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Q8R5F8

- ES8L1_MOUSE

UniProt

Q8R5F8 - ES8L1_MOUSE

Protein

Epidermal growth factor receptor kinase substrate 8-like protein 1

Gene

Eps8l1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.By similarity

    GO - Molecular functioni

    1. actin filament binding Source: MGI

    GO - Biological processi

    1. Rac protein signal transduction Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epidermal growth factor receptor kinase substrate 8-like protein 1
    Short name:
    EPS8-like protein 1
    Alternative name(s):
    Epidermal growth factor receptor pathway substrate 8-related protein 1
    Short name:
    EPS8-related protein 1
    Gene namesi
    Name:Eps8l1
    Synonyms:Eps8r1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1914675. Eps8l1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. ruffle Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 716716Epidermal growth factor receptor kinase substrate 8-like protein 1PRO_0000239083Add
    BLAST

    Proteomic databases

    PaxDbiQ8R5F8.
    PRIDEiQ8R5F8.

    PTM databases

    PhosphoSiteiQ8R5F8.

    Expressioni

    Tissue specificityi

    Detected in placenta, skin, mammary gland, bone marrow and stomach.1 Publication

    Gene expression databases

    ArrayExpressiQ8R5F8.
    BgeeiQ8R5F8.
    CleanExiMM_EPS8L1.
    GenevestigatoriQ8R5F8.

    Interactioni

    Subunit structurei

    Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000083559.

    Structurei

    Secondary structure

    1
    716
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi32 – 4514
    Beta strandi48 – 503
    Helixi55 – 6713
    Beta strandi74 – 807
    Beta strandi82 – 887
    Turni90 – 923
    Beta strandi95 – 1006
    Helixi101 – 1033
    Beta strandi104 – 1107
    Beta strandi119 – 1246
    Beta strandi133 – 1397
    Turni140 – 1423
    Helixi143 – 15816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CY4X-ray1.94A26-165[»]
    2CY5X-ray1.90A26-165[»]
    ProteinModelPortaliQ8R5F8.
    SMRiQ8R5F8. Positions 30-159, 478-536, 605-687.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8R5F8.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini477 – 53660SH3PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili682 – 71332Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi119 – 1224Poly-Leu
    Compositional biasi554 – 56411Poly-ProAdd
    BLAST

    Sequence similaritiesi

    Belongs to the EPS8 family.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG263108.
    GeneTreeiENSGT00390000003646.
    HOGENOMiHOG000060324.
    HOVERGENiHBG003090.
    InParanoidiQ8R5F8.
    KOiK17277.
    PhylomeDBiQ8R5F8.
    TreeFamiTF313069.

    Family and domain databases

    InterProiIPR013625. PTB.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF08416. PTB. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8R5F8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTTTGPEAA PKPSAKSIYE QRKRYSTVVM ADVSQYHVNH LVTFCLGEED    50
    GVHTVEDASR KLAVMDSQGR VWAQEMLLRV SPSQVTLLDP VSKEELESYP 100
    LDAIVRCDAV MPRGRSRSLL LLVCQEPERA QPDVHFFQGL LLGAELIRED 150
    IQGALQNYRS GRGERRAAAL RATQEELRRG ASPAAETPPL QRRPSVRLVI 200
    NTVEPSAVRG RPQVESIPET EEARKPDQAR TTSSADPTSP DLGPRGPELA 250
    GLQAERDVDI LNHVFDDVES FVSRLQKSAE ATRVLEHRER GRRTRRRAAG 300
    EGLLTLRAKP PTEAEYTDVL QKIKYAFSLL ARLRGNIANP SSPELLHFLF 350
    GPLQMIVNTS GGPEFAKSVR RPHLTLEAVT LLRDNVTPGE NELWTSLGDS 400
    WTCPGVELPP EEGSPYSPEF YNGWEPPATD PQGRPWEDPV EKQLQHEKRR 450
    RQQSAPQVAV NGQQDPELET ESQLEEKARK WVLCNYDFQA RNGSELSVKH 500
    RDVLEVLDDR RKWWKVRDHQ GQEGYVPYNI LTPHPGPQVH RSQSPARHLE 550
    TSTPPPPPAP APAPTQVRPQ WDSCDSLNSL DPSEKEKFSQ MLCVNEELQS 600
    RLAQGRSGPS RVTPGPRAQE PQLSPRSEAS VVRAWLQTKG FSSGTVEALG 650
    VLTGAQLFSL QKEELRAVCP EEGARVYSQV TVQRALLEDR EKVSELEAVM 700
    EKQKKKVEGE TKTEVI 716
    Length:716
    Mass (Da):80,028
    Last modified:June 1, 2002 - v1
    Checksum:iE996E664722E885D
    GO

