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Q8R9G2 (MURE_THETN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene names
Name:murE
Synonyms:murE2
Ordered Locus Names:TTE1649
OrganismThermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]
Taxonomic identifier273068 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Caldanaerobacter ›

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP-Rule MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP-Rule MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00208

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP-Rule MF_00208

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Sequence caution

The sequence AAM24851.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP-Rule MF_00208
PRO_0000101963

Regions

Nucleotide binding109 – 1157ATP Potential
Region151 – 1522UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region399 – 4024Meso-diaminopimelate binding By similarity
Motif399 – 4024Meso-diaminopimelate recognition motif HAMAP-Rule MF_00208

Sites

Binding site291UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1781UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1861UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site3751Meso-diaminopimelate By similarity
Binding site4511Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site4551Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2181N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R9G2 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: FC335F3D615FB460

FASTA48253,649
        10         20         30         40         50         60 
MRLVDLLKGV KHEIKGNPNV DISGVCYDSR KAKPKYLFIA IKGFKTDGLL YVEEAIKNGA 

        70         80         90        100        110        120 
VAVVTDRDIS EYPGVTVVLV EDARAAMAKI ASNFYNNPTS KLTLIGITGT NGKTSVTYML 

       130        140        150        160        170        180 
KAILEQQNNK VGLVGTIQNM IGDRVIPTTH TTPESLDLQE LFSLMVNEGV KYVVMEVSSH 

       190        200        210        220        230        240 
SLALHRVDSC DFDIAVFTNL SQDHLDFHES MEEYAKTKSK LFKMAKKASV INIDDKYSSM 

       250        260        270        280        290        300 
MIESSKGKVL TYGIKDFAYV MAKEIKNSLS GVKFKVQIQD KKEEISLKIP GLFSVYNALA 

       310        320        330        340        350        360 
AITVADFLGI PLRSVREALS HVTVKGRFEP VETGRDFYVF IDYAHTPDGI RNIMEALKEY 

       370        380        390        400        410        420 
EAGRKILVFG AGGDRDKSKR PLMGEVAGKY ADFCILTSDN PRSENPKEII AQIEEGIKKT 

       430        440        450        460        470        480 
NCPYVVIEDR REAIRYALSN AQKDDVIILA GKGHETYQII GDKVIPFDER EIVKEILAES 


EK

« Hide

References

[1]"A complete sequence of the T. tengcongensis genome."
Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. expand/collapse author list , Wang J., Yu J., Yang H.
Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE008691 Genomic DNA. Translation: AAM24851.1. Different initiation.
RefSeqNP_623247.1. NC_003869.1.

3D structure databases

ProteinModelPortalQ8R9G2.
ModBaseSearch...

Protein-protein interaction databases

STRING273068.TTE1649.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM24851; AAM24851; TTE1649.
GeneID996203.
KEGGtte:TTE1649.
PATRIC23898004. VBITheTen82880_1650.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0769.
HOGENOMHOG000268118.
KOK01928.
OMARPLMGEA.
ProtClustDBPRK00139.

Enzyme and pathway databases

BioCycCSUB273068:GJEB-2979-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPMF_00208. MurE.
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01085. murE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURE_THETN
AccessionPrimary (citable) accession number: Q8R9G2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: April 30, 2003
Last modified: May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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