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Q8R9L3

- SYI_THETN

UniProt

Q8R9L3 - SYI_THETN

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Protein

Isoleucine--tRNA ligase

Gene
ileS, TTE1594
Organism
Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei603 – 6031ATP By similarity
Metal bindingi899 – 8991Zinc By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi922 – 9221Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:TTE1594
OrganismiThermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
ProteomesiUP000000555: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligaseUniRule annotationPRO_0000098493Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi273068.TTE1594.

Structurei

3D structure databases

ProteinModelPortaliQ8R9L3.
SMRiQ8R9L3. Positions 1-929.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R9L3-1 [UniParc]FASTAAdd to Basket

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MDYNKTLNLP KTDFPMKANL PVREPEILKK WEEMDIYRRV LEKNKGKEKY    50
ILHDGPPYAN GDIHIGTAMN KVLKDIVVKY KTMRGYDSPY VPGWDTHGLP 100
IEQQVIKILG VKRHEMNPVE FRKVCKDFAF SQIERQRQQF KRLGVRGDWE 150
KPYLTLDPEY EAKQIEVFGE MAKRGYIYKG LKPVYWCPSC ETALAEAEIE 200
YFDETSDSIY VKFRVRDDLG KFKGIVEDLS NVYFVIWTTT TWTIPANLAI 250
ALNPEFDYSL TKFGDEVYII AKDMIEEVRK ETGLGDYEIL ATFKGKELEG 300
MKAVHPIYNR DSIVVLGEHV TLDAGTGCVH TAPGHGEEDF LVGQEYGLEA 350
LNPIDEKGYF TDKAPGYEGL YYAEANKVIK EDLRKANALL AEKKITHSYP 400
HCWRCKSPIL FRATEQWFAS VEGFREEALK AIKEVNWYPA WGEERITNMV 450
RDRKDWCISR QRVWGVPIPI FYCENCGKPL INDETINAVK ELFRQKGSDA 500
WFEMSAEEIL PEGIKCECGS TKFRKETDIM DVWFDSGSSH AAVLETHPDL 550
KWPAELYLEG SDQHRGWFQS SLLTAVATRG KAPYKNVLTH GFVVDGEGRK 600
MSKSLGNVVD PAEVIQEYGA DVLRLWVVSA DYTADMRTSP EILKQIAEVY 650
RKIRNTARFL LGNLYDFDPD KDMLPYEELL EIDKWALYRL NRLVKQLTES 700
FDKYEFYDFF HPVHNFCVVD MSSLYLDILK DRLYTYPAKS KERKAAQTTL 750
YIILDTLVKL MAPVLAFTSE EIWWHMKHDR NNNFESVQLA DWPQVKEEYD 800
NPYIIEKWEK LFDIRKDISK ALEIARSEKK IGHSLDAQVD IYPSSELYEF 850
FKGFEDLQYV FIVSKVVLHD PQEVAPENAY VSEDYDLKIT VTRAPGEKCE 900
RCWMYSETVG TIKEHPTICA RCASHIEEQL KV 932
Length:932
Mass (Da):108,136
Last modified:June 1, 2002 - v1
Checksum:i9CB12C99B76D09C7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008691 Genomic DNA. Translation: AAM24798.1.
RefSeqiNP_623194.1. NC_003869.1.

Genome annotation databases

EnsemblBacteriaiAAM24798; AAM24798; TTE1594.
GeneIDi996077.
KEGGitte:TTE1594.
PATRICi23897890. VBITheTen82880_1594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008691 Genomic DNA. Translation: AAM24798.1 .
RefSeqi NP_623194.1. NC_003869.1.

3D structure databases

ProteinModelPortali Q8R9L3.
SMRi Q8R9L3. Positions 1-929.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273068.TTE1594.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM24798 ; AAM24798 ; TTE1594 .
GeneIDi 996077.
KEGGi tte:TTE1594.
PATRICi 23897890. VBITheTen82880_1594.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Entry informationi

Entry nameiSYI_THETN
AccessioniPrimary (citable) accession number: Q8R9L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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