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Protein

PRA1 family protein 3

Gene

Arl6ip5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates intracellular concentrations of taurine and glutamate (By similarity). Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner (PubMed:12119102, PubMed:18684713). May be involved in membrane traffic (By similarity).By similarity2 Publications

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name:
ARL-6-interacting protein 5
Short name:
Aip-5
Addicsin
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
Prenylated Rab acceptor protein 2
Protein JWa
Gene namesi
Name:Arl6ip5
Synonyms:Aip5, Jwa, Pra2, Praf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1929501. Arl6ip5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 39CytoplasmicBy similarityAdd BLAST39
Transmembranei40 – 60HelicalSequence analysisAdd BLAST21
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Topological domaini85 – 92CytoplasmicBy similarity8
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Topological domaini136 – 188CytoplasmicBy similarityAdd BLAST53

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110Y → A: Significant decrease in interaction with ARL6IP1 and no influence on SLC1A1/EAAC1-mediated glutamate transport; when associated with A-112. 1 Publication1
Mutagenesisi112L → A: Significant decrease in interaction with ARL6IP1 and no influence on SLC1A1/EAAC1-mediated glutamate transport; when associated with A-110. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208841 – 188PRA1 family protein 3Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8R5J9.
MaxQBiQ8R5J9.
PaxDbiQ8R5J9.
PeptideAtlasiQ8R5J9.
PRIDEiQ8R5J9.

PTM databases

iPTMnetiQ8R5J9.
PhosphoSitePlusiQ8R5J9.

Expressioni

Tissue specificityi

Expressed in the cerebral cortex, cerebellum, hippocampus, olfactory bulbs, medulla oblongate and limbic system (at protein level) (PubMed:18684713). Ubiquitous.3 Publications

Inductioni

By methyl-beta-cyclodextrin. Up-regulated upon chronic morphine injection, in amygdala only, other brain regions remain unaffected. Induction by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence (PubMed:12438930). Was originally reported to be induced by retinoic acid (PubMed:12562531).1 Publication1 Publication

Gene expression databases

BgeeiENSMUSG00000035199.
GenevisibleiQ8R5J9. MM.

Interactioni

Subunit structurei

Homodimer. Heterodimer with ARL6IP1 (PubMed:18684713). Forms multimers. Interacts with prenylated RAB1A and RAB3A. Does not interact with VAMP1, VAMP2 or VAMP3 (By similarity). Interacts with ARL6 (PubMed:10508919). Interacts with SLC1A1/EAAC1 (PubMed:18684713, PubMed:12119102).By similarity3 Publications

Protein-protein interaction databases

IntActiQ8R5J9. 3 interactors.
MINTiMINT-219147.
STRINGi10090.ENSMUSP00000041503.

Structurei

3D structure databases

ProteinModelPortaliQ8R5J9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 117Required for homodimer formation and heterodimer formation with ARL6IP11 PublicationAdd BLAST15
Regioni136 – 188Targeting to endoplasmic reticulum membraneBy similarityAdd BLAST53

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4050. Eukaryota.
ENOG4111MUC. LUCA.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiQ8R5J9.
KOiK20393.
OMAiTNYLMVA.
OrthoDBiEOG091G0QS5.
PhylomeDBiQ8R5J9.
TreeFamiTF105479.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R5J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVNLAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV
60 70 80 90 100
AAMMISVVGF LSPFNMILGG VIVVLVFMGF VWAAHNKDIL RRMKKQYPTA
110 120 130 140 150
FVMVVMLASY FLISMFGGVM VFVFGITLPL LLMFIHASLR LRNLKNKLEN
160 170 180
KMEGIGLKKT PMGIILDALE QQEDNINKFA DYISKARE
Length:188
Mass (Da):21,558
Last modified:January 4, 2005 - v2
Checksum:i5A679FC5071319F0
GO

Sequence cautioni

The sequence AAD33050 differs from that shown. Reason: Frameshift at positions 94 and 108.Curated
The sequence AAD33050 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11W → R in BAC40209 (PubMed:16141072).Curated1
Sequence conflicti14F → S in BAC40209 (PubMed:16141072).Curated1
Sequence conflicti19Missing (PubMed:10508919).Curated1
Sequence conflicti86Missing (PubMed:10508919).Curated1
Sequence conflicti103M → T in BAB25717 (PubMed:16141072).Curated1
Sequence conflicti117G → R (PubMed:10508919).Curated1
Sequence conflicti140R → K in BAB25717 (PubMed:16141072).Curated1
Sequence conflicti163G → V in AAL77876 (PubMed:12119102).Curated1
Sequence conflicti178K → E in BAC40209 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF421860 mRNA. Translation: AAL77876.1.
D87211 mRNA. Translation: BAC24103.1.
AF265214 mRNA. Translation: AAL74056.1.
AK005259 mRNA. Translation: BAB23912.1.
AK008519 mRNA. Translation: BAB25717.1.
AK088205 mRNA. Translation: BAC40209.1.
AK076519 mRNA. Translation: BAC36376.1.
BC003897 mRNA. Translation: AAH03897.1.
AF133912 mRNA. Translation: AAD33050.1. Sequence problems.
CCDSiCCDS20384.1.
RefSeqiNP_075368.1. NM_022992.2.
UniGeneiMm.291014.

Genome annotation databases

EnsembliENSMUST00000044681; ENSMUSP00000041503; ENSMUSG00000035199.
GeneIDi65106.
KEGGimmu:65106.
UCSCiuc009dar.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF421860 mRNA. Translation: AAL77876.1.
D87211 mRNA. Translation: BAC24103.1.
AF265214 mRNA. Translation: AAL74056.1.
AK005259 mRNA. Translation: BAB23912.1.
AK008519 mRNA. Translation: BAB25717.1.
AK088205 mRNA. Translation: BAC40209.1.
AK076519 mRNA. Translation: BAC36376.1.
BC003897 mRNA. Translation: AAH03897.1.
AF133912 mRNA. Translation: AAD33050.1. Sequence problems.
CCDSiCCDS20384.1.
RefSeqiNP_075368.1. NM_022992.2.
UniGeneiMm.291014.

3D structure databases

ProteinModelPortaliQ8R5J9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R5J9. 3 interactors.
MINTiMINT-219147.
STRINGi10090.ENSMUSP00000041503.

PTM databases

iPTMnetiQ8R5J9.
PhosphoSitePlusiQ8R5J9.

Proteomic databases

EPDiQ8R5J9.
MaxQBiQ8R5J9.
PaxDbiQ8R5J9.
PeptideAtlasiQ8R5J9.
PRIDEiQ8R5J9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044681; ENSMUSP00000041503; ENSMUSG00000035199.
GeneIDi65106.
KEGGimmu:65106.
UCSCiuc009dar.1. mouse.

Organism-specific databases

CTDi10550.
MGIiMGI:1929501. Arl6ip5.

Phylogenomic databases

eggNOGiKOG4050. Eukaryota.
ENOG4111MUC. LUCA.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiQ8R5J9.
KOiK20393.
OMAiTNYLMVA.
OrthoDBiEOG091G0QS5.
PhylomeDBiQ8R5J9.
TreeFamiTF105479.

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

ChiTaRSiArl6ip5. mouse.
PROiQ8R5J9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035199.
GenevisibleiQ8R5J9. MM.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRAF3_MOUSE
AccessioniPrimary (citable) accession number: Q8R5J9
Secondary accession number(s): Q8C2Q1
, Q9D838, Q9DB37, Q9WUG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.