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Q8R5J9

- PRAF3_MOUSE

UniProt

Q8R5J9 - PRAF3_MOUSE

Protein

PRA1 family protein 3

Gene

Arl6ip5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic.1 Publication

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. L-glutamate transport Source: MGI
    2. negative regulation of transport Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PRA1 family protein 3
    Alternative name(s):
    ADP-ribosylation factor-like protein 6-interacting protein 5
    Short name:
    ARL-6-interacting protein 5
    Short name:
    Aip-5
    Addicsin
    GTRAP3-18
    Glutamate transporter EAAC1-interacting protein
    Prenylated Rab acceptor protein 2
    Protein JWa
    Gene namesi
    Name:Arl6ip5
    Synonyms:Aip5, Jwa, Pra2, Praf3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1929501. Arl6ip5.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication
    Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules.

    GO - Cellular componenti

    1. cytoskeleton Source: UniProtKB-SubCell
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188PRA1 family protein 3PRO_0000220884Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8R5J9.
    PaxDbiQ8R5J9.
    PRIDEiQ8R5J9.

    PTM databases

    PhosphoSiteiQ8R5J9.

    Expressioni

    Tissue specificityi

    Ubiquitous.2 Publications

    Inductioni

    By methyl-beta-cyclodextrin. Up-regulated upon chronic morphine injection, in amygdala only, other brain regions remain unaffected.1 Publication

    Gene expression databases

    BgeeiQ8R5J9.
    GenevestigatoriQ8R5J9.

    Interactioni

    Subunit structurei

    Binds to prenylated RAB1A and RAB3A, and to the neuronal glutamate transporter SLC1A1/EAAC1. Does not bind to VAMP2 By similarity. Forms multimers. Binds to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily.By similarity

    Protein-protein interaction databases

    IntActiQ8R5J9. 3 interactions.
    MINTiMINT-219147.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3939CytoplasmicBy similarityAdd
    BLAST
    Topological domaini85 – 928CytoplasmicBy similarity
    Topological domaini136 – 18853CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei40 – 6021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei93 – 11321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei115 – 13521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni136 – 18853Targeting to endoplasmic reticulum membraneBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PRA1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG272388.
    GeneTreeiENSGT00390000008631.
    HOGENOMiHOG000231363.
    HOVERGENiHBG053661.
    InParanoidiQ8R5J9.
    OMAiKMNKIQD.
    OrthoDBiEOG7VDXQH.
    PhylomeDBiQ8R5J9.
    TreeFamiTF105479.

    Family and domain databases

    InterProiIPR004895. Prenylated_rab_accept_PRA1.
    [Graphical view]
    PfamiPF03208. PRA1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R5J9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVNLAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV    50
    AAMMISVVGF LSPFNMILGG VIVVLVFMGF VWAAHNKDIL RRMKKQYPTA 100
    FVMVVMLASY FLISMFGGVM VFVFGITLPL LLMFIHASLR LRNLKNKLEN 150
    KMEGIGLKKT PMGIILDALE QQEDNINKFA DYISKARE 188
    Length:188
    Mass (Da):21,558
    Last modified:January 4, 2005 - v2
    Checksum:i5A679FC5071319F0
    GO

    Sequence cautioni

    The sequence AAD33050.1 differs from that shown. Reason: Frameshift at positions 94 and 108.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111W → R in BAC40209. (PubMed:16141072)Curated
    Sequence conflicti14 – 141F → S in BAC40209. (PubMed:16141072)Curated
    Sequence conflicti19 – 191Missing(PubMed:10508919)Curated
    Sequence conflicti86 – 861Missing(PubMed:10508919)Curated
    Sequence conflicti103 – 1031M → T in BAB25717. (PubMed:16141072)Curated
    Sequence conflicti117 – 1171G → R(PubMed:10508919)Curated
    Sequence conflicti140 – 1401R → K in BAB25717. (PubMed:16141072)Curated
    Sequence conflicti163 – 1631G → V in AAL77876. (PubMed:12119102)Curated
    Sequence conflicti178 – 1781K → E in BAC40209. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF421860 mRNA. Translation: AAL77876.1.
    D87211 mRNA. Translation: BAC24103.1.
    AF265214 mRNA. Translation: AAL74056.1.
    AK005259 mRNA. Translation: BAB23912.1.
    AK008519 mRNA. Translation: BAB25717.1.
    AK088205 mRNA. Translation: BAC40209.1.
    AK076519 mRNA. Translation: BAC36376.1.
    BC003897 mRNA. Translation: AAH03897.1.
    AF133912 mRNA. Translation: AAD33050.1. Sequence problems.
    CCDSiCCDS20384.1.
    RefSeqiNP_075368.1. NM_022992.2.
    UniGeneiMm.291014.

