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Q8R5F8 (ES8L1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epidermal growth factor receptor kinase substrate 8-like protein 1

Short name=EPS8-like protein 1
Alternative name(s):
Epidermal growth factor receptor pathway substrate 8-related protein 1
Short name=EPS8-related protein 1
Gene names
Name:Eps8l1
Synonyms:Eps8r1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length716 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton By similarity.

Subunit structure

Interacts with ABI1. Part of a complex that contains SOS1, ABI1 and EPS8L2. Associates with F-actin By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Detected in placenta, skin, mammary gland, bone marrow and stomach. Ref.4

Sequence similarities

Belongs to the EPS8 family.

Contains 1 SH3 domain.

Sequence caution

The sequence BC027043 differs from that shown. Reason: Frameshift at position 463.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainCoiled coil
SH3 domain
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRac protein signal transduction

Inferred from sequence alignment Ref.4. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

ruffle

Inferred from sequence alignment Ref.4. Source: MGI

   Molecular_functionactin filament binding

Inferred from sequence alignment Ref.4. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 716716Epidermal growth factor receptor kinase substrate 8-like protein 1
PRO_0000239083

Regions

Domain477 – 53660SH3
Coiled coil682 – 71332 Potential
Compositional bias119 – 1224Poly-Leu
Compositional bias554 – 56411Poly-Pro

Experimental info

Sequence conflict4641Missing in BC027043. Ref.3
Sequence conflict6011R → S in BC027043. Ref.3

Secondary structure

....................... 716
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8R5F8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: E996E664722E885D

FASTA71680,028
        10         20         30         40         50         60 
MSTTTGPEAA PKPSAKSIYE QRKRYSTVVM ADVSQYHVNH LVTFCLGEED GVHTVEDASR 

        70         80         90        100        110        120 
KLAVMDSQGR VWAQEMLLRV SPSQVTLLDP VSKEELESYP LDAIVRCDAV MPRGRSRSLL 

       130        140        150        160        170        180 
LLVCQEPERA QPDVHFFQGL LLGAELIRED IQGALQNYRS GRGERRAAAL RATQEELRRG 

       190        200        210        220        230        240 
ASPAAETPPL QRRPSVRLVI NTVEPSAVRG RPQVESIPET EEARKPDQAR TTSSADPTSP 

       250        260        270        280        290        300 
DLGPRGPELA GLQAERDVDI LNHVFDDVES FVSRLQKSAE ATRVLEHRER GRRTRRRAAG 

       310        320        330        340        350        360 
EGLLTLRAKP PTEAEYTDVL QKIKYAFSLL ARLRGNIANP SSPELLHFLF GPLQMIVNTS 

       370        380        390        400        410        420 
GGPEFAKSVR RPHLTLEAVT LLRDNVTPGE NELWTSLGDS WTCPGVELPP EEGSPYSPEF 

       430        440        450        460        470        480 
YNGWEPPATD PQGRPWEDPV EKQLQHEKRR RQQSAPQVAV NGQQDPELET ESQLEEKARK 

       490        500        510        520        530        540 
WVLCNYDFQA RNGSELSVKH RDVLEVLDDR RKWWKVRDHQ GQEGYVPYNI LTPHPGPQVH 

       550        560        570        580        590        600 
RSQSPARHLE TSTPPPPPAP APAPTQVRPQ WDSCDSLNSL DPSEKEKFSQ MLCVNEELQS 

       610        620        630        640        650        660 
RLAQGRSGPS RVTPGPRAQE PQLSPRSEAS VVRAWLQTKG FSSGTVEALG VLTGAQLFSL 

       670        680        690        700        710 
QKEELRAVCP EEGARVYSQV TVQRALLEDR EKVSELEAVM EKQKKKVEGE TKTEVI 

« Hide

References

« Hide 'large scale' references
[1]"In silico analysis of the EPS8 gene family: genomic organization, expression profile, and protein structure."
Tocchetti A., Confalonieri S., Scita G., Di Fiore P.P., Betsholtz C.
Genomics 81:234-244(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Skin.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Retina.
[4]"The eps8 family of proteins links growth factor stimulation to actin reorganization generating functional redundancy in the Ras/Rac pathway."
Offenhaeuser N., Borgonovo A., Disanza A., Romano P., Ponzanelli I., Iannolo G., Di Fiore P.P., Scita G.
Mol. Biol. Cell 15:91-98(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY074931 mRNA. Translation: AAL76120.1.
AK019490 mRNA. Translation: BAB31756.2.
BC027043 mRNA. No translation available.
CCDSCCDS20736.1.
RefSeqNP_001277345.1. NM_001290416.1.
NP_080422.1. NM_026146.4.
XP_006540387.1. XM_006540324.1.
XP_006540388.1. XM_006540325.1.
XP_006540389.1. XM_006540326.1.
XP_006540390.1. XM_006540327.1.
UniGeneMm.360392.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CY4X-ray1.94A26-165[»]
2CY5X-ray1.90A26-165[»]
ProteinModelPortalQ8R5F8.
SMRQ8R5F8. Positions 30-159, 478-536, 605-687.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000083559.

PTM databases

PhosphoSiteQ8R5F8.

Proteomic databases

PaxDbQ8R5F8.
PRIDEQ8R5F8.

Protocols and materials databases

DNASU67425.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000086372; ENSMUSP00000083559; ENSMUSG00000006154.
ENSMUST00000163893; ENSMUSP00000125840; ENSMUSG00000006154.
GeneID67425.
KEGGmmu:67425.
UCSCuc009exo.1. mouse.

Organism-specific databases

CTD54869.
MGIMGI:1914675. Eps8l1.

Phylogenomic databases

eggNOGNOG263108.
GeneTreeENSGT00390000003646.
HOGENOMHOG000060324.
HOVERGENHBG003090.
InParanoidQ8R5F8.
KOK17277.
PhylomeDBQ8R5F8.
TreeFamTF313069.

Gene expression databases

ArrayExpressQ8R5F8.
BgeeQ8R5F8.
CleanExMM_EPS8L1.
GenevestigatorQ8R5F8.

Family and domain databases

InterProIPR013625. PTB.
IPR001452. SH3_domain.
[Graphical view]
PfamPF08416. PTB. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ8R5F8.
NextBio324528.
PROQ8R5F8.
SOURCESearch...

Entry information

Entry nameES8L1_MOUSE
AccessionPrimary (citable) accession number: Q8R5F8
Secondary accession number(s): Q8R0D6, Q9D2M6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot