Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8R5F8

- ES8L1_MOUSE

UniProt

Q8R5F8 - ES8L1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Epidermal growth factor receptor kinase substrate 8-like protein 1

Gene

Eps8l1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.By similarity

GO - Molecular functioni

  1. actin filament binding Source: MGI
  2. Rac guanyl-nucleotide exchange factor activity Source: Ensembl

GO - Biological processi

  1. positive regulation of ruffle assembly Source: Ensembl
  2. Rac protein signal transduction Source: MGI
  3. regulation of Rac GTPase activity Source: Ensembl
  4. Rho protein signal transduction Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 1
Short name:
EPS8-like protein 1
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 1
Short name:
EPS8-related protein 1
Gene namesi
Name:Eps8l1
Synonyms:Eps8r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1914675. Eps8l1.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. protein complex Source: Ensembl
  4. ruffle Source: MGI
  5. ruffle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Epidermal growth factor receptor kinase substrate 8-like protein 1PRO_0000239083Add
BLAST

Proteomic databases

PaxDbiQ8R5F8.
PRIDEiQ8R5F8.

PTM databases

PhosphoSiteiQ8R5F8.

Expressioni

Tissue specificityi

Detected in placenta, skin, mammary gland, bone marrow and stomach.1 Publication

Gene expression databases

BgeeiQ8R5F8.
CleanExiMM_EPS8L1.
ExpressionAtlasiQ8R5F8. baseline and differential.
GenevestigatoriQ8R5F8.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083559.

Structurei

Secondary structure

1
716
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 4514
Beta strandi48 – 503
Helixi55 – 6713
Beta strandi74 – 807
Beta strandi82 – 887
Turni90 – 923
Beta strandi95 – 1006
Helixi101 – 1033
Beta strandi104 – 1107
Beta strandi119 – 1246
Beta strandi133 – 1397
Turni140 – 1423
Helixi143 – 15816

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CY4X-ray1.94A26-165[»]
2CY5X-ray1.90A26-165[»]
ProteinModelPortaliQ8R5F8.
SMRiQ8R5F8. Positions 30-159, 478-536, 605-687.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R5F8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini477 – 53660SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili682 – 71332Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi119 – 1224Poly-Leu
Compositional biasi554 – 56411Poly-ProAdd
BLAST

Sequence similaritiesi

Belongs to the EPS8 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG263108.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ8R5F8.
KOiK17277.
PhylomeDBiQ8R5F8.
TreeFamiTF313069.

Family and domain databases

InterProiIPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R5F8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTTTGPEAA PKPSAKSIYE QRKRYSTVVM ADVSQYHVNH LVTFCLGEED
60 70 80 90 100
GVHTVEDASR KLAVMDSQGR VWAQEMLLRV SPSQVTLLDP VSKEELESYP
110 120 130 140 150
LDAIVRCDAV MPRGRSRSLL LLVCQEPERA QPDVHFFQGL LLGAELIRED
160 170 180 190 200
IQGALQNYRS GRGERRAAAL RATQEELRRG ASPAAETPPL QRRPSVRLVI
210 220 230 240 250
NTVEPSAVRG RPQVESIPET EEARKPDQAR TTSSADPTSP DLGPRGPELA
260 270 280 290 300
GLQAERDVDI LNHVFDDVES FVSRLQKSAE ATRVLEHRER GRRTRRRAAG
310 320 330 340 350
EGLLTLRAKP PTEAEYTDVL QKIKYAFSLL ARLRGNIANP SSPELLHFLF
360 370 380 390 400
GPLQMIVNTS GGPEFAKSVR RPHLTLEAVT LLRDNVTPGE NELWTSLGDS
410 420 430 440 450
WTCPGVELPP EEGSPYSPEF YNGWEPPATD PQGRPWEDPV EKQLQHEKRR
460 470 480 490 500
RQQSAPQVAV NGQQDPELET ESQLEEKARK WVLCNYDFQA RNGSELSVKH
510 520 530 540 550
RDVLEVLDDR RKWWKVRDHQ GQEGYVPYNI LTPHPGPQVH RSQSPARHLE
560 570 580 590 600
TSTPPPPPAP APAPTQVRPQ WDSCDSLNSL DPSEKEKFSQ MLCVNEELQS
610 620 630 640 650
RLAQGRSGPS RVTPGPRAQE PQLSPRSEAS VVRAWLQTKG FSSGTVEALG
660 670 680 690 700
VLTGAQLFSL QKEELRAVCP EEGARVYSQV TVQRALLEDR EKVSELEAVM
710
EKQKKKVEGE TKTEVI
Length:716
Mass (Da):80,028
Last modified:June 1, 2002 - v1
Checksum:iE996E664722E885D
GO

