Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132L-tryptophanUniRule annotation1
Binding sitei183ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 11ATPUniRule annotation3
Nucleotide bindingi17 – 18ATPUniRule annotation2
Nucleotide bindingi144 – 146ATPUniRule annotation3
Nucleotide bindingi192 – 196ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:TTE1453
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367001 – 327Tryptophan--tRNA ligaseAdd BLAST327

Proteomic databases

PRIDEiQ8R9X8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273068.TTE1453

Structurei

3D structure databases

ProteinModelPortaliQ8R9X8
SMRiQ8R9X8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 18"HIGH" regionUniRule annotation9
Motifi192 – 196"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R9X8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVFSGVQP SGDIHIGNYL GAMRQFVALQ DDYDCFFCVV DLHALTVPQD
60 70 80 90 100
PVELKKNTIE LAALYMAIGL DPKKVTLFVQ SHVSAHAELA WLLQCITYFG
110 120 130 140 150
ELSRMTQFKE KSKGKESVSV GLFTYPDLMA ADILLYKTHY VPVGEDQKQH
160 170 180 190 200
LELTRDVAQR FNNRFGETFV IPEPMILKFG ARIMSLTNPT KKMSKSDADP
210 220 230 240 250
NNRVNLLDDP DTIYRKIMKA VTDSESEIRL DWEKKPGISN LLTIYSLFTG
260 270 280 290 300
MEVDEVVNKF KGQGYGTLKK ELAEVVIDKL SVIQKNYRDI SEEEVLRVLK
310 320
EGAERAEAVA VETLKEVKEK MGLILRD
Length:327
Mass (Da):37,092
Last modified:June 1, 2002 - v1
Checksum:i44ED1BA7AAA71165
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA Translation: AAM24675.1
RefSeqiWP_011025722.1, NC_003869.1

Genome annotation databases

EnsemblBacteriaiAAM24675; AAM24675; TTE1453
KEGGitte:TTE1453

Similar proteinsi

Entry informationi

Entry nameiSYW_CALS4
AccessioniPrimary (citable) accession number: Q8R9X8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health