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Protein

Adenine DNA glycosylase

Gene

Mutyh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.By similarity

Catalytic activityi

Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105Proton donor/acceptorBy similarity1
Sitei207Transition state stabilizerBy similarity1
Metal bindingi261Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi268Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi271Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi277Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: InterPro
  • response to oxidative stress Source: RGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine DNA glycosylase (EC:3.2.2.31By similarity)
Alternative name(s):
MutY homolog
Short name:
rMYH
Gene namesi
Name:Mutyh
Synonyms:Myh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620045 Mutyh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022411 – 516Adenine DNA glycosylaseAdd BLAST516

Proteomic databases

PaxDbiQ8R5G2
PRIDEiQ8R5G2

PTM databases

PhosphoSitePlusiQ8R5G2

Expressioni

Tissue specificityi

Expressed in brain, spleen, heart, liver and kidney.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024375

Structurei

3D structure databases

ProteinModelPortaliQ8R5G2
SMRiQ8R5G2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini335 – 467Nudix hydrolasePROSITE-ProRule annotationAdd BLAST133

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi376 – 398Nudix boxAdd BLAST23

Sequence similaritiesi

Belongs to the Nth/MutY family.Curated

Phylogenomic databases

eggNOGiKOG2457 Eukaryota
COG1194 LUCA
HOGENOMiHOG000028743
HOVERGENiHBG052540
InParanoidiQ8R5G2
KOiK03575
PhylomeDBiQ8R5G2

Family and domain databases

CDDicd03431 DNA_Glycosylase_C, 1 hit
cd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
InterProiView protein in InterPro
IPR005760 A/G_AdeGlyc_MutY
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR029119 MutY_C
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
PF14815 NUDIX_4, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
SSF55811 SSF55811, 1 hit
TIGRFAMsiTIGR01084 mutY, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit
PS51462 NUDIX, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R5G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLRASVRS HKKQPANHKR RGKCALSSSQ AKPSGLDGLA KQKREELLKT
60 70 80 90 100
PVSPYHLFSD IADVTAFRRN LLSWYDQEKR DLPWRKRVKE ETNLDRRAYA
110 120 130 140 150
VWVSEVMLQQ TQVATVIDYY TRWMQKWPTL QDLASASLEE VNQLWSGLGY
160 170 180 190 200
YSRGRRLQEG ARKVVEELGG HVPRTAETLQ QLLPGVGRYT AGAIASIAFD
210 220 230 240 250
QVTGVVDGNV IRVLCRVRAI GADPTSSFVS HHLWDLAQQL VDPARPGDFN
260 270 280 290 300
QAAMELGATV CTPQRPLCNH CPVQSLCRAH QRVGQGRLSA LPGSPDIEEC
310 320 330 340 350
ALNTRQCQLC LPSTNPWDPN MGVVNFPRKA SRRPPREEYS ATCVVEQPGA
360 370 380 390 400
TGGPLILLVQ RPNSGLLAGL WEFPSVTLEP SGQHQHKALL QELQHWSAPL
410 420 430 440 450
PTTPLQHLGE VIHVFSHIKL TYQVYSLALE GQTPASTTLP GARWLTWEEF
460 470 480 490 500
RNAAVSTAMK KVFRVYEEHR RGTCKGSKRP QVCTPSSRKK PSRGQQVLDR
510
FFQRHIPTHK PNSTTQ
Length:516
Mass (Da):57,919
Last modified:June 1, 2002 - v1
Checksum:iE42FD4AC86BE3467
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF478683 mRNA Translation: AAL79551.1
RefSeqiNP_579850.1, NM_133316.1
UniGeneiRn.44045

Genome annotation databases

GeneIDi170841
KEGGirno:170841
UCSCiRGD:620045 rat

Similar proteinsi

Entry informationi

Entry nameiMUTYH_RAT
AccessioniPrimary (citable) accession number: Q8R5G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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