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Protein

Bifunctional glycosyltransferase/methyltransferase

Gene

trmB

Organism
Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei470S-adenosyl-L-methionineBy similarity1
Binding sitei495S-adenosyl-L-methionineBy similarity1
Active sitei544By similarity1
Binding sitei544S-adenosyl-L-methionineBy similarity1
Binding sitei578SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glycosyltransferase/methyltransferase
Including the following 2 domains:
KdtA protein homolog (EC:2.-.-.-)
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:FN1606
OrganismiFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
Taxonomic identifieri190304 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium
Proteomesi
  • UP000002521 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714301 – 640Bifunctional glycosyltransferase/methyltransferaseAdd BLAST640

Interactioni

Protein-protein interaction databases

STRINGi190304.FN1606.

Structurei

3D structure databases

ProteinModelPortaliQ8R6G8.
SMRiQ8R6G8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 438GlycosyltransferaseAdd BLAST438
Regioni439 – 640tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST202
Regioni617 – 620Substrate bindingBy similarity4

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the glycosyltransferase 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105D8A. Bacteria.
COG0220. LUCA.
COG1519. LUCA.
InParanoidiQ8R6G8.
KOiK02527.
K03439.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR007507. Glycos_transf_N.
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PfamiView protein in Pfam
PF04413. Glycos_transf_N. 1 hit.
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8R6G8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYNLLRKIAL TLYRPFMKEK MKTFINKRLS QDFSDLKDEE YIWIHCSSVG
60 70 80 90 100
EVNLSEDLVK KFYSISRKNI LISVFTDTGY ENAVKKYSDK KKIKVIYFPV
110 120 130 140 150
DDKKKINEIL NKIKLKLLVL VETELWPNLI NEVNEKNSRI IVVNGRISDR
160 170 180 190 200
SYPRYKKLKF LLKSMLQKIA FFYMQSEIDK ERIVSLGAIK EKVENVGNLK
210 220 230 240 250
FSISLEKYSD IEKKEYRKFL NIGDRKVFVA GSTRTGEDEI ILDVFKRLKN
260 270 280 290 300
YVLIIVPRHL DRLPKIENLI KENNLTYVKY SDLENNTSTG KENIILVDKM
310 320 330 340 350
GVLRKLYSIS DIAFVGGTLV NIGGHNLLEP LFYRKTVIFG KYTQNVVDIA
360 370 380 390 400
KEILRRKIGF QVENVEEFVK AIETIENEKN SDEEINSFFE ENRLIALNIV
410 420 430 440 450
KKENLIMNNI KEEAKDLWKH FFHSEKSNYN MYMYKLLDYP EYIMYDNDVM
460 470 480 490 500
KEKKSKWSEY FGNSDQIAVE IGTGSGNFIY QLAERNPNKN FIGLELRFKR
510 520 530 540 550
LVLAAQKCKK RNIKNVAFLR KRGEELEDFL ANNEISEMYI NFPDPWEGTE
560 570 580 590 600
KNRIIQERLF KTLDKIMKKD GMLYFKTDHD VYYNDVLELV KTLDNYEVIY
610 620 630 640
HTSDLHNSEK AENNIKTEFE QLFLHKHNKN INYIEIKKIV
Length:640
Mass (Da):75,760
Last modified:June 1, 2002 - v1
Checksum:i6745564DC10BFE6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009951 Genomic DNA. Translation: AAL93721.1.
RefSeqiNP_602422.1. NC_003454.1.
WP_011015702.1. NC_003454.1.

Genome annotation databases

EnsemblBacteriaiAAL93721; AAL93721; FN1606.
GeneIDi992776.
KEGGifnu:FN1606.
PATRICifig|190304.8.peg.98.

Similar proteinsi

Entry informationi

Entry nameiTRMB_FUSNN
AccessioniPrimary (citable) accession number: Q8R6G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2002
Last modified: August 30, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families