Q8R9P3 (QUEF_THETN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	TTE1563

Organism

Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]

Taxonomic identifier

273068 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Caldanaerobacter

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000163014

Sequences

	Sequence		Length	Mass (Da)
	Q8R9P3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2EF733D425601B61	FASTA	134	16,088
	10 20 30 40 50 60 MTDKYKERRF DIYGYEKIDK EVLESIEYEY PEKNTIVEYI TDEFSSVCPW TGLPDNAKLT 70 80 90 100 110 120 IRYIPHKKLV ELKSLKYYLT SYRNVGILQE HAINRILDDL VEFLQPKFME IIGEFQERGG 130 IATRIIARYE KEEY

References

[1]

"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed: 11997336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008691 Genomic DNA. Translation: AAM24767.1.
RefSeq	NP_623163.1. NC_003869.1.
3D structure databases
ProteinModelPortal	Q8R9P3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	995665.
GenomeReviews	Gene locus TTE1563 in contig AE008691_GR.
KEGG	tte:TTE1563.
NMPDR	fig|273068.3.peg.1510.
PATRIC	23897826. VBITheTen82880_1562.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG294103.
OMA	HEESANQ.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	TTEN273068:TTE1563-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_THETN

Accession

Primary (citable) accession number: Q8R9P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program