Reviewed, UniProtKB/Swiss-Prot Q8R5H1 (UBP15_MOUSE)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 15
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 15
    Ubiquitin-specific-processing protease 15
    Deubiquitinating enzyme 15

Gene names

Name:

Usp15

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	981 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Tissue specificity	Widely expressed with highest levels in testis, heart and liver. Ref.1
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 DUSP domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC ubiquitin-specific protease activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q8R5H1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8R5H1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-25: TLLKTSLR → DAWLKPRSG
     228-256: Missing.

Isoform 3 (identifier: Q8R5H1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     229-981: Missing.

Isoform 4 (identifier: Q8R5H1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     209-227: LVIEQKNEDGTWPRGPSTP → PRCIQFFNFTKDLSFISIK
     228-981: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 981	980	Ubiquitin carboxyl-terminal hydrolase 15		PRO_0000080642
Regions
	Domain	7 – 118	112	DUSP
Sites
	Active site	298	1	By similarity UniProtKB Q9Y4E8
	Active site	883	1	By similarity UniProtKB Q9Y4E8
	Active site	891	1	By similarity UniProtKB Q9Y4E8
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	229	1	Phosphoserine By similarity
	Modified residue	961	1	Phosphoserine By similarity
	Modified residue	965	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	18 – 25	8	TLLKTSLR → DAWLKPRSG in isoform 2. Ref.1		VSP_005262
	Alternative sequence	209 – 227	19	LVIEQ…GPSTP → PRCIQFFNFTKDLSFISIK in isoform 4.		VSP_005265
	Alternative sequence	228 – 981	754	Missing in isoform 4.		VSP_005266
	Alternative sequence	228 – 256	29	Missing in isoform 2. Ref.1		VSP_005263
	Alternative sequence	229 – 981	753	Missing in isoform 3.		VSP_005264

