Q8RB93 (SYE1_THETN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	TTE0929

Organism

Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]

Taxonomic identifier

273068 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Caldanaerobacter

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000119684
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	244 – 248	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	247	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8RB93 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 37CC69781868C394	FASTA	481	55,544
	10 20 30 40 50 60 MMSEVRVRFA PSPTGSLHIG GARTALFNWL FARHHGGKFI LRVDDTDLER STEESMRGIL 70 80 90 100 110 120 EGLQWLGIDW DEGPIYQSQR LDEYRKFANK LLEEGKAYYC FCTKEELEEM RKQAEREGRP 130 140 150 160 170 180 FMYTGKCRNL TKEQIENYLK EGKKPVIRLK TPREGKTVVH DIIRGDVEFD NSTIDDFIIM 190 200 210 220 230 240 KSDGMPTYNF ATVVDDYQMG ITHIIRAEEH LSNTPKQILI YEALGVPLPQ FAHVSMVLAP 250 260 270 280 290 300 DRTKLSKRHG ATSVQEFRDQ GYLPEAIVNY ITLLGWAPLD GEEIFDVRKS IREFSLERVS 310 320 330 340 350 360 KNPAVYDVQK LTWINGHYIR SYDLDKLTQA IIPFLQKKGL IGENYDYEYI KKIVSVVRER 370 380 390 400 410 420 EKTLVDIADA MTYYFKEVES YEEKGVQKYF TKEGVVDILK KAAETLKNLE PFNKFTAEEA 430 440 450 460 470 480 YRKLVEELGI SSSALFHPTR LAISGRTFGP GLFDIMEFLG KEKTVARIER AIKFIEENIK G

References

[1]	"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed: 11997336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008691 Genomic DNA. Translation: AAM24185.1.
RefSeq	NP_622581.1. NC_003869.1.
3D structure databases
ProteinModelPortal	Q8RB93.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	995885.
GenomeReviews	Gene locus TTE0929 in contig AE008691_GR.
KEGG	tte:TTE0929.
NMPDR	fig|273068.3.peg.227.
PATRIC	23896538. VBITheTen82880_0929.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	NATIIEM.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	TTEN273068:TTE0929-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_THETN

Accession

Primary (citable) accession number: Q8RB93

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program