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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurS (PurS), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurL (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei41UniRule annotation1
Binding sitei44ATPUniRule annotation1
Binding sitei83ATPUniRule annotation1
Metal bindingi85Magnesium 1UniRule annotation1
Active sitei87Proton acceptorUniRule annotation1
Binding sitei108SubstrateUniRule annotation1
Metal bindingi109Magnesium 2UniRule annotation1
Binding sitei232SubstrateUniRule annotation1
Metal bindingi260Magnesium 2UniRule annotation1
Binding sitei488ATPUniRule annotation1
Binding sitei525ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi526Magnesium 1UniRule annotation1
Binding sitei528SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Synonyms:purL2
Ordered Locus Names:TTE0811
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001005041 – 733Phosphoribosylformylglycinamidine synthase subunit PurLAdd BLAST733

Proteomic databases

PRIDEiQ8RBK5.

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi273068.TTE0811.

Structurei

3D structure databases

ProteinModelPortaliQ8RBK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 89Substrate bindingUniRule annotation4
Regioni304 – 306Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QIK. Bacteria.
COG0046. LUCA.
HOGENOMiHOG000238227.
KOiK01952.
OMAiVMWQFAE.
OrthoDBiPOG091H009J.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.90.650.10. 1 hit.
HAMAPiMF_00420. PurL_2. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR010074. PRibForGlyAmidine_synth_PurL.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
PIRSFiPIRSF001587. FGAM_synthase_II. 1 hit.
SUPFAMiSSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RBK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIWRELGL TDEEYEKIIS ILGREPNITE LGMYSVMWSE HCSYKNSKPL
60 70 80 90 100
LKYLPTKGER VIQGPGENAG VLDIGDNLAV VMKIESHNHP SAIEPYQGAA
110 120 130 140 150
TGVGGIIRDI FTMGARPIAL LDSLRFGIPD DKRTKYLIEN VVAGIADYGN
160 170 180 190 200
CIGIPTVGGD TYFEESYKGN PLVNAMCVGI VEKDKIKKGI AKGIGNPVMV
210 220 230 240 250
VGATTGRDGI GGASFASQEL SEESEEKRPS VQVGDPFMEK LLLEACLELF
260 270 280 290 300
ETDAVVAIQD MGAAGLTSSS CEMASRGGVG MEIDLDKVPL REEGMTPYEI
310 320 330 340 350
MLSESQERML VVVKKGKEED VQKIFKKWGL NAATIGKITD DGMIRVIKDG
360 370 380 390 400
KIVAEVPAKS LTEDAPQYVR EEEVPKWQEE VKKLDINEVK PPEDMNKVLK
410 420 430 440 450
DVISSLNIAS KEWIYSQYDY MVRTDTVITP GMDAAVVRIK GTRKAIALTT
460 470 480 490 500
DCNGRYCYLD PYIGSQIAVA EAARNLCMVG AKPIGVTDCL NFGNPEKKEI
510 520 530 540 550
YWQLKNSIFG IAKACETFQI PVVSGNVSLY NENEGEAIYP TPVIGMAGLI
560 570 580 590 600
EDVSKICTMD FKKERDVIII LGENKGEIGG SEYLKVCFGM VKGQPPQIDL
610 620 630 640 650
EKEKRLQELV LKLIDEGLIN SSHDISEGGF AAALVESAIA GKKGAKISLQ
660 670 680 690 700
TSLRADIELF SESQSRALIT VSPEKVDEVL KIAYEHQVPA QKVGVVEGKD
710 720 730
IVIDVNGKRI IDLPLEVLEE SWRGRIKWEM ERN
Length:733
Mass (Da):80,476
Last modified:June 1, 2002 - v1
Checksum:i94B747D3AE955F62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM24068.1.
RefSeqiWP_011025205.1. NC_003869.1.

Genome annotation databases

EnsemblBacteriaiAAM24068; AAM24068; TTE0811.
KEGGitte:TTE0811.
PATRICi23896296. VBITheTen82880_0809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM24068.1.
RefSeqiWP_011025205.1. NC_003869.1.

3D structure databases

ProteinModelPortaliQ8RBK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273068.TTE0811.

Proteomic databases

PRIDEiQ8RBK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM24068; AAM24068; TTE0811.
KEGGitte:TTE0811.
PATRICi23896296. VBITheTen82880_0809.

Phylogenomic databases

eggNOGiENOG4107QIK. Bacteria.
COG0046. LUCA.
HOGENOMiHOG000238227.
KOiK01952.
OMAiVMWQFAE.
OrthoDBiPOG091H009J.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.90.650.10. 1 hit.
HAMAPiMF_00420. PurL_2. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR010074. PRibForGlyAmidine_synth_PurL.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
PIRSFiPIRSF001587. FGAM_synthase_II. 1 hit.
SUPFAMiSSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPURL_CALS4
AccessioniPrimary (citable) accession number: Q8RBK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: October 5, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.