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Q8R9L3

- SYI_CALS4

UniProt

Q8R9L3 - SYI_CALS4

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TTE1594
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
ProteomesiUP000000555: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligasePRO_0000098493Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi273068.TTE1594.

Structurei

3D structure databases

ProteinModelPortaliQ8R9L3.
SMRiQ8R9L3. Positions 1-929.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R9L3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYNKTLNLP KTDFPMKANL PVREPEILKK WEEMDIYRRV LEKNKGKEKY
60 70 80 90 100
ILHDGPPYAN GDIHIGTAMN KVLKDIVVKY KTMRGYDSPY VPGWDTHGLP
110 120 130 140 150
IEQQVIKILG VKRHEMNPVE FRKVCKDFAF SQIERQRQQF KRLGVRGDWE
160 170 180 190 200
KPYLTLDPEY EAKQIEVFGE MAKRGYIYKG LKPVYWCPSC ETALAEAEIE
210 220 230 240 250
YFDETSDSIY VKFRVRDDLG KFKGIVEDLS NVYFVIWTTT TWTIPANLAI
260 270 280 290 300
ALNPEFDYSL TKFGDEVYII AKDMIEEVRK ETGLGDYEIL ATFKGKELEG
310 320 330 340 350
MKAVHPIYNR DSIVVLGEHV TLDAGTGCVH TAPGHGEEDF LVGQEYGLEA
360 370 380 390 400
LNPIDEKGYF TDKAPGYEGL YYAEANKVIK EDLRKANALL AEKKITHSYP
410 420 430 440 450
HCWRCKSPIL FRATEQWFAS VEGFREEALK AIKEVNWYPA WGEERITNMV
460 470 480 490 500
RDRKDWCISR QRVWGVPIPI FYCENCGKPL INDETINAVK ELFRQKGSDA
510 520 530 540 550
WFEMSAEEIL PEGIKCECGS TKFRKETDIM DVWFDSGSSH AAVLETHPDL
560 570 580 590 600
KWPAELYLEG SDQHRGWFQS SLLTAVATRG KAPYKNVLTH GFVVDGEGRK
610 620 630 640 650
MSKSLGNVVD PAEVIQEYGA DVLRLWVVSA DYTADMRTSP EILKQIAEVY
660 670 680 690 700
RKIRNTARFL LGNLYDFDPD KDMLPYEELL EIDKWALYRL NRLVKQLTES
710 720 730 740 750
FDKYEFYDFF HPVHNFCVVD MSSLYLDILK DRLYTYPAKS KERKAAQTTL
760 770 780 790 800
YIILDTLVKL MAPVLAFTSE EIWWHMKHDR NNNFESVQLA DWPQVKEEYD
810 820 830 840 850
NPYIIEKWEK LFDIRKDISK ALEIARSEKK IGHSLDAQVD IYPSSELYEF
860 870 880 890 900
FKGFEDLQYV FIVSKVVLHD PQEVAPENAY VSEDYDLKIT VTRAPGEKCE
910 920 930
RCWMYSETVG TIKEHPTICA RCASHIEEQL KV
Length:932
Mass (Da):108,136
Last modified:June 1, 2002 - v1
Checksum:i9CB12C99B76D09C7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008691 Genomic DNA. Translation: AAM24798.1.
RefSeqiNP_623194.1. NC_003869.1.

Genome annotation databases

EnsemblBacteriaiAAM24798; AAM24798; TTE1594.
GeneIDi996077.
KEGGitte:TTE1594.
PATRICi23897890. VBITheTen82880_1594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008691 Genomic DNA. Translation: AAM24798.1 .
RefSeqi NP_623194.1. NC_003869.1.

3D structure databases

ProteinModelPortali Q8R9L3.
SMRi Q8R9L3. Positions 1-929.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273068.TTE1594.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM24798 ; AAM24798 ; TTE1594 .
GeneIDi 996077.
KEGGi tte:TTE1594.
PATRICi 23897890. VBITheTen82880_1594.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Entry informationi

Entry nameiSYI_CALS4
AccessioniPrimary (citable) accession number: Q8R9L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3