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Q8R5J9

- PRAF3_MOUSE

UniProt

Q8R5J9 - PRAF3_MOUSE

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Protein

PRA1 family protein 3

Gene

Arl6ip5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate. May be involved in membrane traffic.1 Publication

GO - Biological processi

  1. intrinsic apoptotic signaling pathway in response to oxidative stress Source: Ensembl
  2. L-glutamate transport Source: MGI
  3. negative regulation of mitochondrial membrane potential Source: Ensembl
  4. negative regulation of transport Source: Ensembl
  5. positive regulation of apoptotic process Source: Ensembl
  6. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  7. positive regulation of stress-activated MAPK cascade Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name:
ARL-6-interacting protein 5
Short name:
Aip-5
Addicsin
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
Prenylated Rab acceptor protein 2
Protein JWa
Gene namesi
Name:Arl6ip5
Synonyms:Aip5, Jwa, Pra2, Praf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1929501. Arl6ip5.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasm 1 Publication. Cytoplasmcytoskeleton 1 Publication
Note: Also exists as a soluble form in the cytoplasm. Associated with microtubules.

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-KW
  2. endoplasmic reticulum Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: Ensembl
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188PRA1 family protein 3PRO_0000220884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8R5J9.
PaxDbiQ8R5J9.
PRIDEiQ8R5J9.

PTM databases

PhosphoSiteiQ8R5J9.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Inductioni

By methyl-beta-cyclodextrin. Up-regulated upon chronic morphine injection, in amygdala only, other brain regions remain unaffected.1 Publication

Gene expression databases

BgeeiQ8R5J9.
GenevestigatoriQ8R5J9.

Interactioni

Subunit structurei

Binds to prenylated RAB1A and RAB3A, and to the neuronal glutamate transporter SLC1A1/EAAC1. Does not bind to VAMP2 By similarity. Forms multimers. Binds to the ADP-ribosylation like factor ARL6, a member of the Ras superfamily.By similarity

Protein-protein interaction databases

IntActiQ8R5J9. 3 interactions.
MINTiMINT-219147.

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939CytoplasmicBy similarityAdd
BLAST
Topological domaini85 – 928CytoplasmicBy similarity
Topological domaini136 – 18853CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei40 – 6021HelicalSequence AnalysisAdd
BLAST
Transmembranei64 – 8421HelicalSequence AnalysisAdd
BLAST
Transmembranei93 – 11321HelicalSequence AnalysisAdd
BLAST
Transmembranei115 – 13521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 18853Targeting to endoplasmic reticulum membraneBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG272388.
GeneTreeiENSGT00390000008631.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiQ8R5J9.
OMAiKMNKIQD.
OrthoDBiEOG7VDXQH.
PhylomeDBiQ8R5J9.
TreeFamiTF105479.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R5J9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVNLAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV
60 70 80 90 100
AAMMISVVGF LSPFNMILGG VIVVLVFMGF VWAAHNKDIL RRMKKQYPTA
110 120 130 140 150
FVMVVMLASY FLISMFGGVM VFVFGITLPL LLMFIHASLR LRNLKNKLEN
160 170 180
KMEGIGLKKT PMGIILDALE QQEDNINKFA DYISKARE
Length:188
Mass (Da):21,558
Last modified:January 4, 2005 - v2
Checksum:i5A679FC5071319F0
GO

Sequence cautioni

The sequence AAD33050.1 differs from that shown. Reason: Frameshift at positions 94 and 108.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111W → R in BAC40209. (PubMed:16141072)Curated
Sequence conflicti14 – 141F → S in BAC40209. (PubMed:16141072)Curated
Sequence conflicti19 – 191Missing(PubMed:10508919)Curated
Sequence conflicti86 – 861Missing(PubMed:10508919)Curated
Sequence conflicti103 – 1031M → T in BAB25717. (PubMed:16141072)Curated
Sequence conflicti117 – 1171G → R(PubMed:10508919)Curated
Sequence conflicti140 – 1401R → K in BAB25717. (PubMed:16141072)Curated
Sequence conflicti163 – 1631G → V in AAL77876. (PubMed:12119102)Curated
Sequence conflicti178 – 1781K → E in BAC40209. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF421860 mRNA. Translation: AAL77876.1.
D87211 mRNA. Translation: BAC24103.1.
AF265214 mRNA. Translation: AAL74056.1.
AK005259 mRNA. Translation: BAB23912.1.
AK008519 mRNA. Translation: BAB25717.1.
AK088205 mRNA. Translation: BAC40209.1.
AK076519 mRNA. Translation: BAC36376.1.
BC003897 mRNA. Translation: AAH03897.1.
AF133912 mRNA. Translation: AAD33050.1. Sequence problems.
CCDSiCCDS20384.1.
RefSeqiNP_075368.1. NM_022992.2.
UniGeneiMm.291014.

