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Q8R5J9 (PRAF3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name=ARL-6-interacting protein 5
Short name=Aip-5
Addicsin
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
Prenylated Rab acceptor protein 2
Protein JWa
Gene names
Name:Arl6ip5
Synonyms:Aip5, Jwa, Pra2, Praf3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic. Ref.1

Subunit structure

Binds to prenylated RAB1A and RAB3A, and to the neuronal glutamate transporter SLC1A1/EAAC1. Does not bind to VAMP2 By similarity. Forms multimers. Binds to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily. Ref.1 Ref.6

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm. Cytoplasmcytoskeleton. Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules. Ref.2

Tissue specificity

Ubiquitous. Ref.1 Ref.2

Induction

By methyl-beta-cyclodextrin. Up-regulated upon chronic morphine injection, in amygdala only, other brain regions remain unaffected. Ref.2

Miscellaneous

Induction by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence.

Sequence similarities

Belongs to the PRA1 family.

Caution

Was originally (Ref.8) reported to be induced by retinoic acid.

Sequence caution

The sequence AAD33050.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

The sequence AAD33050.1 differs from that shown. Reason: Frameshift at positions 94 and 108.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188PRA1 family protein 3
PRO_0000220884

Regions

Topological domain1 – 3939Cytoplasmic By similarity
Transmembrane40 – 6021Helical; Potential
Transmembrane64 – 8421Helical; Potential
Topological domain85 – 928Cytoplasmic By similarity
Transmembrane93 – 11321Helical; Potential
Transmembrane115 – 13521Helical; Potential
Topological domain136 – 18853Cytoplasmic By similarity
Region136 – 18853Targeting to endoplasmic reticulum membrane By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Experimental info

Sequence conflict111W → R in BAC40209. Ref.4
Sequence conflict141F → S in BAC40209. Ref.4
Sequence conflict191Missing Ref.6
Sequence conflict861Missing Ref.6
Sequence conflict1031M → T in BAB25717. Ref.4
Sequence conflict1171G → R Ref.6
Sequence conflict1401R → K in BAB25717. Ref.4
Sequence conflict1631G → V in AAL77876. Ref.1
Sequence conflict1781K → E in BAC40209. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8R5J9 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 5A679FC5071319F0

FASTA18821,558
        10         20         30         40         50         60 
MDVNLAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV AAMMISVVGF 

        70         80         90        100        110        120 
LSPFNMILGG VIVVLVFMGF VWAAHNKDIL RRMKKQYPTA FVMVVMLASY FLISMFGGVM 

       130        140        150        160        170        180 
VFVFGITLPL LLMFIHASLR LRNLKNKLEN KMEGIGLKKT PMGIILDALE QQEDNINKFA 


DYISKARE 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning, gene structure, expression profile and functional characterization of the mouse glutamate transporter (EAAT3) interacting protein GTRAP3-18."
Butchbach M.E.R., Lai L., Lin C.-L.G.
Gene 292:81-90(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SLC1A1, TISSUE SPECIFICITY.
Strain: C57BL/6J.
Tissue: Brain.
[2]"Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala."
Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M.
NeuroReport 13:2079-2084(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
Tissue: Amygdala.
[3]"JWa and its biological evolution."
Xia W., Cao H.X., Zhou J.W.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Cerebellum, Head, Small intestine and Thymus.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[6]"A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit."
Ingley E., Williams J.H., Walker C.E., Tsai S., Colley S., Sayer M.S., Tilbrook P.A., Sarna M., Beaumont J.G., Klinken S.P.
FEBS Lett. 459:69-74(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-118, INTERACTION WITH ARL6.
[7]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 10-27 AND 159-178, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[8]"Methyl-beta-cyclodextrin but not retinoic acid reduces EAAT3-mediated glutamate uptake and increases GTRAP3-18 expression."
Butchbach M.E.R., Guo H., Lin C.-L.G.
J. Neurochem. 84:891-894(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF421860 mRNA. Translation: AAL77876.1.
D87211 mRNA. Translation: BAC24103.1.
AF265214 mRNA. Translation: AAL74056.1.
AK005259 mRNA. Translation: BAB23912.1.
AK008519 mRNA. Translation: BAB25717.1.
AK088205 mRNA. Translation: BAC40209.1.
AK076519 mRNA. Translation: BAC36376.1.
BC003897 mRNA. Translation: AAH03897.1.
AF133912 mRNA. Translation: AAD33050.1. Sequence problems.
RefSeqNP_075368.1. NM_022992.2.
UniGeneMm.291014.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8R5J9. 3 interactions.
MINTMINT-219147.

PTM databases

PhosphoSiteQ8R5J9.

Proteomic databases

PaxDbQ8R5J9.
PRIDEQ8R5J9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044681; ENSMUSP00000041503; ENSMUSG00000035199.
GeneID65106.
KEGGmmu:65106.
UCSCuc009dar.1. mouse.

Organism-specific databases

CTD10550.
MGIMGI:1929501. Arl6ip5.

Phylogenomic databases

eggNOGNOG272388.
GeneTreeENSGT00390000008631.
HOGENOMHOG000231363.
HOVERGENHBG053661.
InParanoidQ8R5J9.
OMAYPTAFVM.
OrthoDBEOG7VDXQH.
PhylomeDBQ8R5J9.
TreeFamTF105479.

Gene expression databases

BgeeQ8R5J9.
GenevestigatorQ8R5J9.

Family and domain databases

InterProIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARL6IP5. mouse.
NextBio320293.
PROQ8R5J9.
SOURCESearch...

Entry information

Entry namePRAF3_MOUSE
AccessionPrimary (citable) accession number: Q8R5J9
Secondary accession number(s): Q8C2Q1 expand/collapse secondary AC list , Q9D838, Q9DB37, Q9WUG9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot