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Protein

Epidermal growth factor receptor kinase substrate 8-like protein 1

Gene

Eps8l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 1
Short name:
EPS8-like protein 1
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 1
Short name:
EPS8-related protein 1
Gene namesi
Name:Eps8l1
Synonyms:Eps8r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1914675. Eps8l1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Epidermal growth factor receptor kinase substrate 8-like protein 1PRO_0000239083Add
BLAST

Proteomic databases

PaxDbiQ8R5F8.
PRIDEiQ8R5F8.

PTM databases

PhosphoSiteiQ8R5F8.

Expressioni

Tissue specificityi

Detected in placenta, skin, mammary gland, bone marrow and stomach.1 Publication

Gene expression databases

BgeeiQ8R5F8.
CleanExiMM_EPS8L1.
ExpressionAtlasiQ8R5F8. baseline and differential.
GenevisibleiQ8R5F8. MM.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083559.

Structurei

Secondary structure

1
716
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 4514Combined sources
Beta strandi48 – 503Combined sources
Helixi55 – 6713Combined sources
Beta strandi74 – 807Combined sources
Beta strandi82 – 887Combined sources
Turni90 – 923Combined sources
Beta strandi95 – 1006Combined sources
Helixi101 – 1033Combined sources
Beta strandi104 – 1107Combined sources
Beta strandi119 – 1246Combined sources
Beta strandi133 – 1397Combined sources
Turni140 – 1423Combined sources
Helixi143 – 15816Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CY4X-ray1.94A26-165[»]
2CY5X-ray1.90A26-165[»]
ProteinModelPortaliQ8R5F8.
SMRiQ8R5F8. Positions 30-159, 478-536, 605-687.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R5F8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini477 – 53660SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili682 – 71332Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi119 – 1224Poly-Leu
Compositional biasi554 – 56411Poly-ProAdd
BLAST

Sequence similaritiesi

Belongs to the EPS8 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG263108.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ8R5F8.
KOiK17277.
PhylomeDBiQ8R5F8.
TreeFamiTF313069.

Family and domain databases

InterProiIPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R5F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTTGPEAA PKPSAKSIYE QRKRYSTVVM ADVSQYHVNH LVTFCLGEED
60 70 80 90 100
GVHTVEDASR KLAVMDSQGR VWAQEMLLRV SPSQVTLLDP VSKEELESYP
110 120 130 140 150
LDAIVRCDAV MPRGRSRSLL LLVCQEPERA QPDVHFFQGL LLGAELIRED
160 170 180 190 200
IQGALQNYRS GRGERRAAAL RATQEELRRG ASPAAETPPL QRRPSVRLVI
210 220 230 240 250
NTVEPSAVRG RPQVESIPET EEARKPDQAR TTSSADPTSP DLGPRGPELA
260 270 280 290 300
GLQAERDVDI LNHVFDDVES FVSRLQKSAE ATRVLEHRER GRRTRRRAAG
310 320 330 340 350
EGLLTLRAKP PTEAEYTDVL QKIKYAFSLL ARLRGNIANP SSPELLHFLF
360 370 380 390 400
GPLQMIVNTS GGPEFAKSVR RPHLTLEAVT LLRDNVTPGE NELWTSLGDS
410 420 430 440 450
WTCPGVELPP EEGSPYSPEF YNGWEPPATD PQGRPWEDPV EKQLQHEKRR
460 470 480 490 500
RQQSAPQVAV NGQQDPELET ESQLEEKARK WVLCNYDFQA RNGSELSVKH
510 520 530 540 550
RDVLEVLDDR RKWWKVRDHQ GQEGYVPYNI LTPHPGPQVH RSQSPARHLE
560 570 580 590 600
TSTPPPPPAP APAPTQVRPQ WDSCDSLNSL DPSEKEKFSQ MLCVNEELQS
610 620 630 640 650
RLAQGRSGPS RVTPGPRAQE PQLSPRSEAS VVRAWLQTKG FSSGTVEALG
660 670 680 690 700
VLTGAQLFSL QKEELRAVCP EEGARVYSQV TVQRALLEDR EKVSELEAVM
710
EKQKKKVEGE TKTEVI
Length:716
Mass (Da):80,028
Last modified:June 1, 2002 - v1
Checksum:iE996E664722E885D
GO

Sequence cautioni

The sequence BC027043 differs from that shown. Reason: Frameshift at position 463. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti464 – 4641Missing in BC027043 (PubMed:15489334).Curated
Sequence conflicti601 – 6011R → S in BC027043 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074931 mRNA. Translation: AAL76120.1.
AK019490 mRNA. Translation: BAB31756.2.
BC027043 mRNA. No translation available.
CCDSiCCDS20736.1.
RefSeqiNP_001277345.1. NM_001290416.1.
NP_080422.1. NM_026146.4.
XP_006540387.1. XM_006540324.2.
XP_006540388.1. XM_006540325.2.
XP_006540389.1. XM_006540326.1.
XP_006540390.1. XM_006540327.2.
XP_011248986.1. XM_011250684.1.
UniGeneiMm.360392.

Genome annotation databases

EnsembliENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.
GeneIDi67425.
KEGGimmu:67425.
UCSCiuc009exo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074931 mRNA. Translation: AAL76120.1.
AK019490 mRNA. Translation: BAB31756.2.
BC027043 mRNA. No translation available.
CCDSiCCDS20736.1.
RefSeqiNP_001277345.1. NM_001290416.1.
NP_080422.1. NM_026146.4.
XP_006540387.1. XM_006540324.2.
XP_006540388.1. XM_006540325.2.
XP_006540389.1. XM_006540326.1.
XP_006540390.1. XM_006540327.2.
XP_011248986.1. XM_011250684.1.
UniGeneiMm.360392.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CY4X-ray1.94A26-165[»]
2CY5X-ray1.90A26-165[»]
ProteinModelPortaliQ8R5F8.
SMRiQ8R5F8. Positions 30-159, 478-536, 605-687.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083559.

PTM databases

PhosphoSiteiQ8R5F8.

Proteomic databases

PaxDbiQ8R5F8.
PRIDEiQ8R5F8.

Protocols and materials databases

DNASUi67425.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.
GeneIDi67425.
KEGGimmu:67425.
UCSCiuc009exo.2. mouse.

Organism-specific databases

CTDi54869.
MGIiMGI:1914675. Eps8l1.

Phylogenomic databases

eggNOGiNOG263108.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ8R5F8.
KOiK17277.
PhylomeDBiQ8R5F8.
TreeFamiTF313069.

Miscellaneous databases

EvolutionaryTraceiQ8R5F8.
NextBioi324528.
PROiQ8R5F8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8R5F8.
CleanExiMM_EPS8L1.
ExpressionAtlasiQ8R5F8. baseline and differential.
GenevisibleiQ8R5F8. MM.

Family and domain databases

InterProiIPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure."
    Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C.
    Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  4. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
    Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
    Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiES8L1_MOUSE
AccessioniPrimary (citable) accession number: Q8R5F8
Secondary accession number(s): Q8R0D6, Q9D2M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2002
Last modified: July 22, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.