Reviewed, UniProtKB/Swiss-Prot Q8R886 (HIS2_THETN)

Names and origin

Protein names

Recommended name:
    Histidine biosynthesis bifunctional protein hisIE
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosyl-AMP cyclohydrolase
                Short name=PRA-CH
              EC=3.5.4.19
    2- Recommended name:
            Phosphoribosyl-ATP pyrophosphatase
                Short name=PRA-PH
              EC=3.6.1.31

Gene names

Name:	hisI
Synonyms:	hisIE
Ordered Locus Names:	TTE2132

Organism

Thermoanaerobacter tengcongensis [Complete proteome] [HAMAP]

Taxonomic identifier

119072 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Thermoanaerobacter

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-ATP + H2O = 1-(5-phosphoribosyl)-AMP + diphosphate. HAMAP MF_01019 1-(5-phosphoribosyl)-AMP + H2O = 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide. HAMAP MF_01019
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9. HAMAP MF_01019 Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the PRA-CH family. In the C-terminal section; belongs to the PRA-PH family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosyl-AMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosyl-ATP diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Histidine biosynthesis bifunctional protein hisIE HAMAP MF_01019		PRO_0000136444
Regions
	Region	1 – 115	115	Phosphoribosyl-AMP cyclohydrolase HAMAP MF_01019
	Region	116 – 205	90	Phosphoribosyl-ATP pyrophosphohydrolase HAMAP MF_01019

Sequences

	Sequence		Length	Mass (Da)
	Q8R886-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E2DAFD6A8C5BD65A	FASTA	205	23,864
	10 20 30 40 50 60 MIDIKELKFD EKGLIPAIVQ DYYTKQVLML AYMNEESLKL TLEKGETYFF SRSRGKIWHK 70 80 90 100 110 120 GETSGNTQKV KKIFYDCDED ALLIQVEAKG PACHTGNISC FYRSFDEETE DGIEILNKLY 130 140 150 160 170 180 ERIKGRKINP VEGSYTNYLF EKGIDKILKK VGEEATEVVI ASKNDSKDEI VYEVSDLIYH 190 200 LMVLLVEKNI TLNDIYNELE RRYKK

References

[1]

"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed: 11997336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008691 Genomic DNA. Translation: AAM25297.1.
RefSeq	NP_623693.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	997442.
GenomeReviews	Gene locus TTE2132 in contig AE008691_GR.
KEGG	tte:TTE2132.
NMPDR	fig|273068.3.peg.2236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8R886.
OMA	VHYWSRS.
Enzyme and pathway databases
BioCyc	TTEN273068:TTE2132-MON.
BRENDA	3.5.4.19. 276887. 3.6.1.31. 276887.
Family and domain databases
HAMAP	MF_01019. [Tree]
InterPro	IPR002496. PRA_CycHdrlase. IPR008179. PRib-ATP_pyrophosphohydrolase. [Graphical view]
Pfam	PF01502. PRA-CH. 1 hit. PF01503. PRA-PH. 1 hit. [Graphical view]
ProDom	PD002610. PRA_cyclohydro. 1 hit. PD002611. Pra_PH/CH. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03188. histidine_hisI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS2_THETN

Accession

Primary (citable) accession number: Q8R886

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)