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Q8R9L3

- SYI_THETN

UniProt

Q8R9L3 - SYI_THETN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi899 – 8991ZincUniRule annotation
    Metal bindingi902 – 9021ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation
    Metal bindingi922 – 9221ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TTE1594
    OrganismiThermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis)
    Taxonomic identifieri273068 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
    ProteomesiUP000000555: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 932932Isoleucine--tRNA ligasePRO_0000098493Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273068.TTE1594.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R9L3.
    SMRiQ8R9L3. Positions 1-929.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R9L3-1 [UniParc]FASTAAdd to Basket

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    MDYNKTLNLP KTDFPMKANL PVREPEILKK WEEMDIYRRV LEKNKGKEKY    50
    ILHDGPPYAN GDIHIGTAMN KVLKDIVVKY KTMRGYDSPY VPGWDTHGLP 100
    IEQQVIKILG VKRHEMNPVE FRKVCKDFAF SQIERQRQQF KRLGVRGDWE 150
    KPYLTLDPEY EAKQIEVFGE MAKRGYIYKG LKPVYWCPSC ETALAEAEIE 200
    YFDETSDSIY VKFRVRDDLG KFKGIVEDLS NVYFVIWTTT TWTIPANLAI 250
    ALNPEFDYSL TKFGDEVYII AKDMIEEVRK ETGLGDYEIL ATFKGKELEG 300
    MKAVHPIYNR DSIVVLGEHV TLDAGTGCVH TAPGHGEEDF LVGQEYGLEA 350
    LNPIDEKGYF TDKAPGYEGL YYAEANKVIK EDLRKANALL AEKKITHSYP 400
    HCWRCKSPIL FRATEQWFAS VEGFREEALK AIKEVNWYPA WGEERITNMV 450
    RDRKDWCISR QRVWGVPIPI FYCENCGKPL INDETINAVK ELFRQKGSDA 500
    WFEMSAEEIL PEGIKCECGS TKFRKETDIM DVWFDSGSSH AAVLETHPDL 550
    KWPAELYLEG SDQHRGWFQS SLLTAVATRG KAPYKNVLTH GFVVDGEGRK 600
    MSKSLGNVVD PAEVIQEYGA DVLRLWVVSA DYTADMRTSP EILKQIAEVY 650
    RKIRNTARFL LGNLYDFDPD KDMLPYEELL EIDKWALYRL NRLVKQLTES 700
    FDKYEFYDFF HPVHNFCVVD MSSLYLDILK DRLYTYPAKS KERKAAQTTL 750
    YIILDTLVKL MAPVLAFTSE EIWWHMKHDR NNNFESVQLA DWPQVKEEYD 800
    NPYIIEKWEK LFDIRKDISK ALEIARSEKK IGHSLDAQVD IYPSSELYEF 850
    FKGFEDLQYV FIVSKVVLHD PQEVAPENAY VSEDYDLKIT VTRAPGEKCE 900
    RCWMYSETVG TIKEHPTICA RCASHIEEQL KV 932
    Length:932
    Mass (Da):108,136
    Last modified:June 1, 2002 - v1
    Checksum:i9CB12C99B76D09C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008691 Genomic DNA. Translation: AAM24798.1.
    RefSeqiNP_623194.1. NC_003869.1.

    Genome annotation databases

    EnsemblBacteriaiAAM24798; AAM24798; TTE1594.
    GeneIDi996077.
    KEGGitte:TTE1594.
    PATRICi23897890. VBITheTen82880_1594.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008691 Genomic DNA. Translation: AAM24798.1 .
    RefSeqi NP_623194.1. NC_003869.1.

    3D structure databases

    ProteinModelPortali Q8R9L3.
    SMRi Q8R9L3. Positions 1-929.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273068.TTE1594.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM24798 ; AAM24798 ; TTE1594 .
    GeneIDi 996077.
    KEGGi tte:TTE1594.
    PATRICi 23897890. VBITheTen82880_1594.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

    Entry informationi

    Entry nameiSYI_THETN
    AccessioniPrimary (citable) accession number: Q8R9L3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3