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Protein

Myocardin

Gene

Myocd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes (By similarity). Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell growth involved in cardiac muscle cell development Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • cellular response to hypoxia Source: RGD
  • hepatic stellate cell activation Source: RGD
  • positive regulation of cardiac muscle cell differentiation Source: InterPro
  • positive regulation of cardiac vascular smooth muscle cell differentiation Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation Source: BHF-UCL
  • regulation of histone acetylation Source: UniProtKB
  • regulation of smooth muscle cell differentiation Source: RGD
  • smooth muscle cell differentiation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Myocardin
Gene namesi
Name:Myocd
Synonyms:Mycd
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi631347. Myocd.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi534I → T: Abolishes homodimerization. 1 Publication1
Mutagenesisi537L → T: Abolishes homodimerization. 1 Publication1
Mutagenesisi541L → T: Abolishes homodimerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001266331 – 938MyocardinAdd BLAST938

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei457Phosphoserine; by GSK3-betaBy similarity1
Modified residuei461Phosphoserine; by GSK3-betaBy similarity1
Modified residuei465Phosphoserine; by GSK3-betaBy similarity1
Modified residuei469Phosphoserine; by GSK3-betaBy similarity1
Modified residuei627Phosphoserine; by GSK3-betaBy similarity1
Modified residuei631Phosphoserine; by GSK3-betaBy similarity1
Modified residuei635Phosphoserine; by GSK3-betaBy similarity1
Modified residuei639Phosphoserine; by GSK3-betaBy similarity1
Modified residuei815Phosphoserine; by MAPK1 AND MAPK3By similarity1
Modified residuei862Phosphoserine; by MAPK1 AND MAPK3By similarity1
Modified residuei869Phosphoserine; by MAPK1 AND MAPK3By similarity1
Modified residuei896Phosphothreonine; by MAPK1 AND MAPK3By similarity1

Post-translational modificationi

Phosphorylation regulates negatively transcriptional activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8R5I7.
PRIDEiQ8R5I7.

Expressioni

Tissue specificityi

High expression in heart, aorta media and bladder.1 Publication

Gene expression databases

BgeeiENSRNOG00000003669.
ExpressionAtlasiQ8R5I7. baseline and differential.
GenevisibleiQ8R5I7. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with MLLT7/FOXO4. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000054372.

Structurei

3D structure databases

ProteinModelPortaliQ8R5I7.
SMRiQ8R5I7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 43RPEL 1Add BLAST26
Repeati62 – 87RPEL 2Add BLAST26
Repeati106 – 131RPEL 3Add BLAST26
Domaini383 – 417SAPPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 205HDAC5-bindingBy similarityAdd BLAST53

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili522 – 566Sequence analysisAdd BLAST45

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 27MEF2C-bindingBy similarityAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi291 – 323Gln-richAdd BLAST33

