Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cell adhesion molecule 1

Gene

Cadm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates homophilic cell-cell adhesion in a Ca2+-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca2+-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.10 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: GO_Central
  • PDZ domain binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • signaling receptor binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • bone development Source: MGI
  • calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: MGI
  • cell adhesion Source: MGI
  • cell differentiation Source: UniProtKB-KW
  • cell recognition Source: UniProtKB
  • detection of stimulus Source: UniProtKB
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • immune system process Source: UniProtKB-KW
  • positive regulation of cytokine secretion Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity Source: UniProtKB
  • spermatogenesis Source: UniProtKB-KW
  • susceptibility to natural killer cell mediated cytotoxicity Source: UniProtKB
  • synapse assembly Source: MGI
  • unidimensional cell growth Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processApoptosis, Cell adhesion, Differentiation, Immunity, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-MMU-418990 Adherens junctions interactions
R-MMU-420597 Nectin/Necl trans heterodimerization

Names & Taxonomyi

Protein namesi
Recommended name:
Cell adhesion molecule 1
Alternative name(s):
Immunoglobulin superfamily member 4
Short name:
IgSF4
Nectin-like protein 2
Short name:
NECL-2
Spermatogenic immunoglobulin superfamily
Short name:
SgIgSF
Synaptic cell adhesion molecule
Short name:
SynCAM
Tumor suppressor in lung cancer 1
Short name:
TSLC-1
Gene namesi
Name:Cadm1Imported
Synonyms:Igsf41 Publication, Necl2, Ra175, Syncam1 Publication, SynCam1Curated, Tslc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1889272 Cadm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini48 – 388ExtracellularSequence analysisAdd BLAST341
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 456CytoplasmicSequence analysisAdd BLAST47

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Male mice are infertile, due to a defect at the spermatid stage of spermatogenesis, and show oligoasthenoteratozoospermia with almost no mature motile spermatozoa in the epididymis. Heterozygous males and females and homozygous null females are fertile and have no overt developmental defects.3 Publications

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 47Sequence analysisAdd BLAST47
ChainiPRO_000029196948 – 456Cell adhesion molecule 1Sequence analysisAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi67 ↔ 127PROSITE-ProRule annotation
Glycosylationi70N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi104N-linked (GlcNAc...) asparagine3 Publications1
Glycosylationi116N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi168N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi169 ↔ 223PROSITE-ProRule annotation
Disulfide bondi270 ↔ 316PROSITE-ProRule annotation
Glycosylationi304N-linked (GlcNAc...) asparagine; atypical1 Publication1
Glycosylationi307N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi311N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei436PhosphothreonineCombined sources1
Modified residuei448PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.4 Publications
Glycosylation at Asn-70 and Asn-104 promotes adhesive binding and synapse induction.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8R5M8
PeptideAtlasiQ8R5M8
PRIDEiQ8R5M8

PTM databases

iPTMnetiQ8R5M8
PhosphoSitePlusiQ8R5M8

Expressioni

Tissue specificityi

Expressed in brain, lung, kidney, testis, heart, spleen and liver, but not expressed in skeletal muscle. In brain, enriched in the synaptic plasma membrane. Expressed dominantly in epithelial cells but not expressed in fibroblast cells (at protein level).4 Publications

Developmental stagei

Expressed in spermatogenic cells during early spermatogenesis. Expression increases in intermediate spermatogonia through to zygotene spermatocytes but becomes diminished in the steps from early pachytene spermatocytes through to round spermatids. After meiosis, expression reappears in spermatids and is present in elongating spermatids until spermiation. Not detected in Sertoli cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000032076
CleanExiMM_CADM1
ExpressionAtlasiQ8R5M8 baseline and differential
GenevisibleiQ8R5M8 MM

Interactioni

Subunit structurei

Homodimer. Interacts with CRTAM. Interacts (via C-terminus) with EPB41L3/DAL1. The interaction with EPB41L3/DAL1 may act to anchor CADM1 to the actin cytoskeleton. Interacts via its C-terminus with the PDZ domain of MPP2, MPP3 and MPP6. Interacts with FARP1.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ITGB4P161443EBI-5651941,EBI-948678From Homo sapiens.

