Q8RC39 (GATB_THETN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	TTE0608

Organism

Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]

Taxonomic identifier

273068 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Caldanaerobacter › Caldanaerobacter subterraneus › Caldanaerobacter subterraneus subsp. tengcongensis

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_0000148859

Sequences

	Sequence		Length	Mass (Da)
	Q8RC39 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D24D52CE1C4F49F7	FASTA	475	53,803
	10 20 30 40 50 60 MKYEAVIGLE VHAELLTDSK IFCGCSTKFG SEPNTQVCPV CLGLPGTLPV LNKKVVEYAV 70 80 90 100 110 120 RAGLALNCTI ANFSKMDRKN YFYPDLPKAY QISQYDLPLC SNGYIEIEVE GGTKRIGIKR 130 140 150 160 170 180 IHIEEDAGKL LHEGTDGSLV DYNRAGVPLI EIVSEPDIST PEEAYQYLVK LKSILEYTEV 190 200 210 220 230 240 SDCKMQEGSL RVDTNVSVRP VGTTELGTKI ELKNLNSFKA VQKALEYEIK RQIKVLEEGG 250 260 270 280 290 300 TIVQETRRWN EAKGITEPMR TKEEAHDYRY FPEPDLVPII VTEEWKEEIR KTLPEMPDAK 310 320 330 340 350 360 RERFITQYGL PEYDAKVITS SKKMADFFEK CASNYHSPKI VSNWLMGEFA RLLNDTGKEI 370 380 390 400 410 420 DEVPITPDML IELLKLVDDN VISGSIAKTV FEEMFFTGKN PQIIVEEKGL RQIADEGELR 430 440 450 460 470 RIVRKVIEEN PKSVEDYKKG KEKALGFLVG QVMKETKGKA NPQLTNQLLR EELSK

References

[1]

"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008691 Genomic DNA. Translation: AAM23878.1.
RefSeq	NP_622274.1. NC_003869.1.
3D structure databases
ProteinModelPortal	Q8RC39.
SMR	Q8RC39. Positions 2-400.
ModBase	Search...
Protein-protein interaction databases
STRING	273068.TTE0608.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM23878; AAM23878; TTE0608.
GeneID	996458.
KEGG	tte:TTE0608.
PATRIC	23895876. VBITheTen82880_0609.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223743.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Family and domain databases
Gene3D	1.10.10.410. 1 hit.
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_THETN

Accession

Primary (citable) accession number: Q8RC39

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program