Q8R6G8 (TRMB_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional glycosyltransferase/methyltransferase

Including the following 2 domains:

1. KdtA protein homolog
   EC=2.-.-.-
2. tRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.33
   Alternative name(s):
   tRNA (guanine(46)-N(7))-methyltransferase
   tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	FN1606

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fusobacteria › Fusobacteriia › Fusobacteriales › Fusobacteriaceae › Fusobacterium › Fusobacterium nucleatum › Fusobacterium nucleatum subsp. nucleatum

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 1 family. In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Bifunctional glycosyltransferase/methyltransferase HAMAP-Rule MF_01057		PRO_0000171430
Regions
	Region	1 – 438	438	Glycosyltransferase HAMAP-Rule MF_01057
	Region	439 – 640	202	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
	Region	617 – 620	4	Substrate binding Potential
Sites
	Binding site	470	1	S-adenosyl-L-methionine By similarity
	Binding site	495	1	S-adenosyl-L-methionine By similarity
	Binding site	544	1	S-adenosyl-L-methionine By similarity
	Binding site	578	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8R6G8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 6745564DC10BFE6E	FASTA	640	75,760
	10 20 30 40 50 60 MYNLLRKIAL TLYRPFMKEK MKTFINKRLS QDFSDLKDEE YIWIHCSSVG EVNLSEDLVK 70 80 90 100 110 120 KFYSISRKNI LISVFTDTGY ENAVKKYSDK KKIKVIYFPV DDKKKINEIL NKIKLKLLVL 130 140 150 160 170 180 VETELWPNLI NEVNEKNSRI IVVNGRISDR SYPRYKKLKF LLKSMLQKIA FFYMQSEIDK 190 200 210 220 230 240 ERIVSLGAIK EKVENVGNLK FSISLEKYSD IEKKEYRKFL NIGDRKVFVA GSTRTGEDEI 250 260 270 280 290 300 ILDVFKRLKN YVLIIVPRHL DRLPKIENLI KENNLTYVKY SDLENNTSTG KENIILVDKM 310 320 330 340 350 360 GVLRKLYSIS DIAFVGGTLV NIGGHNLLEP LFYRKTVIFG KYTQNVVDIA KEILRRKIGF 370 380 390 400 410 420 QVENVEEFVK AIETIENEKN SDEEINSFFE ENRLIALNIV KKENLIMNNI KEEAKDLWKH 430 440 450 460 470 480 FFHSEKSNYN MYMYKLLDYP EYIMYDNDVM KEKKSKWSEY FGNSDQIAVE IGTGSGNFIY 490 500 510 520 530 540 QLAERNPNKN FIGLELRFKR LVLAAQKCKK RNIKNVAFLR KRGEELEDFL ANNEISEMYI 550 560 570 580 590 600 NFPDPWEGTE KNRIIQERLF KTLDKIMKKD GMLYFKTDHD VYYNDVLELV KTLDNYEVIY 610 620 630 640 HTSDLHNSEK AENNIKTEFE QLFLHKHNKN INYIEIKKIV

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL93721.1.
RefSeq	NP_602422.1. NC_003454.1.
3D structure databases
ProteinModelPortal	Q8R6G8.
ModBase	Search...
Protein-protein interaction databases
STRING	190304.FN1606.
Protein family/group databases
CAZy	GT30. Glycosyltransferase Family 30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL93721; AAL93721; FN1606.
GeneID	992776.
KEGG	fnu:FN1606.
PATRIC	21948735. VBIFusNuc122357_0098.
Phylogenomic databases
eggNOG	COG0220.
KO	K00599. K02527.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB. Fused.
InterPro	IPR007507. Glycos_transf_N. IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF04413. Glycos_transf_N. 1 hit. PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_FUSNN

Accession

Primary (citable) accession number: Q8R6G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program