GO - Molecular functioni

  • cell adhesion molecule binding Source: GO_Central
  • PDZ domain binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • signaling receptor binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ8R5M8, 3 interactors
MINTiQ8R5M8
STRINGi10090.ENSMUSP00000083073

Structurei

3D structure databases

ProteinModelPortaliQ8R5M8
SMRiQ8R5M8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 142Ig-like V-typeSequence analysisAdd BLAST95
Domaini147 – 241Ig-like C2-type 1Sequence analysisAdd BLAST95
Domaini246 – 332Ig-like C2-type 2Sequence analysisAdd BLAST87

Domaini

The cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC.By similarity

Sequence similaritiesi

Belongs to the nectin family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH4H Eukaryota
ENOG41116SB LUCA
GeneTreeiENSGT00900000140811
HOVERGENiHBG057086
InParanoidiQ8R5M8
KOiK06781
PhylomeDBiQ8R5M8
TreeFamiTF334317

Family and domain databases

Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR013162 CD80_C2-set
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
IPR003585 Neurexin-like
PfamiView protein in Pfam
PF08205 C2-set_2, 1 hit
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00294 4.1m, 1 hit
SM00409 IG, 3 hits
SM00408 IGc2, 3 hits
SUPFAMiSSF48726 SSF48726, 3 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 3 hits

Sequences (7)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8R5M8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASAVLPSGS QCAAAAAVAA AAAPPGLRLR LLLLLLSAAA LIPTGDGQNL
60 70 80 90 100
FTKDVTVIEG EVATISCQVN KSDDSVIQLL NPNRQTIYFR DFRPLKDSRF
110 120 130 140 150
QLLNFSSSEL KVSLTNVSIS DEGRYFCQLY TDPPQESYTT ITVLVPPRNL
160 170 180 190 200
MIDIQKDTAV EGEEIEVNCT AMASKPATTI RWFKGNKELK GKSEVEEWSD
210 220 230 240 250
MYTVTSQLML KVHKEDDGVP VICQVEHPAV TGNLQTQRYL EVQYKPQVHI
260 270 280 290 300
QMTYPLQGLT REGDAFELTC EAIGKPQPVM VTWVRVDDEM PQHAVLSGPN
310 320 330 340 350
LFINNLNKTD NGTYRCEASN IVGKAHSDYM LYVYDPPTTI PPPTTTTTTT
360 370 380 390 400
TTTTTTILTI ITDTTATTEP AVHDSRAGEE GTIGAVDHAV IGGVVAVVVF
410 420 430 440 450
AMLCLLIILG RYFARHKGTY FTHEAKGADD AADADTAIIN AEGGQNNSEE

KKEYFI
Length:456
Mass (Da):49,788
Last modified:June 26, 2007 - v2
Checksum:i3226E86C04161C7F
GO
Isoform 24 Publications (identifier: Q8R5M8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     364-374: Missing.

Show »
Length:445
Mass (Da):48,664
Checksum:iC5D5A070DAF70E55
GO
Isoform 31 Publication (identifier: Q8R5M8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-363: Missing.

Show »
Length:428
Mass (Da):46,903
Checksum:iB10DFF1A2B893573
GO
Isoform 42 Publications (identifier: Q8R5M8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-374: Missing.

Show »
Length:417
Mass (Da):45,779
Checksum:i98500180D37845C2
GO
Isoform 52 Publications (identifier: Q8R5M8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     335-336: DP → GT
     337-456: Missing.

Show »
Length:336
Mass (Da):37,155
Checksum:i9EF3D8B8BE5E8F72
GO
Isoform 61 Publication (identifier: Q8R5M8-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: Missing.
     355-365: Missing.

Show »
Length:295
Mass (Da):32,459
Checksum:iF557E3FAF8174671
GO
Isoform 71 Publication (identifier: Q8R5M8-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: Missing.
     336-352: Missing.

