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Protein

Ubiquitin carboxyl-terminal hydrolase 33

Gene

Usp33

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei163 – 1631NucleophilePROSITE-ProRule annotation
Active sitei640 – 6401Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 33 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 33
Ubiquitin thioesterase 33
Ubiquitin-specific-processing protease 33
VHL-interacting deubiquitinating enzyme 1
Gene namesi
Name:Usp33
Synonyms:Vdu1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2159711. Usp33.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 909909Ubiquitin carboxyl-terminal hydrolase 33PRO_0000390424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei407 – 4071PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8R5K2.
PaxDbiQ8R5K2.
PRIDEiQ8R5K2.

PTM databases

PhosphoSiteiQ8R5K2.

Expressioni

Tissue specificityi

Present in E9.5 and E11.5 commissural neurons (at protein level).1 Publication

Gene expression databases

BgeeiQ8R5K2.
ExpressionAtlasiQ8R5K2. baseline and differential.
GenevisibleiQ8R5K2. MM.

Interactioni

Subunit structurei

Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2 and DIO2. Interacts with SELENBP1; in a selenium-dependent manner. Interacts with CCP110 (By similarity). Interacts with ROBO1.By similarity2 Publications

Protein-protein interaction databases

BioGridi228458. 1 interaction.
STRINGi10090.ENSMUSP00000113265.

Structurei

3D structure databases

ProteinModelPortaliQ8R5K2.
SMRiQ8R5K2. Positions 5-392, 413-683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 682529USPAdd
BLAST
Domaini684 – 77794DUSP 1PROSITE-ProRule annotationAdd
BLAST
Domaini785 – 888104DUSP 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 DUSP domains.PROSITE-ProRule annotation
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00790000122967.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiQ8R5K2.
KOiK11848.
OMAiTMEEDKS.
OrthoDBiEOG7CRTP2.
PhylomeDBiQ8R5K2.
TreeFamiTF352179.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R5K2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTFRNHCPH LDSVGEITKE DLIQKSLGAC QDCKVRGPNL WACLENRCSY
60 70 80 90 100
VGCGESQVDH STIHSQETKH YLTVNLTTLR VWCYACSKEV FLDRKLGTPP
110 120 130 140 150
SLPHVRQPQQ TQENSVQDFK IPSNPALKTP MVAVSEDLDI EVEEEDELKA
160 170 180 190 200
RGLTGLKNIG NTCYMNAALQ ALSNCPPLTQ FFLDCGGLAR TDKKPAICKS
210 220 230 240 250
YLKLMTELWH KSRPGSVVPA NLFQGIKTVN PTFRGYSQQD AQEFLRCLMD
260 270 280 290 300
LLHEELKEQV MEMEEEPQTL TSEETVEEEK SQSDVDFQSC ESCSSSEKAE
310 320 330 340 350
NESGSKGFPE DSNETTMLIQ DEDDLEMAKD WQKEKVCNKI NKANADVELD
360 370 380 390 400
KDRDTVCETV DLNSQETVKV QIHGRASESI TDVHLNDLAT SQILPSNESV
410 420 430 440 450
SPRLSASPPK LGSLWPGLSP PHKKAQSTSA KRKKQHKKYR SVISDIFDGT
460 470 480 490 500
VISSVQCLTC DRVSITLETF QDLSLPIPGK EDLAKLHSSS HPTIVKAGSC
510 520 530 540 550
GEAYAPQGWI AFFMEYVKRF VVSCVPSWFW GPVVTLQDCL AAFFARDELK
560 570 580 590 600
GDNMYSCEKC KKLRNGVKFC KVQKFPEILC IHLKRFRHEL MFSTKISTHV
610 620 630 640 650
SFPLEGLDLQ PFLAKDSPAQ IVTYDLLSVI CHHGTASSGH YIAYCRNNLN
660 670 680 690 700
NLWYEFDDQS VTEVSESTVQ NAEAYVLFYR KSSEEAQKER RRISNLLNIM
710 720 730 740 750
EPSLLQFYIS RQWLNKFKTF AEPGPISNND FLCIHGGIPP RKASYIEDLV
760 770 780 790 800
LMLPQNIWDN LYSRYGGGPA VNHLYICHTC QIELEKIEKR RKTELEIFIR
810 820 830 840 850
LNRAFQEEDS PATFYCISMQ WFREWESFVK GKDGDPPGPI DNTKIAVTKC
860 870 880 890 900
GSVMLKQGAD SGQISEETWN FLQSIYGGGP EVILRPPVVH VDPDVLQAEE

KIEVETRSL
Length:909
Mass (Da):102,728
Last modified:December 15, 2009 - v2
Checksum:iF8A2BDA9FBCD633A
GO
Isoform 2 (identifier: Q8R5K2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     519-526: Missing.

