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Protein

Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3

Gene

Arap3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts preferentially on ARF5 and on RHOA.1 Publication

GO - Molecular functioni

GO - Biological processi

  • negative regulation of cell migration Source: MGI
  • negative regulation of Rac protein signal transduction Source: MGI
  • negative regulation of Rho protein signal transduction Source: MGI
  • regulation of cell shape Source: MGI
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3
Alternative name(s):
Centaurin-delta-3
Short name:
Cnt-d3
Dual specificity Rho- and Arf-GTPase-activating protein 1
Gene namesi
Name:Arap3
Synonyms:Centd3, Drag1, Kiaa4097
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2147274. Arap3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • lamellipodium Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
  • ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi938R → L: Loss of Rho GAP activity. Enhances cell spreading. 1 Publication1
Mutagenesisi1399Y → F: Strongly reduces phosphorylation by CSK; when associated with F-1404. 1 Publication1
Mutagenesisi1404Y → F: Strongly reduces phosphorylation by CSK; when associated with F-1399. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742161 – 1538Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 3Add BLAST1538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1344PhosphothreonineCombined sources1
Modified residuei1399Phosphotyrosine1 Publication1
Modified residuei1404PhosphotyrosineCombined sources1 Publication1
Modified residuei1438PhosphoserineBy similarity1
Modified residuei1474PhosphoserineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated at a low basal level. PDGF treatment stimulates phosphorylation. Tyrosine phosphorylation is increased in cells that are in the process of becoming attached to a substrate and that start spreading and flattening.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8R5G7.
PRIDEiQ8R5G7.

PTM databases

iPTMnetiQ8R5G7.
PhosphoSitePlusiQ8R5G7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024451.
GenevisibleiQ8R5G7. MM.

Interactioni

Subunit structurei

Interacts (via SAM domain) with INPPL1/SHIP2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
LYNP079482EBI-621463,EBI-79452From a different organism.
SRCP129313EBI-621463,EBI-621482From a different organism.

Protein-protein interaction databases

IntActiQ8R5G7. 3 interactors.
STRINGi10090.ENSMUSP00000035662.

Structurei

3D structure databases

ProteinModelPortaliQ8R5G7.
SMRiQ8R5G7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 68SAMPROSITE-ProRule annotationAdd BLAST65
Domaini282 – 374PH 1PROSITE-ProRule annotationAdd BLAST93
Domaini389 – 478PH 2PROSITE-ProRule annotationAdd BLAST90
Domaini479 – 606Arf-GAPPROSITE-ProRule annotationAdd BLAST128
Domaini671 – 785PH 3PROSITE-ProRule annotationAdd BLAST115
Domaini795 – 901PH 4PROSITE-ProRule annotationAdd BLAST107
Domaini903 – 1084Rho-GAPPROSITE-ProRule annotationAdd BLAST182
Domaini1113 – 1206Ras-associatingPROSITE-ProRule annotationAdd BLAST94
Domaini1219 – 1321PH 5PROSITE-ProRule annotationAdd BLAST103

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1493 – 1536Pro-richAdd BLAST44