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 6D5377C3FEA6E40A	FASTA	981	112,325
	10 20 30 40 50 60 MAEGGAADLD TQRSDIATLL KTSLRKGDTW YLVDSRWFKQ WKKYVGFDSW DKYQMGDQNV 70 80 90 100 110 120 YPGPIDNSGL LKDGDAQSLK EHLIDELDYI LLPTEGWNKL VSWYTLMEGQ EPIARKVVEQ 130 140 150 160 170 180 GMFVKHCKVE VYLTELKLCE NGNMNNVVTR RFSKADTIDT IEKEIRKIFN IPDEKEARLW 190 200 210 220 230 240 NKYMSNTFEP LNKPDSTIQD AGLYQGQVLV IEQKNEDGTW PRGPSTPKSP GASNFSTLPK 250 260 270 280 290 300 ISPSSLSNNY NNINNRNVKN SNYCLPSYTA YKNYDYSEPG RNNEQPGLCG LSNLGNTCFM 310 320 330 340 350 360 NSAIQCLSNT PPLTEYFLND KYQEELNFDN PLGMRGEIAK SYAELIKQMW SGKFSYVTPR 370 380 390 400 410 420 AFKTQVGRFA PQFSGYQQQD CQELLAFLLD GLHEDLNRIR KKPYIQLKDA DGRPDKVVAE 430 440 450 460 470 480 EAWENHLKRN DSIIVDIFHG LFKSTLVCPE CAKISVTFDP FCYLTLPLPM KKERSLEVYL 490 500 510 520 530 540 VRMDPLAKPM QYKVIVPKIG NILDLCTALS ALSGVPADKM IVTDIYNHRF HRIFAVDENL 550 560 570 580 590 600 SSIMERDDIY VFEININRAE DTEHVVIPVC LREKFRHSSY THHTGSSLFG QPFLMAIPRN 610 620 630 640 650 660 NTEDKLYNLL LLRMCRYVKM STETEETDGH LRCCEDQNIN GNGPNGLHEE GSPSEMETDE 670 680 690 700 710 720 PDDESSQDQE LPSENENSQS EDSVGGDNDS ENGLCTEETC KGQLTGHKKR LFTFQFNNLG 730 740 750 760 770 780 NNDINYIKDD TSHIRFDDRQ LRLDERSFLA LDWDPDLKKR YFDENAAEDF EKHESVEYKP 790 800 810 820 830 840 PKRPFVKLKD CIELFTTKEK LGAEDPWYCP NCKEHQQATK KLDLWSLPPV LVVHLKRFSY 850 860 870 880 890 900 SRYMRDKLDT LVDFPISDLD MSEFLINPNA GPCRYNLIAV SNHYGGMGGG HYTAFAKNKD 910 920 930 940 950 960 DGKWYYFDDS SVSTASEDQI VSKAAYVLFY QRQDTFSGTG FFPLDRETKG ASAATGIPLE 970 980 SDEDSNDNDN DLENENCMHT N
	Isoform 2. Checksum: D56685B92C029188	FASTA	953	109,318
	10 20 30 40 50 60 MAEGGAADLD TQRSDIADAW LKPRSGKGDT WYLVDSRWFK QWKKYVGFDS WDKYQMGDQN 70 80 90 100 110 120 VYPGPIDNSG LLKDGDAQSL KEHLIDELDY ILLPTEGWNK LVSWYTLMEG QEPIARKVVE 130 140 150 160 170 180 QGMFVKHCKV EVYLTELKLC ENGNMNNVVT RRFSKADTID TIEKEIRKIF NIPDEKEARL 190 200 210 220 230 240 WNKYMSNTFE PLNKPDSTIQ DAGLYQGQVL VIEQKNEDGT WPRGPSTPNV KNSNYCLPSY 250 260 270 280 290 300 TAYKNYDYSE PGRNNEQPGL CGLSNLGNTC FMNSAIQCLS NTPPLTEYFL NDKYQEELNF 310 320 330 340 350 360 DNPLGMRGEI AKSYAELIKQ MWSGKFSYVT PRAFKTQVGR FAPQFSGYQQ QDCQELLAFL 370 380 390 400 410 420 LDGLHEDLNR IRKKPYIQLK DADGRPDKVV AEEAWENHLK RNDSIIVDIF HGLFKSTLVC 430 440 450 460 470 480 PECAKISVTF DPFCYLTLPL PMKKERSLEV YLVRMDPLAK PMQYKVIVPK IGNILDLCTA 490 500 510 520 530 540 LSALSGVPAD KMIVTDIYNH RFHRIFAVDE NLSSIMERDD IYVFEININR AEDTEHVVIP 550 560 570 580 590 600 VCLREKFRHS SYTHHTGSSL FGQPFLMAIP RNNTEDKLYN LLLLRMCRYV KMSTETEETD 610 620 630 640 650 660 GHLRCCEDQN INGNGPNGLH EEGSPSEMET DEPDDESSQD QELPSENENS QSEDSVGGDN 670 680 690 700 710 720 DSENGLCTEE TCKGQLTGHK KRLFTFQFNN LGNNDINYIK DDTSHIRFDD RQLRLDERSF 730 740 750 760 770 780 LALDWDPDLK KRYFDENAAE DFEKHESVEY KPPKRPFVKL KDCIELFTTK EKLGAEDPWY 790 800 810 820 830 840 CPNCKEHQQA TKKLDLWSLP PVLVVHLKRF SYSRYMRDKL DTLVDFPISD LDMSEFLINP 850 860 870 880 890 900 NAGPCRYNLI AVSNHYGGMG GGHYTAFAKN KDDGKWYYFD DSSVSTASED QIVSKAAYVL 910 920 930 940 950 FYQRQDTFSG TGFFPLDRET KGASAATGIP LESDEDSNDN DNDLENENCM HTN
	Isoform 3. Checksum: 43F1F4F48122703B	FASTA	228	26,305
	10 20 30 40 50 60 MAEGGAADLD TQRSDIATLL KTSLRKGDTW YLVDSRWFKQ WKKYVGFDSW DKYQMGDQNV 70 80 90 100 110 120 YPGPIDNSGL LKDGDAQSLK EHLIDELDYI LLPTEGWNKL VSWYTLMEGQ EPIARKVVEQ 130 140 150 160 170 180 GMFVKHCKVE VYLTELKLCE NGNMNNVVTR RFSKADTIDT IEKEIRKIFN IPDEKEARLW 190 200 210 220 NKYMSNTFEP LNKPDSTIQD AGLYQGQVLV IEQKNEDGTW PRGPSTPK
	Isoform 4. Checksum: A92B80D4BEE7E26E	FASTA	227	26,357
	10 20 30 40 50 60 MAEGGAADLD TQRSDIATLL KTSLRKGDTW YLVDSRWFKQ WKKYVGFDSW DKYQMGDQNV 70 80 90 100 110 120 YPGPIDNSGL LKDGDAQSLK EHLIDELDYI LLPTEGWNKL VSWYTLMEGQ EPIARKVVEQ 130 140 150 160 170 180 GMFVKHCKVE VYLTELKLCE NGNMNNVVTR RFSKADTIDT IEKEIRKIFN IPDEKEARLW 190 200 210 220 NKYMSNTFEP LNKPDSTIQD AGLYQGQVPR CIQFFNFTKD LSFISIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the mouse ubiquitin-specific protease Usp15." Angelats C., Wang X.-W., Jermiin L.S., Copeland N.G., Jenkins N.A., Baker R.T. Mamm. Genome 14:31-46(2003) [PubMed: 12532266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4). Strain: C57BL/6J. Tissue: Corpora quadrigemina, Testis and Thymus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF468037 mRNA. Translation: AAL77418.1. AK046332 mRNA. Translation: BAC32683.1. AK083303 mRNA. Translation: BAC38854.1. AK145749 mRNA. Translation: BAE26626.1.
IPI	IPI00154012. IPI00222152. IPI00222154. IPI00403342.
RefSeq	NP_081880.2.
UniGene	Mm.244209 Mm.470032
3D structure databases
HSSP	HSSP built from PDB template 1NB8 based on UniProtKB Q93009.
SMR	Q8R5H1. Positions 1-120.
ModBase	Search...
PTM databases
PhosphoSite	Q8R5H1.
Proteomic databases
PRIDE	Q8R5H1.
Genome annotation databases
Ensembl	ENSMUSG00000020124. Mus musculus. [Contig view]
GeneID	14479.
KEGG	mmu:14479.
Organism-specific databases
MGI	MGI:101857. Usp15.
Phylogenomic databases
HOGENOM	Q8R5H1.
HOVERGEN	Q8R5H1.
OMA	Q8R5H1. LCTEETC.
Enzyme and pathway databases
BRENDA	3.1.2.15. 244.
Gene expression databases
ArrayExpress	Q8R5H1.
Bgee	Q8R5H1.
CleanEx	MM_USP15.
GermOnline	ENSMUSG00000020124. Mus musculus.
Family and domain databases
InterPro	IPR006615. Pept_C19_DUSP. IPR010460. Pept_C19_N_2. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF06337. DUF1055. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00695. DUSP. 1 hit. [Graphical view]
PROSITE	PS51283. DUSP. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	286150.
SOURCE	Search...

Entry information

Entry name

UBP15_MOUSE

Accession

Primary (citable) accession number: Q8R5H1
Secondary accession number(s): Q3UL25

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)