Sequence cautioni

The sequence BC027043 differs from that shown. Reason: Frameshift at position 463.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti464 – 4641Missing in BC027043. (PubMed:15489334)Curated
Sequence conflicti601 – 6011R → S in BC027043. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY074931 mRNA. Translation: AAL76120.1.
AK019490 mRNA. Translation: BAB31756.2.
BC027043 mRNA. No translation available.
CCDSiCCDS20736.1.
RefSeqiNP_001277345.1. NM_001290416.1.
NP_080422.1. NM_026146.4.
XP_006540387.1. XM_006540324.1.
XP_006540388.1. XM_006540325.1.
XP_006540389.1. XM_006540326.1.
XP_006540390.1. XM_006540327.1.
UniGeneiMm.360392.

Genome annotation databases

EnsembliENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.
GeneIDi67425.
KEGGimmu:67425.
UCSCiuc009exo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY074931 mRNA. Translation: AAL76120.1 .
AK019490 mRNA. Translation: BAB31756.2 .
BC027043 mRNA. No translation available.
CCDSi CCDS20736.1.
RefSeqi NP_001277345.1. NM_001290416.1.
NP_080422.1. NM_026146.4.
XP_006540387.1. XM_006540324.1.
XP_006540388.1. XM_006540325.1.
XP_006540389.1. XM_006540326.1.
XP_006540390.1. XM_006540327.1.
UniGenei Mm.360392.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CY4 X-ray 1.94 A 26-165 [» ]
2CY5 X-ray 1.90 A 26-165 [» ]
ProteinModelPortali Q8R5F8.
SMRi Q8R5F8. Positions 30-159, 478-536, 605-687.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000083559.

PTM databases

PhosphoSitei Q8R5F8.

Proteomic databases

PaxDbi Q8R5F8.
PRIDEi Q8R5F8.

Protocols and materials databases

DNASUi 67425.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000086372 ; ENSMUSP00000083559 ; ENSMUSG00000006154 .
ENSMUST00000163893 ; ENSMUSP00000125840 ; ENSMUSG00000006154 .
GeneIDi 67425.
KEGGi mmu:67425.
UCSCi uc009exo.1. mouse.

Organism-specific databases

CTDi 54869.
MGIi MGI:1914675. Eps8l1.

Phylogenomic databases

eggNOGi NOG263108.
GeneTreei ENSGT00390000003646.
HOGENOMi HOG000060324.
HOVERGENi HBG003090.
InParanoidi Q8R5F8.
KOi K17277.
PhylomeDBi Q8R5F8.
TreeFami TF313069.

Miscellaneous databases

EvolutionaryTracei Q8R5F8.
NextBioi 324528.
PROi Q8R5F8.
SOURCEi Search...

Gene expression databases

Bgeei Q8R5F8.
CleanExi MM_EPS8L1.
ExpressionAtlasi Q8R5F8. baseline and differential.
Genevestigatori Q8R5F8.

Family and domain databases

InterProi IPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure."
    Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C.
    Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  4. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
    Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
    Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiES8L1_MOUSE
AccessioniPrimary (citable) accession number: Q8R5F8
Secondary accession number(s): Q8R0D6, Q9D2M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3