    Genome annotation databases

    EnsembliENSMUST00000044681; ENSMUSP00000041503; ENSMUSG00000035199.
    GeneIDi65106.
    KEGGimmu:65106.
    UCSCiuc009dar.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF421860 mRNA. Translation: AAL77876.1 .
    D87211 mRNA. Translation: BAC24103.1 .
    AF265214 mRNA. Translation: AAL74056.1 .
    AK005259 mRNA. Translation: BAB23912.1 .
    AK008519 mRNA. Translation: BAB25717.1 .
    AK088205 mRNA. Translation: BAC40209.1 .
    AK076519 mRNA. Translation: BAC36376.1 .
    BC003897 mRNA. Translation: AAH03897.1 .
    AF133912 mRNA. Translation: AAD33050.1 . Sequence problems.
    CCDSi CCDS20384.1.
    RefSeqi NP_075368.1. NM_022992.2.
    UniGenei Mm.291014.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8R5J9. 3 interactions.
    MINTi MINT-219147.

    PTM databases

    PhosphoSitei Q8R5J9.

    Proteomic databases

    MaxQBi Q8R5J9.
    PaxDbi Q8R5J9.
    PRIDEi Q8R5J9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000044681 ; ENSMUSP00000041503 ; ENSMUSG00000035199 .
    GeneIDi 65106.
    KEGGi mmu:65106.
    UCSCi uc009dar.1. mouse.

    Organism-specific databases

    CTDi 10550.
    MGIi MGI:1929501. Arl6ip5.

    Phylogenomic databases

    eggNOGi NOG272388.
    GeneTreei ENSGT00390000008631.
    HOGENOMi HOG000231363.
    HOVERGENi HBG053661.
    InParanoidi Q8R5J9.
    OMAi KMNKIQD.
    OrthoDBi EOG7VDXQH.
    PhylomeDBi Q8R5J9.
    TreeFami TF105479.

    Miscellaneous databases

    ChiTaRSi ARL6IP5. mouse.
    NextBioi 320293.
    PROi Q8R5J9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8R5J9.
    Genevestigatori Q8R5J9.

    Family and domain databases

    InterProi IPR004895. Prenylated_rab_accept_PRA1.
    [Graphical view ]
    Pfami PF03208. PRA1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, gene structure, expression profile and functional characterization of the mouse glutamate transporter (EAAT3) interacting protein GTRAP3-18."
      Butchbach M.E.R., Lai L., Lin C.-L.G.
      Gene 292:81-90(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SLC1A1, TISSUE SPECIFICITY.
      Strain: C57BL/6J.
      Tissue: Brain.
    2. "Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala."
      Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M.
      NeuroReport 13:2079-2084(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
      Tissue: Amygdala.
    3. "JWa and its biological evolution."
      Xia W., Cao H.X., Zhou J.W.
      Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Cerebellum, Head, Small intestine and Thymus.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    6. "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit."
      Ingley E., Williams J.H., Walker C.E., Tsai S., Colley S., Sayer M.S., Tilbrook P.A., Sarna M., Beaumont J.G., Klinken S.P.
      FEBS Lett. 459:69-74(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-118, INTERACTION WITH ARL6.
    7. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 10-27 AND 159-178, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    8. "Methyl-beta-cyclodextrin but not retinoic acid reduces EAAT3-mediated glutamate uptake and increases GTRAP3-18 expression."
      Butchbach M.E.R., Guo H., Lin C.-L.G.
      J. Neurochem. 84:891-894(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiPRAF3_MOUSE
    AccessioniPrimary (citable) accession number: Q8R5J9
    Secondary accession number(s): Q8C2Q1
    , Q9D838, Q9DB37, Q9WUG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Induction by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence.

    Caution

    Was originally reported to be induced by retinoic acid.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3