Sequence similaritiesi

Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 5 PH domains.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1117. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000246988.
HOVERGENiHBG069114.
InParanoidiQ8R5G7.
KOiK12490.
OMAiHEGIYRK.
OrthoDBiEOG091G00AN.
TreeFamiTF105769.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.10.555.10. 1 hit.
2.30.29.30. 3 hits.
InterProiIPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
PF00169. PH. 2 hits.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 5 hits.
SM00324. RhoGAP. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 5 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 3 hits.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R5G7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPQDLDIA VWLALVHLEQ YADTFRRHGL ATAGAAQHLG HEELRHLGIS
60 70 80 90 100
ATGHRKRILR LLRAGSAEGF LDSHLDNTME PTPSPAPDAQ PPKPVPKPRT
110 120 130 140 150
VFGLSNPATA QRPGLSPIFW DPEVSRNSEC TQRSSPLLPS SSEQPSVPNT
160 170 180 190 200
MEMMPNAIYF GLDLRGRAQA AQDVTPDSSQ ATVPTPAFRP TTGTVHIMDP
210 220 230 240 250
GCLYYGVQPV GIPGASDRRD GRGVCQERAE HRQDLETRED AGYASLELPG
260 270 280 290 300
DSILSLPTQD AETSDDLISP YASFSSTADR PVPLLSGWLD KLSPQGNYVF
310 320 330 340 350
QRRFVQFNGR SLMYFGSDKD PFPKGVIPLT AIEMTRSSKD NKFQVITGQR
360 370 380 390 400
VFVFRTESEA QRDLWCSTLQ SCLKEQRLLG HPRPPHPPRP LRTGTLELRG
410 420 430 440 450
HKAKVFAALI PGELALYKSE QAFSLGIGIC FIELQGCSVR ETKSRSFDLL
460 470 480 490 500
TPHRCFSFTA ESGGARQSWA AALQEAVTET LSDYEVAEKV WSNPANRHCA
510 520 530 540 550
DCRASRPDWA AVNLGVVICK QCAGQHRALG SGISKVQSLK LDTSVWSNEI
560 570 580 590 600
VQLFIVLGND RANCFWAGAL PPGEGLHPDS APGPRGEFIS RKYKLGLFRK
610 620 630 640 650
PHPRHPDHSQ LLQALCAAMA GPNLLKNMAQ LLCVETSEGE EPLSPSALNG
660 670 680 690 700
SLLSLLPSDS PGVYNEVVVP ATYRGFLYCG SISNKAGAPP LRRGRDAPPR
710 720 730 740 750
LWCVLGAALE MFASESSPEP LSLLQPQDIV CLGVSPPPAD PGDLDRFPFS
760 770 780 790 800
FELILTGGRI QHFATDGADS LEAWISAVGK WFSPLSCHQL LGPGLLRMGR
810 820 830 840 850
LWLRSPSHAG LAPGLWLSGF GLLRGDHLFL CPAPGPGPPA PEDMVHLRRL
860 870 880 890 900
QEISVVSAAD TPDKKEHLVL VETGRTLYLQ GEGRLDFAAW NTAIGGAAGG
910 920 930 940 950
GGTGLQEQQM SRGDIPIIVD ACISFVTQHG LRLEGVYRKG GARARSLRLL
960 970 980 990 1000
AEFRRDARSV KLRPREHFVE DVTDTLKRFF RELDDPVTSA RLLPRWREAA
1010 1020 1030 1040 1050
ELSQKNQRLE KYKEVISCLP RVNRRTLATL IGHLYRVQKC ASLNQMCTRN
1060 1070 1080 1090 1100
LALLFAPSVF QTDGRGEHEV RVLQELIDGY ISVFDIDSDQ AAQIDLEVSL
1110 1120 1130 1140 1150
ITTWKDVQLS QAGDLIMEVY IEQQLPDNCV TLKVSPTLTA EELTNQVLEM
1160 1170 1180 1190 1200
RGAASGTDLW VTFEILEHGE LERPLHPKEK VLEQALQWCQ LPEPCSASLL
1210 1220 1230 1240 1250
LRKVSMAHAG CLFTGVRRES PRVGLLRCRE EPPRLLGNRF QERFFLVRGR
1260 1270 1280 1290 1300
CLLLLKEKKS SKPEREWSLE GAKVYLGIRK KLKPPTLWGF TLILEKMHLC
1310 1320 1330 1340 1350
LSCMDEEEMW DWTTSILKAQ HDDQQSVVLR RRSSSDLARQ KFGTMPLLPI
1360 1370 1380 1390 1400
RGDDSGATLL SANQTLRRLH NRRTLSMFFP MKSPQGSVEE QDELEEPVYE
1410 1420 1430 1440 1450
EPVYEEVGAF PELTKDTTFS STWEWSAKSD PSLTSQRSFD QPPLSKASML
1460 1470 1480 1490 1500
GHEERIPDPP PGPPSKSSSQ ARGSLEEQLL QELNNLILRK GEPASCPESS
1510 1520 1530
SQPTSPQAPS PTSLPTPTPS LPTQPPCTSN PPSSQPLT
Length:1,538
Mass (Da):169,741
Last modified:July 27, 2011 - v3
Checksum:iD7E55C57FD4B83ED
GO
Isoform 2 (identifier: Q8R5G7-2) [UniParc]FASTAAdd to basket
Also known as: ARAP3 delta-SAM

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Show »
Length:1,460
Mass (Da):161,171
Checksum:iC00130DB36CA8116
GO
Isoform 3 (identifier: Q8R5G7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-618: Missing.
     1446-1538: KASMLGHEER...SNPPSSQPLT → GPSPFVCTMKSLKEGEG

