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Q8R9S4 (DAPF_THETN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Diaminopimelate epimerase HAMAP-Rule MF_00197
PRO_0000149874

Regions

Region8 – 92Substrate binding By similarity
Region69 – 713Substrate binding By similarity
Region209 – 2102Substrate binding By similarity

Sites

Active site691Proton donor/acceptor By similarity
Active site2181Proton donor/acceptor By similarity
Binding site111Substrate By similarity
Binding site421Substrate By similarity
Binding site601Substrate By similarity
Binding site1911Substrate By similarity
Site1601Important for catalytic activity By similarity
Site2091Important for catalytic activity By similarity

Amino acid modifications

Disulfide bond69 ↔ 218 HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
Q8R9S4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 941D7FCB35774CCC

FASTA27430,259
        10         20         30         40         50         60 
MRFTKMHGLG NDFIVIEAVE GVDYSELAVK LCDRHFGIGA DGLLVVEPSH IADIKMRIFN 

        70         80         90        100        110        120 
ADGSEAEMCG NGSRCFAKYV YEKGIVSKQK MTVETLAGVI MPELFVENGK IKSVKVYMGS 

       130        140        150        160        170        180 
PIFESSKIPV KSEKQKFIDE PVKIDGKTYR LSSVRVGVPH TILFVSSFEE SFMKELGPKI 

       190        200        210        220        230        240 
EKSSLFPEGT NVDFVKVEDE ENISVRTWER GVGLTLACGS GASASAVVSS LLGRTRRSVN 

       250        260        270 
VHFKAGVLLV EWKEDNSIYL SGEVEEVFRG EIEI 

« Hide

References

[1]"A complete sequence of the T. tengcongensis genome."
Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. expand/collapse author list , Wang J., Yu J., Yang H.
Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008691 Genomic DNA. Translation: AAM24731.1.
RefSeqNP_623127.1. NC_003869.1.

3D structure databases

ProteinModelPortalQ8R9S4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273068.TTE1514.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM24731; AAM24731; TTE1514.
GeneID997930.
KEGGtte:TTE1514.
PATRIC23897738. VBITheTen82880_1522.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0253.
HOGENOMHOG000220466.
KOK01778.
OMAKMRIFNN.
OrthoDBEOG6ND0M5.

Enzyme and pathway databases

UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689. PTHR31689. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPF_THETN
AccessionPrimary (citable) accession number: Q8R9S4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways