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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521SubstrateUniRule annotation
Binding sitei186 – 1861SubstrateUniRule annotation
Metal bindingi192 – 1921ManganeseUniRule annotation
Binding sitei192 – 1921ATPUniRule annotation
Binding sitei192 – 1921SubstrateUniRule annotation
Metal bindingi211 – 2111Manganese; via tele nitrogenUniRule annotation
Binding sitei211 – 2111ATPUniRule annotation
Metal bindingi248 – 2481ManganeseUniRule annotation
Binding sitei276 – 2761ATPUniRule annotation
Binding sitei313 – 3131ATPUniRule annotation
Binding sitei313 – 3131SubstrateUniRule annotation
Binding sitei438 – 4381ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi227 – 2359ATPUniRule annotation
Nucleotide bindingi432 – 4332ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:TTE1783
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Phosphoenolpyruvate carboxykinase [ATP]PRO_0000203853Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273068.TTE1783.

Structurei

3D structure databases

ProteinModelPortaliQ8R943.
SMRiQ8R943. Positions 3-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNLERLGLI NFKNVYRNLP VAKLIEEAVK REEGVLVNNG AFNVYTGKYT
60 70 80 90 100
GRSPNDKFIV DEPEVHEDIW WENNKPISVE KFEKLYNRLI AYLQNRDLFI
110 120 130 140 150
FDGFVGADPQ YRVSIRVITE YAYQSLMARQ LFIRPTEKEL QNFVPEYTLI
160 170 180 190 200
AAPRFKAIPE IDGVNSEAFI ILSFTKKLII IGGTQYGGEI KKSIFTLMNY
210 220 230 240 250
LMPKKGVLSM HCSANIGKDG DTALFFGLSG TGKTTLSADP ERFLIGDDEH
260 270 280 290 300
GWSERGIFNF EGGCYAKCIN LSREKEPQIW DSIRFGAILE NVVYDEATRE
310 320 330 340 350
LDYTSDKITE NTRAAYPIDF IPGAVQSGIG GHPKTIIFLT ADAFGVLPPI
360 370 380 390 400
AKLTKEQAMY YFLSGYTSKL AGTERGITEP QATFSTCFGA PFLPLPPMVY
410 420 430 440 450
AKMLGEKIEK YDTKVFLVNT GWSGGPYGIG KRINLEYTRK MVSAALKGEL
460 470 480 490 500
DKVEFTKDPI FNLNIPASCP GVPSEILNPR NTWKDKEEYD KTAKRLAQRF
510 520
IENFQKYKEV SEEIKNAGPK G
Length:521
Mass (Da):58,771
Last modified:June 1, 2002 - v1
Checksum:i1783A3320B106341
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM24977.1.
RefSeqiWP_011025979.1. NC_003869.1.

Genome annotation databases

EnsemblBacteriaiAAM24977; AAM24977; TTE1783.
KEGGitte:TTE1783.
PATRICi23898309. VBITheTen82880_1792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM24977.1.
RefSeqiWP_011025979.1. NC_003869.1.

3D structure databases

ProteinModelPortaliQ8R943.
SMRiQ8R943. Positions 3-520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273068.TTE1783.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM24977; AAM24977; TTE1783.
KEGGitte:TTE1783.
PATRICi23898309. VBITheTen82880_1792.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OrthoDBiEOG6DG2RK.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Entry informationi

Entry nameiPCKA_CALS4
AccessioniPrimary (citable) accession number: Q8R943
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.