Q8R777 (Q8R777_THETN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase RuleBase RU004136
EC=6.3.2.10 RuleBase RU004136

Gene names

Name:	MurF EMBL AAM25671.1
Ordered Locus Names:	TTE2546

Organism

Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis) [Complete proteome] [HAMAP] EMBL AAM25671.1

Taxonomic identifier

273068 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Caldanaerobacter › Caldanaerobacter subterraneus › Caldanaerobacter subterraneus subsp. tengcongensis

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurCDEF family. RuleBase RU003630

Ontologies

Keywords
Biological process	Cell cycle Cell division RuleBase RU004136 Cell shape Cell wall biogenesis/degradation RuleBase RU004136 Peptidoglycan synthesis RuleBase RU004136
Ligand	ATP-binding RuleBase RU003630 Nucleotide-binding
Molecular function	Ligase RuleBase RU003630
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: EC UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8R777 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 693778F407F621D4	FASTA	508	57,601
	10 20 30 40 50 60 MAQLEGYKIK NFFKWHFRNY DRWVKESLLA LLSAGIAFIM NIFRLPSEII ILVPIALFFY 70 80 90 100 110 120 ITYKEVTTKK KAKKPLVFTA RATRLFLTFW LLIALSFYFL KKNFQAPEVF SAEVLILTAL 130 140 150 160 170 180 LPVYMALSLV ITFPLEKFIQ FLYYKSAQRK IRNRKDLITI GITGSYGKTS TKHFITAVLS 190 200 210 220 230 240 EKFSVLMTPE SYNTPMGVTK VIRNELKDTH QIFVCEMGAR QRGDIKVLVK LANPKIGVIT 250 260 270 280 290 300 SIGPQHLETF GTIENVAKTK FELVEGLPED GIAVLNGDNY YCRTFGEKFA VRKLFYGTES 310 320 330 340 350 360 PIERKYFLWA ENIINTRQGL KFDAVTEDGM RIKCEVPLLG RHNVSNLLAA ISVALALGLN 370 380 390 400 410 420 EEEIKRGLSR IEPVPHRLQL ISTPHGVTVI DDAFNSNPEG AKEALACLKE MDAKRRIIVT 430 440 450 460 470 480 PGMVELGEKE YEENKNLGKN IAEVCDYAIL VGKKRSRPIF EGLKEASFPE EKVFVVANLD 490 500 EATKVLQTLL QPGDVVLFEN DLPDNYEG

References

[1]

"A complete sequence of the T. tengcongensis genome." Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R. , Wang J., Yu J., Yang H. Genome Res. 12:689-700(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008691 Genomic DNA. Translation: AAM25671.1.
RefSeq	NP_624067.1. NC_003869.1.
3D structure databases
ProteinModelPortal	Q8R777.
ModBase	Search...
Protein-protein interaction databases
STRING	273068.TTE2546.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM25671; AAM25671; TTE2546.
GeneID	996190.
KEGG	tte:TTE2546.
PATRIC	23899849. VBITheTen82880_2543.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000292178.
KO	K01929.
OMA	FANDAPN.
ProtClustDB	CLSK812549.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.40.1190.10. 1 hit. 3.90.190.20. 1 hit.
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005863. UDP-N-AcMur-pentapeptide_synth. [Graphical view]
PANTHER	PTHR23135:SF3. PTHR23135:SF3. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q8R777_THETN

Accession

Primary (citable) accession number: Q8R777

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)