Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8R778 (DDL_THETN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:TTE2545
OrganismThermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP]
Taxonomic identifier273068 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCaldanaerobacter

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177897

Regions

Domain148 – 358211ATP-grasp
Nucleotide binding181 – 23656ATP By similarity

Sites

Metal binding3151Magnesium or manganese 1 By similarity
Metal binding3291Magnesium or manganese 1 By similarity
Metal binding3291Magnesium or manganese 2 By similarity
Metal binding3311Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R778 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8DE768C15907B529

FASTA38944,676
        10         20         30         40         50         60 
MLKVAVMFGG KSVEHEVSII TGLQVIENMN KEKYEPIPVY VGRDGRWYTG KGLLDISNYK 

        70         80         90        100        110        120 
DIPSLLKKCE QVVLPPVPGL NKLYYYPFRK KFMSKEYDYI DVDVIFPAFH GTHGEDGTMQ 

       130        140        150        160        170        180 
GLFQLANIPY VGSGVVGSAT GMDKVIMKDI FKANNLPIVN YLWFLRKEYE KNKEAMLDLI 

       190        200        210        220        230        240 
ESKLKYPMFV KPANLGSSIG ISKAKNRDEL FDAIEIAIYY DRKIIVEEAV KDPMEVNCSV 

       250        260        270        280        290        300 
MGIDRDLEIS LCEMPVPWEE FLSYQDKYMR KEKGGKGSTT RQIPAPIPEE KTKEIQELAK 

       310        320        330        340        350        360 
KVFQILDCSG LARIDFLLEK ETMKVYVNEI NTIPGSFAFY LWKPLGISFE KLIDRLIEYA 

       370        380 
VERHKEYARN MYTFETTLLL KAADRGTKM 

« Hide

References

[1]"A complete sequence of the T. tengcongensis genome."
Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. expand/collapse author list , Wang J., Yu J., Yang H.
Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008691 Genomic DNA. Translation: AAM25670.1.
RefSeqNP_624066.1. NC_003869.1.

3D structure databases

ProteinModelPortalQ8R778.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273068.TTE2545.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM25670; AAM25670; TTE2545.
GeneID997660.
KEGGtte:TTE2545.
PATRIC23899847. VBITheTen82880_2542.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAGIATPRF.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_THETN
AccessionPrimary (citable) accession number: Q8R778
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways