460 470 480 490 500
LLHSLLDDLQ EGPGKIFMPI RAVLTGESKG ADLYNILYVI GKERALKRIK
510
NIVKKYNIGI
Length:510
Mass (Da):58,753
Last modified:April 30, 2003 - v2
Checksum:iB2A87FEDC9BEDBBD
GO

Sequence cautioni

The sequence AAL95536.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL95536.1. Different initiation.
RefSeqiNP_604237.1. NC_003454.1.
WP_011017084.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL95536; AAL95536; FN1340.
GeneIDi992212.
KEGGifnu:FN1340.
PATRICi21952441. VBIFusNuc122357_1903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL95536.1. Different initiation.
RefSeqiNP_604237.1. NC_003454.1.
WP_011017084.1. NC_003454.1.

3D structure databases

ProteinModelPortaliQ8RDZ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190304.FN1340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL95536; AAL95536; FN1340.
GeneIDi992212.
KEGGifnu:FN1340.
PATRICi21952441. VBIFusNuc122357_1903.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
InParanoidiQ8RDZ8.
KOiK01885.
OMAiNKLTWIN.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Entry informationi

Entry nameiSYE_FUSNN
AccessioniPrimary (citable) accession number: Q8RDZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: June 24, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.