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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 406ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSUB273068:GJEB-4319-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:TTE2395
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467tRNA(Ile)-lysidine synthasePRO_0000181794Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273068.TTE2395.

Structurei

3D structure databases

ProteinModelPortaliQ8R7K9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
KOiK04075.
OMAiNDISYPL.
OrthoDBiPOG091H02FP.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R7K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMSFQRFGM IEKVLRTIEK YNMIEKDDKI VMGISGGPDS LCMLDVLFNL
60 70 80 90 100
KGKFNLKLYV VHVNHMIRGE DAKKDAEFVE KLCKDLDLPF FLFEENIPYL
110 120 130 140 150
AKKMGLSEEQ AGRYVRYKAF EETLKRVGGN KIAVAHNKND VAETVLLNIL
160 170 180 190 200
RGTGLRGLIG IKPVNGNIIR PLIEIERREI EKYLKDKNLH PRIDHTNYED
210 220 230 240 250
LYTRNKIRLK VIPYIEEVFK IDLVENLSRM AAILLEEDDY LEAKCEEVFN
260 270 280 290 300
QICEINGEEI KVDVDALKSQ HTAIKRRLVR RMYFYVKGET DGLEYGHVED
310 320 330 340 350
VLNLLDKPTS SKIDLPFEIE ALKMYNNLVI RKKKTKEKVK FKEVLKIPGV
360 370 380 390 400
TTIEGIGKFK AYVVDISQVD DFNKGEYIKF FDYDKIKGEI VVKSREDGDR
410 420 430 440 450
FSPLGMRGTK KLKEFFIDEK IPREERDYIP LVAIGKEIVW VVGYRMSEKF
460
KVDKNTSKVL VIEYTKE
Length:467
Mass (Da):54,355
Last modified:June 1, 2002 - v1
Checksum:i7A054CA35E698E3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM25534.1.

Genome annotation databases

EnsemblBacteriaiAAM25534; AAM25534; TTE2395.
KEGGitte:TTE2395.
PATRICi23899525. VBITheTen82880_2385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA. Translation: AAM25534.1.

3D structure databases

ProteinModelPortaliQ8R7K9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273068.TTE2395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM25534; AAM25534; TTE2395.
KEGGitte:TTE2395.
PATRICi23899525. VBITheTen82880_2385.

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
KOiK04075.
OMAiNDISYPL.
OrthoDBiPOG091H02FP.

Enzyme and pathway databases

BioCyciCSUB273068:GJEB-4319-MONOMER.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_CALS4
AccessioniPrimary (citable) accession number: Q8R7K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2002
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.