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Protein

DNA gyrase subunit A

Gene

GyrA

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei122O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, Isomerase, TopoisomeraseUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:GyrAImported
Synonyms:gyrAUniRule annotation
Ordered Locus Names:TTE0011Imported
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)Imported
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiQ8RDK6.

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi273068.TTE0011.

Structurei

3D structure databases

ProteinModelPortaliQ8RDK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 463TOP4cInterPro annotationAdd BLAST453

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili435 – 476Sequence analysisAdd BLAST42

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi524 – 530GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the topoisomerase GyrA/ParC subunit family.UniRule annotation
Belongs to the type II topoisomerase GyrA/ParC subunit family.SAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
HOGENOMiHOG000076279.
KOiK02469.
OMAiTHHWLLF.
OrthoDBiPOG091H025U.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.90.199.10. 1 hit.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiView protein in InterPro
IPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_rep.
IPR035516. Gyrase/topoIV_suA_C.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
PfamiView protein in Pfam
PF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
SMARTiView protein in SMART
SM00434. TOP4c. 1 hit.
SUPFAMiSSF101904. SSF101904. 1 hit.
SSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RDK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEEMDKVIP VDIEDEMRNS YIDYAMSVIV GRALPDVRDG LKPVHRRILY
60 70 80 90 100
AMNEMGLTPD KPYKKSVAVV GEVLGKYHPH GDAAVYDTLV RLAQDFSMRE
110 120 130 140 150
VLIDGHGNFG SIDGDPPAAM RYTEARLSRI ALEMLTDINK ETVDFVPNFD
160 170 180 190 200
ETLKEPVVLP SRFPNLLVNG SQGIAVGMAT NIPPHNLAEV IDGIVAYIDN
210 220 230 240 250
PYITVDELMK YIKGPDFPTG GIILGRDGIK ETYETGRGKI IVRAKAEIEE
260 270 280 290 300
HKGKTRIVIT EIPYMVLKAK LIEKIAELVH NKQIEGIADL RDESDRNGMK
310 320 330 340 350
IVIELKRDVN PKVILNKLYM HTQMQQTFGA IMLALVDGEP KILNLKQIIE
360 370 380 390 400
KYVDHQADVV TRRTKFDLKK AEERAHILEG LKIALDYIDE VINIIRSSKT
410 420 430 440 450
EAIAKENLMK RFGLSDKQAQ AIVDMRLGRL TGLERQKVED ELKELIEKIK
460 470 480 490 500
ELKEILADER KVLEIIKKEL LEIKNKYASP RKTVIVAKEE ELDFEDLVQL
510 520 530 540 550
EDVVVTMTHY GYIKRMPLDT YKSQKRGGKG ILGISTREDD FVEDIFITTT
560 570 580 590 600
HDRLLFFTNK GKVYSLRTID IPESGRQAKG TAIVNLIQIS QGEKVTAVIP
610 620 630 640 650
LKKSDDIKYV LMCTKKGIIK KTSIEEFKSI KRNGIIAITL DENDELINVK
660 670 680 690 700
LTNGEREVII GTAKGYAIRF NEKDVRPMGR VARGVMAISL REDDEVVEMD
710 720 730 740 750
LVHPDGEVLT VTEKGFGKRT DLEEYRIQGR AGKGIIAMKL SKKTGKLVSI
760 770 780 790 800
RCVQPEDEFM IISANGILIR MKVKDISKMH RDTQGVTLMK LEDGDRVVST

ARIESEE
Length:807
Mass (Da):91,163
Last modified:June 1, 2002 - v1
Checksum:i6650EAA86AD34889
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM23328.1.
RefSeqiWP_011024547.1. NC_003869.1.

Genome annotation databases

EnsemblBacteriaiAAM23328; AAM23328; TTE0011.
KEGGitte:TTE0011.

Similar proteinsi

Entry informationi

Entry nameiQ8RDK6_CALS4
AccessioniPrimary (citable) accession number: Q8RDK6
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: September 27, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported