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Protein

Epidermal growth factor receptor kinase substrate 8-like protein 1

Gene

Eps8l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 1
Short name:
EPS8-like protein 1
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 1
Short name:
EPS8-related protein 1
Gene namesi
Name:Eps8l1
Synonyms:Eps8r1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1914675. Eps8l1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002390831 – 716Epidermal growth factor receptor kinase substrate 8-like protein 1Add BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182PhosphoserineCombined sources1
Modified residuei187PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8R5F8.
PaxDbiQ8R5F8.
PRIDEiQ8R5F8.

PTM databases

iPTMnetiQ8R5F8.
PhosphoSitePlusiQ8R5F8.

Expressioni

Tissue specificityi

Detected in placenta, skin, mammary gland, bone marrow and stomach.1 Publication

Gene expression databases

BgeeiENSMUSG00000006154.
CleanExiMM_EPS8L1.
ExpressionAtlasiQ8R5F8. baseline and differential.
GenevisibleiQ8R5F8. MM.

Interactioni

Subunit structurei

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083559.

Structurei

Secondary structure

1716
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 45Combined sources14
Beta strandi48 – 50Combined sources3
Helixi55 – 67Combined sources13
Beta strandi74 – 80Combined sources7
Beta strandi82 – 88Combined sources7
Turni90 – 92Combined sources3
Beta strandi95 – 100Combined sources6
Helixi101 – 103Combined sources3
Beta strandi104 – 110Combined sources7
Beta strandi119 – 124Combined sources6
Beta strandi133 – 139Combined sources7
Turni140 – 142Combined sources3
Helixi143 – 158Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CY4X-ray1.94A26-165[»]
2CY5X-ray1.90A26-165[»]
ProteinModelPortaliQ8R5F8.
SMRiQ8R5F8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R5F8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini477 – 536SH3PROSITE-ProRule annotationAdd BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili682 – 713Sequence analysisAdd BLAST32

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi119 – 122Poly-Leu4
Compositional biasi554 – 564Poly-ProAdd BLAST11

Sequence similaritiesi

Belongs to the EPS8 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3557. Eukaryota.
ENOG410XT9R. LUCA.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ8R5F8.
KOiK17277.
PhylomeDBiQ8R5F8.
TreeFamiTF313069.

Family and domain databases

CDDicd01210. PTB_EPS8. 1 hit.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR033928. EPS8_PTB.
IPR011993. PH_dom-like.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R5F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTTGPEAA PKPSAKSIYE QRKRYSTVVM ADVSQYHVNH LVTFCLGEED
60 70 80 90 100
GVHTVEDASR KLAVMDSQGR VWAQEMLLRV SPSQVTLLDP VSKEELESYP
110 120 130 140 150
LDAIVRCDAV MPRGRSRSLL LLVCQEPERA QPDVHFFQGL LLGAELIRED
160 170 180 190 200
IQGALQNYRS GRGERRAAAL RATQEELRRG ASPAAETPPL QRRPSVRLVI
210 220 230 240 250
NTVEPSAVRG RPQVESIPET EEARKPDQAR TTSSADPTSP DLGPRGPELA
260 270 280 290 300
GLQAERDVDI LNHVFDDVES FVSRLQKSAE ATRVLEHRER GRRTRRRAAG
310 320 330 340 350
EGLLTLRAKP PTEAEYTDVL QKIKYAFSLL ARLRGNIANP SSPELLHFLF
360 370 380 390 400
GPLQMIVNTS GGPEFAKSVR RPHLTLEAVT LLRDNVTPGE NELWTSLGDS
410 420 430 440 450
WTCPGVELPP EEGSPYSPEF YNGWEPPATD PQGRPWEDPV EKQLQHEKRR
460 470 480 490 500
RQQSAPQVAV NGQQDPELET ESQLEEKARK WVLCNYDFQA RNGSELSVKH
510 520 530 540 550
RDVLEVLDDR RKWWKVRDHQ GQEGYVPYNI LTPHPGPQVH RSQSPARHLE
560 570 580 590 600
TSTPPPPPAP APAPTQVRPQ WDSCDSLNSL DPSEKEKFSQ MLCVNEELQS
610 620 630 640 650
RLAQGRSGPS RVTPGPRAQE PQLSPRSEAS VVRAWLQTKG FSSGTVEALG
660 670 680 690 700
VLTGAQLFSL QKEELRAVCP EEGARVYSQV TVQRALLEDR EKVSELEAVM
710
EKQKKKVEGE TKTEVI
Length:716
Mass (Da):80,028
Last modified:June 1, 2002 - v1
Checksum:iE996E664722E885D
GO

Sequence cautioni

The sequence BC027043 differs from that shown. Reason: Frameshift at position 463.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464Missing in BC027043 (PubMed:15489334).Curated1
Sequence conflicti601R → S in BC027043 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074931 mRNA. Translation: AAL76120.1.
AK019490 mRNA. Translation: BAB31756.2.
BC027043 mRNA. No translation available.
CCDSiCCDS20736.1.
RefSeqiNP_001277345.1. NM_001290416.1.
NP_080422.1. NM_026146.4.
XP_006540387.1. XM_006540324.3.
XP_006540388.1. XM_006540325.3.
XP_006540389.1. XM_006540326.1.
XP_017167747.1. XM_017312258.1.
UniGeneiMm.360392.

Genome annotation databases

EnsembliENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.
GeneIDi67425.
KEGGimmu:67425.
UCSCiuc009exo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074931 mRNA. Translation: AAL76120.1.
AK019490 mRNA. Translation: BAB31756.2.
BC027043 mRNA. No translation available.
CCDSiCCDS20736.1.
RefSeqiNP_001277345.1. NM_001290416.1.
NP_080422.1. NM_026146.4.
XP_006540387.1. XM_006540324.3.
XP_006540388.1. XM_006540325.3.
XP_006540389.1. XM_006540326.1.
XP_017167747.1. XM_017312258.1.
UniGeneiMm.360392.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CY4X-ray1.94A26-165[»]
2CY5X-ray1.90A26-165[»]
ProteinModelPortaliQ8R5F8.
SMRiQ8R5F8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000083559.

PTM databases

iPTMnetiQ8R5F8.
PhosphoSitePlusiQ8R5F8.

Proteomic databases

MaxQBiQ8R5F8.
PaxDbiQ8R5F8.
PRIDEiQ8R5F8.

Protocols and materials databases

DNASUi67425.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.
GeneIDi67425.
KEGGimmu:67425.
UCSCiuc009exo.2. mouse.

Organism-specific databases

CTDi54869.
MGIiMGI:1914675. Eps8l1.

Phylogenomic databases

eggNOGiKOG3557. Eukaryota.
ENOG410XT9R. LUCA.
GeneTreeiENSGT00390000003646.
HOGENOMiHOG000060324.
HOVERGENiHBG003090.
InParanoidiQ8R5F8.
KOiK17277.
PhylomeDBiQ8R5F8.
TreeFamiTF313069.

Miscellaneous databases

EvolutionaryTraceiQ8R5F8.
PROiQ8R5F8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006154.
CleanExiMM_EPS8L1.
ExpressionAtlasiQ8R5F8. baseline and differential.
GenevisibleiQ8R5F8. MM.

Family and domain databases

CDDicd01210. PTB_EPS8. 1 hit.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR033928. EPS8_PTB.
IPR011993. PH_dom-like.
IPR013625. PTB.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiES8L1_MOUSE
AccessioniPrimary (citable) accession number: Q8R5F8
Secondary accession number(s): Q8R0D6, Q9D2M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.