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Q8RCT6 (SYL_THETN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:TTE0324
OrganismThermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]
Taxonomic identifier273068 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter

Protein attributes

Sequence length819 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 819819Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152107

Regions

Motif42 – 5312"HIGH" region HAMAP-Rule MF_00049
Motif578 – 5825"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5811ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8RCT6 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 7D9CB46702513E83

FASTA81995,501
        10         20         30         40         50         60 
MEDMAYSVEI DRKWQKIWEE TKLYKFNPEN VDKKLYCLEM FPYPSGAKLH IGHWYNYGPT 

        70         80         90        100        110        120 
DSWARMKRMQ GYEVFHPMGF DAFGLPAENY AIKTGIHPYD STMENIRTME RQLREMGVTF 

       130        140        150        160        170        180 
DWDYEIITCL PEYYKWTQWI FLKLYEAGLA YRKKAPVNWC PSCQTVLANE QVIDGKCERC 

       190        200        210        220        230        240 
GTEVTKKELT QWFFKITAYA EELLEKLDDL DWPEKTKMMQ RNWIGKSEGA EIEFKIDGKD 

       250        260        270        280        290        300 
LSFRVFTTRA DTLFGATYVV LAPEHELVDL ITTEEYKEAV EEYKEYAKKQ SEIERLSTEK 

       310        320        330        340        350        360 
EKTGVFTGAY AIHPLTGEKL PIWIADYVLV TYGTGCVMGV PGHDERDYEF ATKYNLPIKR 

       370        380        390        400        410        420 
VIKGVGDVDD SLPFVEYGIL VNSEEFTGMT SEEARVKIVE KLKAEGKAEF KVNYRMRDWL 

       430        440        450        460        470        480 
VSRQRYWGAP IPIIHCERCG IVPVPEEDLP VLLPYDVEFE PTGESPLKKH AGFMNVTCPK 

       490        500        510        520        530        540 
CGGPALRDPD TLDTFVDSSW YYLRYPDNKN DKEPFNKEWI NKMLPVDKYV GGAEHATMHL 

       550        560        570        580        590        600 
LYSRFITKVL RDLGYLNFDE PFLSLVHQGT ILGPDGSRMS KSRGNVISPD DYIKQYGSDV 

       610        620        630        640        650        660 
FRLYLMFGFS YSEGGPWSDE GIKAIARFVN RVERFIEKFI ETRQNPGKTK EEMEDAEKEL 

       670        680        690        700        710        720 
NYVRHYTIKH VTLDADKFEF NTAIARIMEL VNALYKYENE VEVKNMKFYE DVVRDFVKIL 

       730        740        750        760        770        780 
APFAPHFSEE MWERLGYEYS VFNQKWPEWD EKALERDMIE IAIQVNGKVR SKAQVPSNAT 

       790        800        810 
DEELKQIALS DERVKSYLDG KEIKKVIIVK NRLVNIVVN 

« Hide

References

[1]"A complete sequence of the T. tengcongensis genome."
Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. expand/collapse author list , Wang J., Yu J., Yang H.
Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008691 Genomic DNA. Translation: AAM23616.1.
RefSeqNP_622012.1. NC_003869.1.

3D structure databases

ProteinModelPortalQ8RCT6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273068.TTE0324.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM23616; AAM23616; TTE0324.
GeneID996294.
KEGGtte:TTE0324.
PATRIC23895281. VBITheTen82880_0316.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCSUB273068:GJEB-586-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_THETN
AccessionPrimary (citable) accession number: Q8RCT6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries