Q8R967 (ENO_THETN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enolase
EC=4.2.1.11

Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase

Gene names

Name:	eno
Ordered Locus Names:	TTE1759

Organism

Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]

Taxonomic identifier

273068 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Caldanaerobacter › Caldanaerobacter subterraneus › Caldanaerobacter subterraneus subsp. tengcongensis

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis By similarity. HAMAP-Rule MF_00318
Catalytic activity	2-phospho-D-glycerate = phosphoenolpyruvate + H2O. HAMAP-Rule MF_00318
Cofactor	Magnesium. Required for catalysis and for stabilizing the dimer By similarity.
Enzyme regulation	The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein By similarity. HAMAP-Rule MF_00318
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. HAMAP-Rule MF_00318
Subcellular location	Cytoplasm. Secreted. Cell surface. Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial cell surface By similarity. HAMAP-Rule MF_00318
Sequence similarities	Belongs to the enolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm Secreted
Ligand	Magnesium Metal-binding
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphopyruvate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Enolase HAMAP-Rule MF_00318		PRO_0000133997
Regions
	Region	365 – 368	4	Substrate binding By similarity
Sites
	Active site	205	1	Proton donor By similarity
	Active site	338	1	Proton acceptor By similarity
	Metal binding	242	1	Magnesium By similarity
	Metal binding	286	1	Magnesium By similarity
	Metal binding	313	1	Magnesium By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	286	1	Substrate By similarity
	Binding site	313	1	Substrate By similarity
	Binding site	338	1	Substrate (covalent); in inhibited form By similarity
	Binding site	389	1	Substrate By similarity
Amino acid modifications
	Modified residue	280	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8R967 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: ABCC2445DEBA8D65	FASTA	429	46,297
	10 20 30 40 50 60 MSSIIDIYAR EILDSRGNPT IEVEVELDSG AVGRAAVPSG ASTGAFEAIE LRDGDKSRYL 70 80 90 100 110 120 GKGVLKAVQN VNDIIAPELI GMEAQDQVAI DKAMIELDGT PNKSKLGANA ILGVSLAVAK 130 140 150 160 170 180 AAAEELGLPL YQYLGGVNAK TLPVPMMNIL NGGKHADNNV DIQEFMIMPV GAPNFKEALR 190 200 210 220 230 240 MCSEVYHSLK NVLHSKGLST TVGDEGGFAP NLTSNEEAIK VILEAIEKAG YVPGEDIVLA 250 260 270 280 290 300 LDPAATEMYK EDGKYHFEGE GIVRTSEEMI EFWEQLVSKY PIVSIEDGLA EEDWNGWKLL 310 320 330 340 350 360 TERLGKKVQL VGDDLFVTNT ERLSKGINMG VANSILIKLN QIGTLTETLD AIEMAKRAGY 370 380 390 400 410 420 TAIVSHRSGE TEDTTIADLV VATNVGQIKT GAPARTDRVA KYNQLLRIEE ALGSVAQYPG KNAFYNIRK

References

[1]

"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008691 Genomic DNA. Translation: AAM24953.1.
RefSeq	NP_623349.1. NC_003869.1.
3D structure databases
ProteinModelPortal	Q8R967.
SMR	Q8R967. Positions 2-428.
ModBase	Search...
Protein-protein interaction databases
STRING	273068.TTE1759.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM24953; AAM24953; TTE1759.
GeneID	996800.
KEGG	tte:TTE1759.
PATRIC	23898261. VBITheTen82880_1768.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0148.
HOGENOM	HOG000072173.
KO	K01689.
OMA	EYMIMPL.
ProtClustDB	PRK00077.
Enzyme and pathway databases
UniPathway	UPA00109; UER00187.
Family and domain databases
HAMAP	MF_00318. Enolase.
InterPro	IPR000941. Enolase. IPR020810. Enolase_C. IPR020809. Enolase_CS. IPR020811. Enolase_N. [Graphical view]
PANTHER	PTHR11902. PTHR11902. 1 hit.
Pfam	PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF001400. Enolase. 1 hit.
PRINTS	PR00148. ENOLASE.
TIGRFAMs	TIGR01060. eno. 1 hit.
PROSITE	PS00164. ENOLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENO_THETN

Accession

Primary (citable) accession number: Q8R967

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program