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Q8R6G8

- TRMB_FUSNN

UniProt

Q8R6G8 - TRMB_FUSNN

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Protein

Bifunctional glycosyltransferase/methyltransferase

Gene

trmB

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei470 – 4701S-adenosyl-L-methionineBy similarity
Binding sitei495 – 4951S-adenosyl-L-methionineBy similarity
Active sitei544 – 5441By similarity
Binding sitei544 – 5441S-adenosyl-L-methionineBy similarity
Binding sitei578 – 5781SubstrateBy similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glycosyltransferase/methyltransferase
Including the following 2 domains:
KdtA protein homolog (EC:2.-.-.-)
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:FN1606
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
ProteomesiUP000002521: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 640640Bifunctional glycosyltransferase/methyltransferasePRO_0000171430Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190304.FN1606.

Structurei

3D structure databases

ProteinModelPortaliQ8R6G8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 438438GlycosyltransferaseAdd
BLAST
Regioni439 – 640202tRNA (guanine-N(7)-)-methyltransferaseAdd
BLAST
Regioni617 – 6204Substrate bindingBy similarity

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiCOG0220.
InParanoidiQ8R6G8.
KOiK02527.
K03439.
OMAiMASTHCE.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR007507. Glycos_transf_N.
IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF04413. Glycos_transf_N. 1 hit.
PF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R6G8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNLLRKIAL TLYRPFMKEK MKTFINKRLS QDFSDLKDEE YIWIHCSSVG
60 70 80 90 100
EVNLSEDLVK KFYSISRKNI LISVFTDTGY ENAVKKYSDK KKIKVIYFPV
110 120 130 140 150
DDKKKINEIL NKIKLKLLVL VETELWPNLI NEVNEKNSRI IVVNGRISDR
160 170 180 190 200
SYPRYKKLKF LLKSMLQKIA FFYMQSEIDK ERIVSLGAIK EKVENVGNLK
210 220 230 240 250
FSISLEKYSD IEKKEYRKFL NIGDRKVFVA GSTRTGEDEI ILDVFKRLKN
260 270 280 290 300
YVLIIVPRHL DRLPKIENLI KENNLTYVKY SDLENNTSTG KENIILVDKM
310 320 330 340 350
GVLRKLYSIS DIAFVGGTLV NIGGHNLLEP LFYRKTVIFG KYTQNVVDIA
360 370 380 390 400
KEILRRKIGF QVENVEEFVK AIETIENEKN SDEEINSFFE ENRLIALNIV
410 420 430 440 450
KKENLIMNNI KEEAKDLWKH FFHSEKSNYN MYMYKLLDYP EYIMYDNDVM
460 470 480 490 500
KEKKSKWSEY FGNSDQIAVE IGTGSGNFIY QLAERNPNKN FIGLELRFKR
510 520 530 540 550
LVLAAQKCKK RNIKNVAFLR KRGEELEDFL ANNEISEMYI NFPDPWEGTE
560 570 580 590 600
KNRIIQERLF KTLDKIMKKD GMLYFKTDHD VYYNDVLELV KTLDNYEVIY
610 620 630 640
HTSDLHNSEK AENNIKTEFE QLFLHKHNKN INYIEIKKIV
Length:640
Mass (Da):75,760
Last modified:June 1, 2002 - v1
Checksum:i6745564DC10BFE6E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009951 Genomic DNA. Translation: AAL93721.1.
RefSeqiNP_602422.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL93721; AAL93721; FN1606.
GeneIDi992776.
KEGGifnu:FN1606.
PATRICi21948735. VBIFusNuc122357_0098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009951 Genomic DNA. Translation: AAL93721.1 .
RefSeqi NP_602422.1. NC_003454.1.

3D structure databases

ProteinModelPortali Q8R6G8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190304.FN1606.

Protein family/group databases

CAZyi GT30. Glycosyltransferase Family 30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL93721 ; AAL93721 ; FN1606 .
GeneIDi 992776.
KEGGi fnu:FN1606.
PATRICi 21948735. VBIFusNuc122357_0098.

Phylogenomic databases

eggNOGi COG0220.
InParanoidi Q8R6G8.
KOi K02527.
K03439.
OMAi MASTHCE.
OrthoDBi EOG6K6VBC.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR007507. Glycos_transf_N.
IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF04413. Glycos_transf_N. 1 hit.
PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Entry informationi

Entry nameiTRMB_FUSNN
AccessioniPrimary (citable) accession number: Q8R6G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3