Note: No experimental confirmation available.
Show »
Length:844
Mass (Da):94,043
Checksum:i5742FA191BD0F796
GO

Sequence cautioni

The sequence AAL78678 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD90507 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144Q → H in AAH68145 (PubMed:15489334).Curated1
Sequence conflicti167R → Q in AAL78677 (PubMed:15546919).Curated1
Sequence conflicti167R → Q in AAL78678 (PubMed:15546919).Curated1
Sequence conflicti1304M → T in AAL78677 (PubMed:15546919).Curated1
Sequence conflicti1304M → T in AAL78678 (PubMed:15546919).Curated1
Sequence conflicti1500S → P in AAL78677 (PubMed:15546919).Curated1
Sequence conflicti1500S → P in AAL78678 (PubMed:15546919).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0150021 – 618Missing in isoform 3. 1 PublicationAdd BLAST618
Alternative sequenceiVSP_0150031 – 78Missing in isoform 2. 1 PublicationAdd BLAST78
Alternative sequenceiVSP_0150041446 – 1538KASML…SQPLT → GPSPFVCTMKSLKEGEG in isoform 3. 1 PublicationAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF469621 mRNA. Translation: AAL78677.1.
AF469622 mRNA. Translation: AAL78678.1. Different initiation.
AC121886 Genomic DNA. No translation available.
AC129315 Genomic DNA. No translation available.
BC068145 mRNA. Translation: AAH68145.1.
AK220486 mRNA. Translation: BAD90507.1. Different initiation.
CCDSiCCDS29196.1. [Q8R5G7-1]
RefSeqiNP_001192265.1. NM_001205336.1. [Q8R5G7-2]
NP_631945.2. NM_139206.2. [Q8R5G7-1]
XP_006525552.1. XM_006525489.1. [Q8R5G7-1]
UniGeneiMm.247642.

Genome annotation databases

EnsembliENSMUST00000042944; ENSMUSP00000035662; ENSMUSG00000024451. [Q8R5G7-1]
GeneIDi106952.
KEGGimmu:106952.
UCSCiuc008erq.2. mouse. [Q8R5G7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF469621 mRNA. Translation: AAL78677.1.
AF469622 mRNA. Translation: AAL78678.1. Different initiation.
AC121886 Genomic DNA. No translation available.
AC129315 Genomic DNA. No translation available.
BC068145 mRNA. Translation: AAH68145.1.
AK220486 mRNA. Translation: BAD90507.1. Different initiation.
CCDSiCCDS29196.1. [Q8R5G7-1]
RefSeqiNP_001192265.1. NM_001205336.1. [Q8R5G7-2]
NP_631945.2. NM_139206.2. [Q8R5G7-1]
XP_006525552.1. XM_006525489.1. [Q8R5G7-1]
UniGeneiMm.247642.

3D structure databases

ProteinModelPortaliQ8R5G7.
SMRiQ8R5G7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R5G7. 3 interactors.
STRINGi10090.ENSMUSP00000035662.

PTM databases

iPTMnetiQ8R5G7.
PhosphoSitePlusiQ8R5G7.

Proteomic databases

PaxDbiQ8R5G7.
PRIDEiQ8R5G7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042944; ENSMUSP00000035662; ENSMUSG00000024451. [Q8R5G7-1]
GeneIDi106952.
KEGGimmu:106952.
UCSCiuc008erq.2. mouse. [Q8R5G7-1]

Organism-specific databases

CTDi64411.
MGIiMGI:2147274. Arap3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1117. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00860000133715.
HOGENOMiHOG000246988.
HOVERGENiHBG069114.
InParanoidiQ8R5G7.
KOiK12490.
OMAiHEGIYRK.
OrthoDBiEOG091G00AN.
TreeFamiTF105769.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ8R5G7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024451.
GenevisibleiQ8R5G7. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.10.555.10. 1 hit.
2.30.29.30. 3 hits.
InterProiIPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF01412. ArfGap. 1 hit.
PF00169. PH. 2 hits.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00105. ArfGap. 1 hit.
SM00233. PH. 5 hits.
SM00324. RhoGAP. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 5 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 3 hits.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARAP3_MOUSE
AccessioniPrimary (citable) accession number: Q8R5G7
Secondary accession number(s): E9QMK7
, Q5DTN4, Q6NVF1, Q8R5G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.