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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 40ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:TTE2395
OrganismiCaldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Taxonomic identifieri273068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter
Proteomesi
  • UP000000555 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001817941 – 467tRNA(Ile)-lysidine synthaseAdd BLAST467

Interactioni

Protein-protein interaction databases

STRINGi273068.TTE2395

Structurei

3D structure databases

ProteinModelPortaliQ8R7K9
SMRiQ8R7K9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U Bacteria
COG0037 LUCA
HOGENOMiHOG000236506
KOiK04075
OMAiAHCNFNL
OrthoDBiPOG091H02FP

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR012796 Lysidine-tRNA-synth_C
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
PANTHERiPTHR43033:SF1 PTHR43033:SF1, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF11734 TilS_C, 1 hit
SMARTiView protein in SMART
SM00977 TilS_C, 1 hit
TIGRFAMsiTIGR02433 lysidine_TilS_C, 1 hit
TIGR02432 lysidine_TilS_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R7K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMSFQRFGM IEKVLRTIEK YNMIEKDDKI VMGISGGPDS LCMLDVLFNL
60 70 80 90 100
KGKFNLKLYV VHVNHMIRGE DAKKDAEFVE KLCKDLDLPF FLFEENIPYL
110 120 130 140 150
AKKMGLSEEQ AGRYVRYKAF EETLKRVGGN KIAVAHNKND VAETVLLNIL
160 170 180 190 200
RGTGLRGLIG IKPVNGNIIR PLIEIERREI EKYLKDKNLH PRIDHTNYED
210 220 230 240 250
LYTRNKIRLK VIPYIEEVFK IDLVENLSRM AAILLEEDDY LEAKCEEVFN
260 270 280 290 300
QICEINGEEI KVDVDALKSQ HTAIKRRLVR RMYFYVKGET DGLEYGHVED
310 320 330 340 350
VLNLLDKPTS SKIDLPFEIE ALKMYNNLVI RKKKTKEKVK FKEVLKIPGV
360 370 380 390 400
TTIEGIGKFK AYVVDISQVD DFNKGEYIKF FDYDKIKGEI VVKSREDGDR
410 420 430 440 450
FSPLGMRGTK KLKEFFIDEK IPREERDYIP LVAIGKEIVW VVGYRMSEKF
460
KVDKNTSKVL VIEYTKE
Length:467
Mass (Da):54,355
Last modified:June 1, 2002 - v1
Checksum:i7A054CA35E698E3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008691 Genomic DNA Translation: AAM25534.1

Genome annotation databases

EnsemblBacteriaiAAM25534; AAM25534; TTE2395
KEGGitte:TTE2395

Similar proteinsi

Entry informationi

Entry nameiTILS_CALS4
AccessioniPrimary (citable) accession number: Q8R7K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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