Domaini

The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).By similarity

Sequence similaritiesi

Contains 3 RPEL repeats.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IE2C. Eukaryota.
ENOG410XNR1. LUCA.
GeneTreeiENSGT00530000063195.
HOGENOMiHOG000038001.
HOVERGENiHBG036493.
InParanoidiQ8R5I7.
PhylomeDBiQ8R5I7.
TreeFamiTF326024.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR028721. MYOCD.
IPR004018. RPEL_repeat.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR22793:SF11. PTHR22793:SF11. 2 hits.
PfamiPF02755. RPEL. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R5I7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLGSEHSL LIRRKFRSVL QLRLQQRRTQ EQLANQGLIP PLKSPTEFHD
60 70 80 90 100
PRKKLDSAKT EDSLRRKVRN RSDRASLVNM HILQASTAER SIPTAQMKLK
110 120 130 140 150
RARLADDLNE KIALRPGPLE LVEKNILPMD SSVKEAIKGT EVSLSKAADA
160 170 180 190 200
FAFEDDSSRD GLSPDQARSE DPQGSGGSTP DIKSTEAPLA GPLDTIQDLT
210 220 230 240 250
PGSESDKNDT ASQLSNQSDS GKQVLGPLST PIPVHTAVKS KSLGDSKNRH
260 270 280 290 300
KKPKDPKPKV KKLKYHQYIP PDQKAEKSPP PMDSAYARLL QQQQLFLQLQ
310 320 330 340 350
ILSQQQQQQQ QQQQQQQQQQ QQQRFSYPGM HQAHLKEPNE QMTRNPNSSS
360 370 380 390 400
TPLNNTPLSP VKNSLSGQTG VSSLKPGPLP PNLDDLKVSE LRQQLRIRGL
410 420 430 440 450
PVSGTKTALV DRLRPFQDCA GNPVPNFGDI TTVTFPVTPN TLPSYQSSPS
460 470 480 490 500
GFYHFGSTSS SPPISPASSD LSAAGSLPDT FTDASPGFGL HASPVPACTD
510 520 530 540 550
ESLLSSLNGG SGPSEPDGLD SEKDKMLVEK QKVINQLTWK LRQEQRQVEE
560 570 580 590 600
LRMQLQKQKS GCNDQKPLPF LATTIKQEDV SSCPFAAQQA SGKGQGHSSD
610 620 630 640 650
SPPPACETAQ LLPHCVESSG QTHVLSSTFL SPQCSPQHSP LGTLKSPQHI
660 670 680 690 700
SLPPSPNNHY FLASSSGAQR ENHGVSSPNS SQGCAQMTGL QSSDKVGPTF
710 720 730 740 750
SIPSPTFPKS SPTVPEITQP PSYEDAVKQQ MTRSQQMDEL LDVLIESGEM
760 770 780 790 800
PADAREDHSC LQKIPKIPGS SCSPTTILPK SSASFEQASS GGQISFDHYA
810 820 830 840 850
TDSEEHLEVL LNSHSPIGKV SDVTLLKIGS EEPPFDGIMD GFPGKAAEDL
860 870 880 890 900
FSAHELLPGP LSPMHTQLSP PSVDSSGLQL SFTESPWETM EWLDLTPPSS
910 920 930
TPGFSNLTSS GPSIFNIDFL DVTDLNLNSP MDLHLQQW
Length:938
Mass (Da):101,872
Last modified:June 27, 2003 - v2
Checksum:iC64F10796AD53618
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF464909 mRNA. Translation: AAL75447.1.
AF464910 mRNA. Translation: AAL75446.1.
AB091378 mRNA. Translation: BAC65150.1.
BK001422 mRNA. Translation: DAA01467.1.
RefSeqiNP_872608.1. NM_182667.2.
UniGeneiRn.163103.

Genome annotation databases

EnsembliENSRNOT00000057562; ENSRNOP00000054372; ENSRNOG00000003669.
GeneIDi246297.
KEGGirno:246297.
UCSCiRGD:631347. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF464909 mRNA. Translation: AAL75447.1.
AF464910 mRNA. Translation: AAL75446.1.
AB091378 mRNA. Translation: BAC65150.1.
BK001422 mRNA. Translation: DAA01467.1.
RefSeqiNP_872608.1. NM_182667.2.
UniGeneiRn.163103.

3D structure databases

ProteinModelPortaliQ8R5I7.
SMRiQ8R5I7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000054372.

Proteomic databases

PaxDbiQ8R5I7.
PRIDEiQ8R5I7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000057562; ENSRNOP00000054372; ENSRNOG00000003669.
GeneIDi246297.
KEGGirno:246297.
UCSCiRGD:631347. rat.

Organism-specific databases

CTDi93649.
RGDi631347. Myocd.

Phylogenomic databases

eggNOGiENOG410IE2C. Eukaryota.
ENOG410XNR1. LUCA.
GeneTreeiENSGT00530000063195.
HOGENOMiHOG000038001.
HOVERGENiHBG036493.
InParanoidiQ8R5I7.
PhylomeDBiQ8R5I7.
TreeFamiTF326024.

Miscellaneous databases

PROiQ8R5I7.

Gene expression databases

BgeeiENSRNOG00000003669.
ExpressionAtlasiQ8R5I7. baseline and differential.
GenevisibleiQ8R5I7. RN.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR028721. MYOCD.
IPR004018. RPEL_repeat.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR22793:SF11. PTHR22793:SF11. 2 hits.
PfamiPF02755. RPEL. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYCD_RAT
AccessioniPrimary (citable) accession number: Q8R5I7
Secondary accession number(s): Q7M6Y4, Q8R5I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: June 27, 2003
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.