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Q8RC54 (PUR2_THETN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:TTE0593
OrganismThermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]
Taxonomic identifier273068 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151496

Regions

Domain107 – 313207ATP-grasp
Nucleotide binding133 – 19462ATP By similarity

Sites

Metal binding2831Magnesium or manganese By similarity
Metal binding2851Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8RC54 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: A10603882A594C2D

FASTA41746,436
        10         20         30         40         50         60 
MKVLVVGGGG REHAIVHKLS QSDRVEKIYC APGNAGIGQL AECVNISVEE IEKLKEFALQ 

        70         80         90        100        110        120 
NKIDITIVGP ELPLVEGIVD EFERTGLKIF GPSKKAAMIE GSKYFAKQIM AKYEIPTGRF 

       130        140        150        160        170        180 
KAFDRYQEAL KFLKETWYPV VIKADGLAQG KGVFIVRDFI EAKEVLDLMM KKRVFGPSGD 

       190        200        210        220        230        240 
IVIVEEMLYG KEASIFAFVD GENVLTMMTA MDYKKVYEKD EGPNTGGMGS IAPNPHIDKK 

       250        260        270        280        290        300 
TLNEIEEKIL KPVVYALKKE GIVYKGVLYA GLMLTKEGPK VLEFNARFGD PETQAILPLL 

       310        320        330        340        350        360 
KTDFLEIIEA TLEGKLKNLK LEWEDKKAVC VIAASKGYPG EYKKGFEIRG LEEVKEAFVY 

       370        380        390        400        410 
HAGTSFKDGK IVTSGGRVFG IVALGDSYKE AREIAYREIE KISFEGIYYR KDIAAGY 

« Hide

References

[1]"A complete sequence of the T. tengcongensis genome."
Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. expand/collapse author list , Wang J., Yu J., Yang H.
Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008691 Genomic DNA. Translation: AAM23863.1.
RefSeqNP_622259.1. NC_003869.1.

3D structure databases

ProteinModelPortalQ8RC54.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273068.TTE0593.

Proteomic databases

PRIDEQ8RC54.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM23863; AAM23863; TTE0593.
GeneID996383.
KEGGtte:TTE0593.
PATRIC23895846. VBITheTen82880_0594.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_THETN
AccessionPrimary (citable) accession number: Q8RC54
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: June 1, 2002
Last modified: February 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways