Reviewed, UniProtKB/Swiss-Prot Q8RB77 (GPR_THETN)

Names and origin

Protein names

Recommended name:
    Germination protease
    EC=3.4.24.78
Alternative name(s):
    Spore protease
    GPR endopeptidase
    Germination proteinase

Gene names

Name:	gpr
Ordered Locus Names:	TTE0946

Organism

Thermoanaerobacter tengcongensis [Complete proteome] [HAMAP]

Taxonomic identifier

119072 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Thermoanaerobacter

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Initiates the rapid degradation of small, acid-soluble proteins during spore germination By similarity.
Catalytic activity	Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala. HAMAP MF_00626
Subunit structure	Homotetramer By similarity.
Post-translational modification	Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination By similarity.
Sequence similarities	Belongs to the peptidase A25 family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP spore germination Inferred from electronic annotation. Source: HAMAP
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 10	10	By similarity		PRO_0000026880
	Chain	11 – 324	314	Germination protease HAMAP MF_00626		PRO_0000026881

Sequences

	Sequence		Length	Mass (Da)
	Q8RB77-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D4E8B6C5763C3D7C	FASTA	324	35,519
	10 20 30 40 50 60 MIIVLGIRTD LALEARELYK EREIPGVSIR EEGEEGIKIT RVKILDERGE KAMGKPVGDY 70 80 90 100 110 120 ITIEAPGLLE RDLDLEERVA KVLANIISEL AQLKKDSHVL VVGLGNWNVT PDALGPRVVS 130 140 150 160 170 180 NIVVTRHLKE YAPLQFGDEI RSVSAFSPGV LGITGIETAE ILKGVVDRVK PDLVITIDAL 190 200 210 220 230 240 ASRRLERLST TIQISNTGIS PGSGVGNRRL SITSESLGVP VIAIGVPTVV DAVTIAHDTI 250 260 270 280 290 300 EYLVKELSEQ TSKESVFYKV LENMNKQEKY SLIEEVLSPY VQNLVVTPKE IDLLIKNIAL 310 320 VISRGINLAL QPGLTEKEMN QLLH

References

[1]

"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed: 11997336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013060 Genomic DNA. Translation: AAM24202.1.
RefSeq	NP_622598.1.
3D structure databases
HSSP	HSSP built from PDB template 1C8B based on UniProtKB P22321.
ModBase	Search...
Genome annotation databases
GeneID	995860.
GenomeReviews	Gene locus TTE0946 in contig AE008691_GR.
KEGG	tte:TTE0946.
NMPDR	fig|273068.3.peg.337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8RB77.
OMA	Q8RB77. LMVTRHL.
Enzyme and pathway databases
BioCyc	TTEN273068:TTE0946-MON.
BRENDA	3.4.24.78. 276887.
Family and domain databases
HAMAP	MF_00626. [Tree]
InterPro	IPR005080. Peptidase_A25. [Graphical view]
Gene3D	G3DSA:3.40.50.10100. Peptidase_A25. 1 hit.
Pfam	PF03418. Peptidase_A25. 1 hit. [Graphical view]
PIRSF	PIRSF019549. Peptidase_A25. 1 hit.
ProDom	PD041835. Peptidase_A25. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01441. GPR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPR_THETN

Accession

Primary (citable) accession number: Q8RB77

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)