Genome annotation databases

EnsembliENSMUST00000044681; ENSMUSP00000041503; ENSMUSG00000035199.
GeneIDi65106.
KEGGimmu:65106.
UCSCiuc009dar.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF421860 mRNA. Translation: AAL77876.1 .
D87211 mRNA. Translation: BAC24103.1 .
AF265214 mRNA. Translation: AAL74056.1 .
AK005259 mRNA. Translation: BAB23912.1 .
AK008519 mRNA. Translation: BAB25717.1 .
AK088205 mRNA. Translation: BAC40209.1 .
AK076519 mRNA. Translation: BAC36376.1 .
BC003897 mRNA. Translation: AAH03897.1 .
AF133912 mRNA. Translation: AAD33050.1 . Sequence problems.
CCDSi CCDS20384.1.
RefSeqi NP_075368.1. NM_022992.2.
UniGenei Mm.291014.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8R5J9. 3 interactions.
MINTi MINT-219147.

PTM databases

PhosphoSitei Q8R5J9.

Proteomic databases

MaxQBi Q8R5J9.
PaxDbi Q8R5J9.
PRIDEi Q8R5J9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000044681 ; ENSMUSP00000041503 ; ENSMUSG00000035199 .
GeneIDi 65106.
KEGGi mmu:65106.
UCSCi uc009dar.1. mouse.

Organism-specific databases

CTDi 10550.
MGIi MGI:1929501. Arl6ip5.

Phylogenomic databases

eggNOGi NOG272388.
GeneTreei ENSGT00390000008631.
HOGENOMi HOG000231363.
HOVERGENi HBG053661.
InParanoidi Q8R5J9.
OMAi KMNKIQD.
OrthoDBi EOG7VDXQH.
PhylomeDBi Q8R5J9.
TreeFami TF105479.

Miscellaneous databases

ChiTaRSi ARL6IP5. mouse.
NextBioi 320293.
PROi Q8R5J9.
SOURCEi Search...

Gene expression databases

Bgeei Q8R5J9.
Genevestigatori Q8R5J9.

Family and domain databases

InterProi IPR004895. Prenylated_rab_accept_PRA1.
[Graphical view ]
Pfami PF03208. PRA1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, gene structure, expression profile and functional characterization of the mouse glutamate transporter (EAAT3) interacting protein GTRAP3-18."
    Butchbach M.E.R., Lai L., Lin C.-L.G.
    Gene 292:81-90(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SLC1A1, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Identification of addicsin/GTRAP3-18 as a chronic morphine-augumented gene in amygdala."
    Ikemoto M.J., Inoue K., Akiduki S., Osugi T., Imamura T., Ishida N., Ohtomi M.
    NeuroReport 13:2079-2084(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
    Tissue: Amygdala.
  3. "JWa and its biological evolution."
    Xia W., Cao H.X., Zhou J.W.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Cerebellum, Head, Small intestine and Thymus.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  6. "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit."
    Ingley E., Williams J.H., Walker C.E., Tsai S., Colley S., Sayer M.S., Tilbrook P.A., Sarna M., Beaumont J.G., Klinken S.P.
    FEBS Lett. 459:69-74(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-118, INTERACTION WITH ARL6.
  7. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 10-27 AND 159-178, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  8. "Methyl-beta-cyclodextrin but not retinoic acid reduces EAAT3-mediated glutamate uptake and increases GTRAP3-18 expression."
    Butchbach M.E.R., Guo H., Lin C.-L.G.
    J. Neurochem. 84:891-894(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiPRAF3_MOUSE
AccessioniPrimary (citable) accession number: Q8R5J9
Secondary accession number(s): Q8C2Q1
, Q9D838, Q9DB37, Q9WUG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Induction by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence.

Caution

Was originally reported to be induced by retinoic acid.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3