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Q8R6G8 (TRMB_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional glycosyltransferase/methyltransferase

Including the following 2 domains:

  1. KdtA protein homolog
    EC=2.-.-.-
  2. tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:FN1606
OrganismFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131) [Reference proteome] [HAMAP]
Taxonomic identifier190304 [NCBI]
Taxonomic lineageBacteriaFusobacteriaFusobacterialesFusobacteriaceaeFusobacterium

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Sequence similarities

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

In the N-terminal section; belongs to the glycosyltransferase 1 family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640Bifunctional glycosyltransferase/methyltransferase HAMAP-Rule MF_01057
PRO_0000171430

Regions

Region1 – 438438Glycosyltransferase HAMAP-Rule MF_01057
Region439 – 640202tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
Region617 – 6204Substrate binding By similarity

Sites

Active site5441 By similarity
Binding site4701S-adenosyl-L-methionine By similarity
Binding site4951S-adenosyl-L-methionine By similarity
Binding site5441S-adenosyl-L-methionine By similarity
Binding site5781Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R6G8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 6745564DC10BFE6E

FASTA64075,760
        10         20         30         40         50         60 
MYNLLRKIAL TLYRPFMKEK MKTFINKRLS QDFSDLKDEE YIWIHCSSVG EVNLSEDLVK 

        70         80         90        100        110        120 
KFYSISRKNI LISVFTDTGY ENAVKKYSDK KKIKVIYFPV DDKKKINEIL NKIKLKLLVL 

       130        140        150        160        170        180 
VETELWPNLI NEVNEKNSRI IVVNGRISDR SYPRYKKLKF LLKSMLQKIA FFYMQSEIDK 

       190        200        210        220        230        240 
ERIVSLGAIK EKVENVGNLK FSISLEKYSD IEKKEYRKFL NIGDRKVFVA GSTRTGEDEI 

       250        260        270        280        290        300 
ILDVFKRLKN YVLIIVPRHL DRLPKIENLI KENNLTYVKY SDLENNTSTG KENIILVDKM 

       310        320        330        340        350        360 
GVLRKLYSIS DIAFVGGTLV NIGGHNLLEP LFYRKTVIFG KYTQNVVDIA KEILRRKIGF 

       370        380        390        400        410        420 
QVENVEEFVK AIETIENEKN SDEEINSFFE ENRLIALNIV KKENLIMNNI KEEAKDLWKH 

       430        440        450        460        470        480 
FFHSEKSNYN MYMYKLLDYP EYIMYDNDVM KEKKSKWSEY FGNSDQIAVE IGTGSGNFIY 

       490        500        510        520        530        540 
QLAERNPNKN FIGLELRFKR LVLAAQKCKK RNIKNVAFLR KRGEELEDFL ANNEISEMYI 

       550        560        570        580        590        600 
NFPDPWEGTE KNRIIQERLF KTLDKIMKKD GMLYFKTDHD VYYNDVLELV KTLDNYEVIY 

       610        620        630        640 
HTSDLHNSEK AENNIKTEFE QLFLHKHNKN INYIEIKKIV 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009951 Genomic DNA. Translation: AAL93721.1.
RefSeqNP_602422.1. NC_003454.1.

3D structure databases

ProteinModelPortalQ8R6G8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190304.FN1606.

Protein family/group databases

CAZyGT30. Glycosyltransferase Family 30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL93721; AAL93721; FN1606.
GeneID992776.
KEGGfnu:FN1606.
PATRIC21948735. VBIFusNuc122357_0098.

Phylogenomic databases

eggNOGCOG0220.
KOK00599.
K02527.
OrthoDBEOG6K6VBC.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR007507. Glycos_transf_N.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF04413. Glycos_transf_N. 1 hit.
PF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_FUSNN
AccessionPrimary (citable) accession number: Q8R6G8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families