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Q8R9Y1 (Q8R9Y1_THETN) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length694 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q8R9Y1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 451D03665BC10E3B

FASTA69479,055
        10         20         30         40         50         60 
MAKSLVIVES PAKAKTISKF LGKNFKVAAS QGHVRDLPKS QLGVDIENNF APKYITIRGK 

        70         80         90        100        110        120 
GEIIEKLKKE AKDAEKIFLA TDPDREGEAI SWHIAQLLDL DIDKPCRIEF HEITKNAVQA 

       130        140        150        160        170        180 
AIKNPRKIDL NLVNAQQARR ILDRLVGYKI SPILWKKIKR GLSAGRVQSV AARLVCDRER 

       190        200        210        220        230        240 
EIEAFVPEEY WNITAVLFDE KTSSKFEAKF YGREEEKIEL KNREEVDKII NSLSEEYVVY 

       250        260        270        280        290        300 
KVKTGTKKRN PPPPFITSTL QQEASRKLGF TAKKTMLIAQ QLYEGIEIKG EGSVGLVTYI 

       310        320        330        340        350        360 
RTDSTRVAKE AKEEAYRYIT EKFGKEYANP DAEYSSKKAN VQDAHEAIRP TSVFREPEII 

       370        380        390        400        410        420 
KDSLTPDQYK LYKLIWDRFI ASQMMPAIYD TVAMDIKNNG YIFKASGSSV KFLGFMTVYV 

       430        440        450        460        470        480 
EGSDFQEEEE EKTLPVLKEG TKVRLEELKP TQHFTQPPPR YTEASLIKEL EEKGIGRPST 

       490        500        510        520        530        540 
YAPTIATLLE RGYVVKDKKV LKPTELGFIV TDALKEFFSE IVDVGFTAEM EEQLDKIEEG 

       550        560        570        580        590        600 
KVEWYEVVGD FYRDFYKTLK VAEEQMEKVE LEEEVEVTDI KCEVCGRPMV VRKGRYGKFL 

       610        620        630        640        650        660 
ACSGFPECKN TKPLLEKVGV KCPKCGGEIV KRRSKKGRVY YSCENAPQCD FVLWDKPVNE 

       670        680        690 
KCPVCGSLMV EKNTKNGIVI KCSNPECGYQ KETN

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References

[1]"A complete sequence of the T. tengcongensis genome."
Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. expand/collapse author list , Wang J., Yu J., Yang H.
Genome Res. 12:689-700(2002) [PubMed: 11997336] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE008691 Genomic DNA. Translation: AAM24671.1.
RefSeqNP_623067.1. NC_003869.1.

3D structure databases

HSSPHSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortalQ8R9Y1.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID997890.
GenomeReviewsGene locus TTE1449 in contig AE008691_GR.
KEGGtte:TTE1449.
NMPDRfig|273068.3.peg.1414.
PATRIC23897610. VBITheTen82880_1458.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG512935.
OMATIPKERE.
ProtClustDBPRK05582.

Enzyme and pathway databases

BioCycTTEN273068:TTE1449-MONOMER.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ8R9Y1_THETN
AccessionPrimary (citable) accession number: Q8R9Y1
Entry history
Integrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info