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Protein

Bifunctional glycosyltransferase/methyltransferase

Gene

trmB

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei470S-adenosyl-L-methionineBy similarity1
Binding sitei495S-adenosyl-L-methionineBy similarity1
Active sitei544By similarity1
Binding sitei544S-adenosyl-L-methionineBy similarity1
Binding sitei578SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciFNUC190304:G1FZS-109-MONOMER

Protein family/group databases

CAZyiGT30 Glycosyltransferase Family 30

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glycosyltransferase/methyltransferase
Including the following 2 domains:
KdtA protein homolog (EC:2.-.-.-)
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:FN1606
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
Proteomesi
  • UP000002521 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714301 – 640Bifunctional glycosyltransferase/methyltransferaseAdd BLAST640

Proteomic databases

PRIDEiQ8R6G8

Interactioni

Protein-protein interaction databases

STRINGi190304.FN1606

Structurei

3D structure databases

ProteinModelPortaliQ8R6G8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 438GlycosyltransferaseAdd BLAST438
Regioni439 – 640tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST202
Regioni617 – 620Substrate bindingBy similarity4

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105D8A Bacteria
COG0220 LUCA
COG1519 LUCA
InParanoidiQ8R6G8
KOiK02527
K03439

Family and domain databases

Gene3Di3.40.50.11720, 1 hit
HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR007507 Glycos_transf_N
IPR038107 Glycos_transf_N_sf
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PfamiView protein in Pfam
PF04413 Glycos_transf_N, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R6G8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNLLRKIAL TLYRPFMKEK MKTFINKRLS QDFSDLKDEE YIWIHCSSVG
60 70 80 90 100
EVNLSEDLVK KFYSISRKNI LISVFTDTGY ENAVKKYSDK KKIKVIYFPV
110 120 130 140 150
DDKKKINEIL NKIKLKLLVL VETELWPNLI NEVNEKNSRI IVVNGRISDR
160 170 180 190 200
SYPRYKKLKF LLKSMLQKIA FFYMQSEIDK ERIVSLGAIK EKVENVGNLK
210 220 230 240 250
FSISLEKYSD IEKKEYRKFL NIGDRKVFVA GSTRTGEDEI ILDVFKRLKN
260 270 280 290 300
YVLIIVPRHL DRLPKIENLI KENNLTYVKY SDLENNTSTG KENIILVDKM
310 320 330 340 350
GVLRKLYSIS DIAFVGGTLV NIGGHNLLEP LFYRKTVIFG KYTQNVVDIA
360 370 380 390 400
KEILRRKIGF QVENVEEFVK AIETIENEKN SDEEINSFFE ENRLIALNIV
410 420 430 440 450
KKENLIMNNI KEEAKDLWKH FFHSEKSNYN MYMYKLLDYP EYIMYDNDVM
460 470 480 490 500
KEKKSKWSEY FGNSDQIAVE IGTGSGNFIY QLAERNPNKN FIGLELRFKR
510 520 530 540 550
LVLAAQKCKK RNIKNVAFLR KRGEELEDFL ANNEISEMYI NFPDPWEGTE
560 570 580 590 600
KNRIIQERLF KTLDKIMKKD GMLYFKTDHD VYYNDVLELV KTLDNYEVIY
610 620 630 640
HTSDLHNSEK AENNIKTEFE QLFLHKHNKN INYIEIKKIV
Length:640
Mass (Da):75,760
Last modified:June 1, 2002 - v1
Checksum:i6745564DC10BFE6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA Translation: AAL93721.1
RefSeqiNP_602422.1, NC_003454.1
WP_011015702.1, NC_003454.1

Genome annotation databases

EnsemblBacteriaiAAL93721; AAL93721; FN1606
GeneIDi992776
KEGGifnu:FN1606
PATRICifig|190304.8.peg.98

Similar proteinsi

Entry informationi

Entry nameiTRMB_FUSNN
AccessioniPrimary (citable) accession number: Q8R6G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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