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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941NucleophileUniRule annotation
Binding sitei95 – 951SubstrateUniRule annotation
Metal bindingi306 – 3061ZincUniRule annotation
Metal bindingi308 – 3081ZincUniRule annotation
Metal bindingi311 – 3111ZincUniRule annotation
Metal bindingi337 – 3371ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:TTE1183
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_0000135548Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273068.TTE1183.

Structurei

3D structure databases

ProteinModelPortaliQ8RAM9.
SMRiQ8RAM9. Positions 4-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiGIDLFDC.
OrthoDBiPOG091H00XO.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8RAM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIKYQVIK KDARTKARLG ILETPHGVIE TPVFMPVGTQ ATVKAMTPDE
60 70 80 90 100
LKEMGATIIL GNTYHLYLRP GHKIIEKAGG LHKFMNWDRA ILTDSGGFQV
110 120 130 140 150
FSLSSLRKIT EDGVEFRSHI DGSKHFFTPE KVIEIQNSLG SDIIMSFDEC
160 170 180 190 200
APYPADYDYV KRSMELTIKW AKRGKKAHKN TDRQALFGIV QGGTYKDLRR
210 220 230 240 250
ECAERLVDMD FPGYAIGGLS VGEPKDLMYE IIDFTTDYLP HDKPRYLMGV
260 270 280 290 300
GTPEDLIEGV IRGVDMFDCV LPTRIARNGT VFTSRGKLIV RDAPYAEDFS
310 320 330 340 350
PLDEECDCYT CKNYSRAYLR HLFKAKEILA ARLATYHNLY FLIKLMEKIR
360 370
EAIRQDRLLE FKEEFLKKYY GNREE
Length:375
Mass (Da):42,931
Last modified:June 1, 2002 - v1
Checksum:iF11371CAFBE1B00F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM24414.1.
RefSeqiWP_011025518.1. NC_003869.1.

Genome annotation databases

EnsemblBacteriaiAAM24414; AAM24414; TTE1183.
KEGGitte:TTE1183.
PATRICi23897069. VBITheTen82880_1189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM24414.1.
RefSeqiWP_011025518.1. NC_003869.1.

3D structure databases

ProteinModelPortaliQ8RAM9.
SMRiQ8RAM9. Positions 4-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273068.TTE1183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM24414; AAM24414; TTE1183.
KEGGitte:TTE1183.
PATRICi23897069. VBITheTen82880_1189.

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiGIDLFDC.
OrthoDBiPOG091H00XO.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTGT_CALS4
AccessioniPrimary (citable) accession number: Q8RAM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.