Show »
Length:289
Mass (Da):31,922
Checksum:iFC718DBCCEA8C452
GO

Sequence cautioni

The sequence AAC67243 differs from that shown. Reason: Frameshift at position 65.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8S → C in AAN01614 (PubMed:12202822).Curated1
Sequence conflicti8S → C in BAD30018 (Ref. 7) Curated1
Sequence conflicti266F → L in BAA87914 (PubMed:12799182).Curated1
Sequence conflicti266F → L in BAA87915 (PubMed:12799182).Curated1
Sequence conflicti315R → P in BAA87914 (PubMed:12799182).Curated1
Sequence conflicti315R → P in BAA87915 (PubMed:12799182).Curated1
Sequence conflicti330M → I in BAA87914 (PubMed:12799182).Curated1
Sequence conflicti330M → I in BAA87915 (PubMed:12799182).Curated1
Sequence conflicti356T → S in AAN01614 (PubMed:12202822).Curated1
Sequence conflicti356T → S in BAD30018 (Ref. 7) Curated1
Sequence conflicti429D → N in BAB83501 (PubMed:12799182).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0524631 – 150Missing in isoform 6 and isoform 7. 3 PublicationsAdd BLAST150
Alternative sequenceiVSP_052464335 – 336DP → GT in isoform 5. 3 Publications2
Alternative sequenceiVSP_052465336 – 374Missing in isoform 4. 2 PublicationsAdd BLAST39
Alternative sequenceiVSP_052466336 – 363Missing in isoform 3. 2 PublicationsAdd BLAST28
Alternative sequenceiVSP_052467336 – 352Missing in isoform 7. 2 PublicationsAdd BLAST17
Alternative sequenceiVSP_052468337 – 456Missing in isoform 5. 3 PublicationsAdd BLAST120
Alternative sequenceiVSP_052469355 – 365Missing in isoform 6. 2 PublicationsAdd BLAST11
Alternative sequenceiVSP_052470364 – 374Missing in isoform 2. 4 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF434663 mRNA Translation: AAL86736.1
AF539424 mRNA Translation: AAN01614.1
AB021964 mRNA Translation: BAA87914.1
AB021965 mRNA Translation: BAA87915.1
AB064265 mRNA Translation: BAB83501.2
AY351388 mRNA Translation: AAQ02381.1
AF061260 mRNA Translation: AAC67243.1 Frameshift.
AB092414 mRNA Translation: BAC66173.1
AB183399 mRNA Translation: BAD30018.1
AB183400 mRNA Translation: BAD30019.1
AB183401 mRNA Translation: BAD30020.1
AB183402 mRNA Translation: BAD30021.1
CCDSiCCDS23147.1 [Q8R5M8-4]
CCDS23148.1 [Q8R5M8-2]
CCDS23149.1 [Q8R5M8-1]
CCDS23150.1 [Q8R5M8-3]
RefSeqiNP_001020771.1, NM_001025600.1 [Q8R5M8-4]
NP_061240.3, NM_018770.3 [Q8R5M8-2]
NP_997558.2, NM_207675.2 [Q8R5M8-1]
NP_997559.1, NM_207676.2 [Q8R5M8-3]
UniGeneiMm.234832

Genome annotation databases

EnsembliENSMUST00000034581; ENSMUSP00000034581; ENSMUSG00000032076 [Q8R5M8-4]
ENSMUST00000085909; ENSMUSP00000083073; ENSMUSG00000032076 [Q8R5M8-1]
ENSMUST00000114547; ENSMUSP00000110194; ENSMUSG00000032076 [Q8R5M8-2]
ENSMUST00000114548; ENSMUSP00000110195; ENSMUSG00000032076 [Q8R5M8-3]
GeneIDi54725
KEGGimmu:54725
UCSCiuc009phn.1 mouse [Q8R5M8-5]
uc009pho.1 mouse [Q8R5M8-1]
uc009phq.1 mouse [Q8R5M8-3]
uc009phr.1 mouse [Q8R5M8-4]
uc009phs.1 mouse [Q8R5M8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCADM1_MOUSE
AccessioniPrimary (citable) accession number: Q8R5M8
Secondary accession number(s): Q6F3J3
, Q7TNL1, Q80VG4, Q8K3T6, Q8R4L1, Q9QYL5, Q9QYL6, Q9Z2H8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health