Note: No experimental confirmation available.
Show »
Length:901
Mass (Da):101,839
Checksum:i6154729A27ED06BE
GO

Sequence cautioni

The sequence AAH05506.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH89315.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti309 – 3091P → S in AAL78316 (PubMed:11739384).Curated
Sequence conflicti411 – 4111L → P in AAL78316 (PubMed:11739384).Curated
Sequence conflicti411 – 4111L → P in AAH05506 (PubMed:15489334).Curated
Sequence conflicti465 – 4651I → V in AAL78316 (PubMed:11739384).Curated
Sequence conflicti465 – 4651I → V in AAH05506 (PubMed:15489334).Curated
Sequence conflicti779 – 7791T → S in AAH89315 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei519 – 5268Missing in isoform 2. 1 PublicationVSP_038531

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF383174 mRNA. Translation: AAL78316.1.
BC005506 mRNA. Translation: AAH05506.1. Different initiation.
BC031366 mRNA. Translation: AAH31366.1.
BC089315 mRNA. Translation: AAH89315.1. Different initiation.
AK143844 mRNA. Translation: BAE25565.1.
CCDSiCCDS17918.1. [Q8R5K2-1]
CCDS57261.1. [Q8R5K2-2]
RefSeqiNP_001239415.1. NM_001252486.1. [Q8R5K2-2]
NP_573510.2. NM_133247.3. [Q8R5K2-1]
UniGeneiMm.258320.

Genome annotation databases

EnsembliENSMUST00000026507; ENSMUSP00000026507; ENSMUSG00000025437. [Q8R5K2-2]
ENSMUST00000117492; ENSMUSP00000113265; ENSMUSG00000025437. [Q8R5K2-1]
GeneIDi170822.
KEGGimmu:170822.
UCSCiuc008rti.2. mouse. [Q8R5K2-1]
uc008rtj.2. mouse. [Q8R5K2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF383174 mRNA. Translation: AAL78316.1.
BC005506 mRNA. Translation: AAH05506.1. Different initiation.
BC031366 mRNA. Translation: AAH31366.1.
BC089315 mRNA. Translation: AAH89315.1. Different initiation.
AK143844 mRNA. Translation: BAE25565.1.
CCDSiCCDS17918.1. [Q8R5K2-1]
CCDS57261.1. [Q8R5K2-2]
RefSeqiNP_001239415.1. NM_001252486.1. [Q8R5K2-2]
NP_573510.2. NM_133247.3. [Q8R5K2-1]
UniGeneiMm.258320.

3D structure databases

ProteinModelPortaliQ8R5K2.
SMRiQ8R5K2. Positions 5-392, 413-683.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228458. 1 interaction.
STRINGi10090.ENSMUSP00000113265.

PTM databases

PhosphoSiteiQ8R5K2.

Proteomic databases

MaxQBiQ8R5K2.
PaxDbiQ8R5K2.
PRIDEiQ8R5K2.

Protocols and materials databases

DNASUi170822.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026507; ENSMUSP00000026507; ENSMUSG00000025437. [Q8R5K2-2]
ENSMUST00000117492; ENSMUSP00000113265; ENSMUSG00000025437. [Q8R5K2-1]
GeneIDi170822.
KEGGimmu:170822.
UCSCiuc008rti.2. mouse. [Q8R5K2-1]
uc008rtj.2. mouse. [Q8R5K2-2]

Organism-specific databases

CTDi23032.
MGIiMGI:2159711. Usp33.

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00790000122967.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
InParanoidiQ8R5K2.
KOiK11848.
OMAiTMEEDKS.
OrthoDBiEOG7CRTP2.
PhylomeDBiQ8R5K2.
TreeFamiTF352179.

Miscellaneous databases

NextBioi370477.
PROiQ8R5K2.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R5K2.
ExpressionAtlasiQ8R5K2. baseline and differential.
GenevisibleiQ8R5K2. MM.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein."
    Li Z., Na X., Wang D., Schoen S.R., Messing E.M., Wu G.
    J. Biol. Chem. 277:4656-4662(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 225-909 (ISOFORM 1).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain, Eye and Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-880 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Spleen.
  4. "Midline crossing and Slit responsiveness of commissural axons require USP33."
    Yuasa-Kawada J., Kinoshita-Kawada M., Wu G., Rao Y., Wu J.Y.
    Nat. Neurosci. 12:1087-1089(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ROBO1, TISSUE SPECIFICITY.
  5. "Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration."
    Yuasa-Kawada J., Kinoshita-Kawada M., Rao Y., Wu J.Y.
    Proc. Natl. Acad. Sci. U.S.A. 106:14530-14535(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ROBO1.

Entry informationi

Entry nameiUBP33_MOUSE
AccessioniPrimary (citable) accession number: Q8R5K2
Secondary accession number(s): Q3UP32
, Q5FWK0, Q8K0I3, Q99K22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 15, 2009
Last modified: June 24, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.