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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781Charge relay systemUniRule annotation
Active sitei153 – 1531Charge relay systemUniRule annotation
Active sitei177 – 1771Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:TTE0607
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000105223Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi273068.TTE0607.

Structurei

3D structure databases

ProteinModelPortaliQ8RC40.
SMRiQ8RC40. Positions 1-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8RC40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELYSLTVHE LKELLHKREV SAQEVTKSYL ERIKEVEPKI DALVTITEEF
60 70 80 90 100
ALERAKVADE MIKNGEAKDL TGIPVIIKDN ISTEGIRTTC SSKMLENYIP
110 120 130 140 150
PYNATVVERL LNEGVVILGK SNLDEFAMGS STENSAFKTT KNPWDLSRVP
160 170 180 190 200
GGSSGGSAAA VAADEAAFAL GSDTGGSIRQ PASLCGVVGM KPTYGLVSRY
210 220 230 240 250
GLVAFASSLD QIGPFTKDVT DCAIVLNAIA GHDPMDSTSV KIEKPDYTSY
260 270 280 290 300
LKEDIKGLRI GVAKEFFGAG IEEGVKETVE KAIKVFEDLG AEIIDISVPY
310 320 330 340 350
VEYALPAYYI IASAEASSNL ARYDGIRYGH IAKNYEDLVD MYMTSRSEGF
360 370 380 390 400
GKEVKRRIML GTYALSSGYY DAYYKKALKV RTLIKNDFER AFEKCDVIVG
410 420 430 440 450
PTSPTVAFKI GERTNDPLAM YLADIYTVSV NIAGLPAISI PCGLSEGLPV
460 470 480
GLQIIGKHFD EGRILNVAYA FEKAYKFDAK PQAIGGER
Length:488
Mass (Da):53,139
Last modified:June 1, 2002 - v1
Checksum:i5902CB61E0622B08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM23877.1.
RefSeqiWP_011025022.1. NC_003869.1.

Genome annotation databases

EnsemblBacteriaiAAM23877; AAM23877; TTE0607.
KEGGitte:TTE0607.
PATRICi23895874. VBITheTen82880_0608.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM23877.1.
RefSeqiWP_011025022.1. NC_003869.1.

3D structure databases

ProteinModelPortaliQ8RC40.
SMRiQ8RC40. Positions 1-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273068.TTE0607.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM23877; AAM23877; TTE0607.
KEGGitte:TTE0607.
PATRICi23895874. VBITheTen82880_0608.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Entry informationi

Entry nameiGATA_CALS4
AccessioniPrimary (citable) accession number: Q8RC40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.