    Sequence cautioni

    The sequence BC027043 differs from that shown. Reason: Frameshift at position 463.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti464 – 4641Missing in BC027043. (PubMed:15489334)Curated
    Sequence conflicti601 – 6011R → S in BC027043. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY074931 mRNA. Translation: AAL76120.1.
    AK019490 mRNA. Translation: BAB31756.2.
    BC027043 mRNA. No translation available.
    CCDSiCCDS20736.1.
    RefSeqiNP_001277345.1. NM_001290416.1.
    NP_080422.1. NM_026146.4.
    XP_006540387.1. XM_006540324.1.
    XP_006540388.1. XM_006540325.1.
    XP_006540389.1. XM_006540326.1.
    XP_006540390.1. XM_006540327.1.
    UniGeneiMm.360392.

    Genome annotation databases

    EnsembliENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
    ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.
    GeneIDi67425.
    KEGGimmu:67425.
    UCSCiuc009exo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY074931 mRNA. Translation: AAL76120.1 .
    AK019490 mRNA. Translation: BAB31756.2 .
    BC027043 mRNA. No translation available.
    CCDSi CCDS20736.1.
    RefSeqi NP_001277345.1. NM_001290416.1.
    NP_080422.1. NM_026146.4.
    XP_006540387.1. XM_006540324.1.
    XP_006540388.1. XM_006540325.1.
    XP_006540389.1. XM_006540326.1.
    XP_006540390.1. XM_006540327.1.
    UniGenei Mm.360392.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CY4 X-ray 1.94 A 26-165 [» ]
    2CY5 X-ray 1.90 A 26-165 [» ]
    ProteinModelPortali Q8R5F8.
    SMRi Q8R5F8. Positions 30-159, 478-536, 605-687.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000083559.

    PTM databases

    PhosphoSitei Q8R5F8.

    Proteomic databases

    PaxDbi Q8R5F8.
    PRIDEi Q8R5F8.

    Protocols and materials databases

    DNASUi 67425.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000086372 ; ENSMUSP00000083559 ; ENSMUSG00000006154 .
    ENSMUST00000163893 ; ENSMUSP00000125840 ; ENSMUSG00000006154 .
    GeneIDi 67425.
    KEGGi mmu:67425.
    UCSCi uc009exo.1. mouse.

    Organism-specific databases

    CTDi 54869.
    MGIi MGI:1914675. Eps8l1.

    Phylogenomic databases

    eggNOGi NOG263108.
    GeneTreei ENSGT00390000003646.
    HOGENOMi HOG000060324.
    HOVERGENi HBG003090.
    InParanoidi Q8R5F8.
    KOi K17277.
    PhylomeDBi Q8R5F8.
    TreeFami TF313069.

    Miscellaneous databases

    EvolutionaryTracei Q8R5F8.
    NextBioi 324528.
    PROi Q8R5F8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R5F8.
    Bgeei Q8R5F8.
    CleanExi MM_EPS8L1.
    Genevestigatori Q8R5F8.

    Family and domain databases

    InterProi IPR013625. PTB.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF08416. PTB. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure."
      Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C.
      Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Skin.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    4. "The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
      Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
      Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiES8L1_MOUSE
    AccessioniPrimary (citable) accession number: Q8R5F8
    Secondary accession number(s): Q8R0D6, Q9D2M6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3