Q8R5F8 (ES8L1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 1
Short name=EPS8-like protein 1

Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 1
Short name=EPS8-related protein 1

Gene names

Name:	Eps8l1
Synonyms:	Eps8r1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.
Subunit structure	Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Detected in placenta, skin, mammary gland, bone marrow and stomach. Ref.4
Sequence similarities	Belongs to the EPS8 family. Contains 1 SH3 domain.
Sequence caution	The sequence BC027043 differs from that shown. Reason: Frameshift at position 463.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil SH3 domain
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rac protein signal transduction Inferred from sequence alignment Ref.4. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell ruffle Inferred from sequence alignment Ref.4. Source: MGI
Molecular_function	actin filament binding Inferred from sequence alignment Ref.4. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 716

716

Epidermal growth factor receptor kinase substrate 8-like protein 1

PRO_0000239083

Regions

Domain

477 – 536

60

SH3

Coiled coil

682 – 713

32

Potential

Compositional bias

119 – 122

4

Poly-Leu

Compositional bias

554 – 564

11

Poly-Pro

Experimental info

Sequence conflict

464

1

Missing in BC027043. Ref.3

Sequence conflict

601

1

R → S in BC027043. Ref.3

Secondary structure

1

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716

Helix Strand Turn

Details...

Beta strand

32 – 45

14

Beta strand

48 – 50

3

Helix

55 – 67

13

Beta strand

74 – 80

7

Beta strand

82 – 88

7

Turn

90 – 92

3

Beta strand

95 – 100

6

Helix

101 – 103

3

Beta strand

104 – 110

7

Beta strand

119 – 124

6

Beta strand

133 – 139

7

Turn

140 – 142

3

Helix

143 – 158

16

Sequences

	Sequence		Length	Mass (Da)
	Q8R5F8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E996E664722E885D	FASTA	716	80,028
	10 20 30 40 50 60 MSTTTGPEAA PKPSAKSIYE QRKRYSTVVM ADVSQYHVNH LVTFCLGEED GVHTVEDASR 70 80 90 100 110 120 KLAVMDSQGR VWAQEMLLRV SPSQVTLLDP VSKEELESYP LDAIVRCDAV MPRGRSRSLL 130 140 150 160 170 180 LLVCQEPERA QPDVHFFQGL LLGAELIRED IQGALQNYRS GRGERRAAAL RATQEELRRG 190 200 210 220 230 240 ASPAAETPPL QRRPSVRLVI NTVEPSAVRG RPQVESIPET EEARKPDQAR TTSSADPTSP 250 260 270 280 290 300 DLGPRGPELA GLQAERDVDI LNHVFDDVES FVSRLQKSAE ATRVLEHRER GRRTRRRAAG 310 320 330 340 350 360 EGLLTLRAKP PTEAEYTDVL QKIKYAFSLL ARLRGNIANP SSPELLHFLF GPLQMIVNTS 370 380 390 400 410 420 GGPEFAKSVR RPHLTLEAVT LLRDNVTPGE NELWTSLGDS WTCPGVELPP EEGSPYSPEF 430 440 450 460 470 480 YNGWEPPATD PQGRPWEDPV EKQLQHEKRR RQQSAPQVAV NGQQDPELET ESQLEEKARK 490 500 510 520 530 540 WVLCNYDFQA RNGSELSVKH RDVLEVLDDR RKWWKVRDHQ GQEGYVPYNI LTPHPGPQVH 550 560 570 580 590 600 RSQSPARHLE TSTPPPPPAP APAPTQVRPQ WDSCDSLNSL DPSEKEKFSQ MLCVNEELQS 610 620 630 640 650 660 RLAQGRSGPS RVTPGPRAQE PQLSPRSEAS VVRAWLQTKG FSSGTVEALG VLTGAQLFSL 670 680 690 700 710 QKEELRAVCP EEGARVYSQV TVQRALLEDR EKVSELEAVM EKQKKKVEGE TKTEVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure." Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C. Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Skin.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
[4]	"The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway." Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G. Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY074931 mRNA. Translation: AAL76120.1.
AK019490 mRNA. Translation: BAB31756.2.
BC027043 mRNA. No translation available.

IPI

IPI00154007.

RefSeq

NP_080422.1. NM_026146.3.

UniGene

Mm.360392.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CY4	X-ray	1.94	A	26-165	[»]
2CY5	X-ray	1.90	A	26-165	[»]

ProteinModelPortal

Q8R5F8.

SMR

Q8R5F8. Positions 30-159, 478-536, 605-687.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000083559.

PTM databases

PhosphoSite

Q8R5F8.

Proteomic databases

PaxDb

Q8R5F8.

PRIDE

Q8R5F8.

Protocols and materials databases

DNASU

67425.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.

GeneID

67425.

KEGG

mmu:67425.

UCSC

uc009exo.1. mouse.

Organism-specific databases

CTD

54869.

MGI

MGI:1914675. Eps8l1.

Phylogenomic databases

eggNOG

NOG263108.

GeneTree

ENSGT00390000003646.

HOGENOM

HOG000060324.

HOVERGEN

HBG003090.

InParanoid

Q8R5F8.

OrthoDB

EOG412M51.

Gene expression databases

ArrayExpress

Q8R5F8.

Bgee

Q8R5F8.

CleanEx

MM_EPS8L1.

Genevestigator

Q8R5F8.

GermOnline

ENSMUSG00000006154. Mus musculus.

Family and domain databases

InterPro

IPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8R5F8.

NextBio

324528.

SOURCE

Search...

Entry information

Entry name

ES8L1_MOUSE

Accession

Primary (citable) accession number: Q8R5F8
Secondary accession number(s): Q8R0D6, Q9D2M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program