Q8R7T1 (SYE2_THETN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	TTE2317

Organism

Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]

Taxonomic identifier

273068 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Caldanaerobacter › Caldanaerobacter subterraneus › Caldanaerobacter subterraneus subsp. tengcongensis

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Glutamate--tRNA ligase 2 HAMAP-Rule MF_00022		PRO_0000119685
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	252 – 256	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8R7T1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 3B71400ACB925E39	FASTA	485	56,824
	10 20 30 40 50 60 MKEFRVRFAP SPTGPIHIGN MRTALFNYLF SRSEGATFVL RIEDTDLERS SKGFEELIFK 70 80 90 100 110 120 ELKWLGIEWD EGPDKPGPYG PYRQSERLEI YHRFAQKLIE EKKAYRCYCT PEELEEDRKK 130 140 150 160 170 180 AIERGEIPRY SGRCRYLTKE QEETFIREGR KPVIRFMVPD DEVIEFEDMI KGKITIKSDT 190 200 210 220 230 240 LGGDMVIIKS DGMPTYNFAV VIDDALMKIT HVIRGEDHIY NTPKQILIYK ALGFEIPKFA 250 260 270 280 290 300 HVPLILGPDR TKLSKRHGHT YIGQYRELGY LPEAMFNFLS LLSWYPEDNV ELMSKEEIIR 310 320 330 340 350 360 KFNFKRIHKS NPVFDIEKLN WMNQQYIQKS PVERIVDLAI PHLKKAGYID EIDELRYNWL 370 380 390 400 410 420 KDVISLYKDG LSYVAQIVDM AKTFFVEEVD YTGEMIEFLK SPNSIKVLEA FKGYLKDKSE 430 440 450 460 470 480 ITEDDVREWM KKAQKELGVK GKEFFMPIRI AVTGEEHGPE LVKVLALLGK NRVVKRLDRV LNLIE

References

[1]

"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008691 Genomic DNA. Translation: AAM25458.1.
RefSeq	NP_623854.1. NC_003869.1.
3D structure databases
ProteinModelPortal	Q8R7T1.
ModBase	Search...
Protein-protein interaction databases
STRING	273068.TTE2317.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM25458; AAM25458; TTE2317.
GeneID	996607.
KEGG	tte:TTE2317.
PATRIC	23899367. VBITheTen82880_2306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252720.
KO	K09698.
OMA	ISFESDG.
ProtClustDB	PRK01406.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_THETN

Accession

Primary (citable) accession number: Q8R7T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program