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Q8R6G8

- TRMB_FUSNN

UniProt

Q8R6G8 - TRMB_FUSNN

Protein

Bifunctional glycosyltransferase/methyltransferase

Gene

trmB

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei470 – 4701S-adenosyl-L-methionineBy similarity
    Binding sitei495 – 4951S-adenosyl-L-methionineBy similarity
    Active sitei544 – 5441By similarity
    Binding sitei544 – 5441S-adenosyl-L-methionineBy similarity
    Binding sitei578 – 5781SubstrateBy similarity

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    CAZyiGT30. Glycosyltransferase Family 30.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional glycosyltransferase/methyltransferase
    Including the following 2 domains:
    KdtA protein homolog (EC:2.-.-.-)
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:FN1606
    OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
    Taxonomic identifieri190304 [NCBI]
    Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
    ProteomesiUP000002521: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 640640Bifunctional glycosyltransferase/methyltransferasePRO_0000171430Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi190304.FN1606.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R6G8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 438438GlycosyltransferaseAdd
    BLAST
    Regioni439 – 640202tRNA (guanine-N(7)-)-methyltransferaseAdd
    BLAST
    Regioni617 – 6204Substrate bindingBy similarity

    Sequence similaritiesi

    In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
    In the N-terminal section; belongs to the glycosyltransferase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK00599.
    K02527.
    OMAiMASTHCE.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR007507. Glycos_transf_N.
    IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF04413. Glycos_transf_N. 1 hit.
    PF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R6G8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYNLLRKIAL TLYRPFMKEK MKTFINKRLS QDFSDLKDEE YIWIHCSSVG    50
    EVNLSEDLVK KFYSISRKNI LISVFTDTGY ENAVKKYSDK KKIKVIYFPV 100
    DDKKKINEIL NKIKLKLLVL VETELWPNLI NEVNEKNSRI IVVNGRISDR 150
    SYPRYKKLKF LLKSMLQKIA FFYMQSEIDK ERIVSLGAIK EKVENVGNLK 200
    FSISLEKYSD IEKKEYRKFL NIGDRKVFVA GSTRTGEDEI ILDVFKRLKN 250
    YVLIIVPRHL DRLPKIENLI KENNLTYVKY SDLENNTSTG KENIILVDKM 300
    GVLRKLYSIS DIAFVGGTLV NIGGHNLLEP LFYRKTVIFG KYTQNVVDIA 350
    KEILRRKIGF QVENVEEFVK AIETIENEKN SDEEINSFFE ENRLIALNIV 400
    KKENLIMNNI KEEAKDLWKH FFHSEKSNYN MYMYKLLDYP EYIMYDNDVM 450
    KEKKSKWSEY FGNSDQIAVE IGTGSGNFIY QLAERNPNKN FIGLELRFKR 500
    LVLAAQKCKK RNIKNVAFLR KRGEELEDFL ANNEISEMYI NFPDPWEGTE 550
    KNRIIQERLF KTLDKIMKKD GMLYFKTDHD VYYNDVLELV KTLDNYEVIY 600
    HTSDLHNSEK AENNIKTEFE QLFLHKHNKN INYIEIKKIV 640
    Length:640
    Mass (Da):75,760
    Last modified:June 1, 2002 - v1
    Checksum:i6745564DC10BFE6E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009951 Genomic DNA. Translation: AAL93721.1.
    RefSeqiNP_602422.1. NC_003454.1.
    WP_011015702.1. NC_003454.1.

    Genome annotation databases

    EnsemblBacteriaiAAL93721; AAL93721; FN1606.
    GeneIDi992776.
    KEGGifnu:FN1606.
    PATRICi21948735. VBIFusNuc122357_0098.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009951 Genomic DNA. Translation: AAL93721.1 .
    RefSeqi NP_602422.1. NC_003454.1.
    WP_011015702.1. NC_003454.1.

    3D structure databases

    ProteinModelPortali Q8R6G8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 190304.FN1606.

    Protein family/group databases

    CAZyi GT30. Glycosyltransferase Family 30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL93721 ; AAL93721 ; FN1606 .
    GeneIDi 992776.
    KEGGi fnu:FN1606.
    PATRICi 21948735. VBIFusNuc122357_0098.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K00599.
    K02527.
    OMAi MASTHCE.
    OrthoDBi EOG6K6VBC.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR007507. Glycos_transf_N.
    IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF04413. Glycos_transf_N. 1 hit.
    PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

    Entry informationi

    Entry nameiTRMB_FUSNN
    AccessioniPrimary (citable) accession number: Q8R6G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3