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Protein

N-lysine methyltransferase SMYD2

Gene

Smyd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.1 Publication

Miscellaneous

Although specifically expressed in cardiomyocytes, a conditional deletion in heart does not lead to any visible phenotype.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei240Peptide substrate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 90MYND-typePROSITE-ProRule annotationAdd BLAST39

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BRENDAi2.1.1.43 3474
ReactomeiR-MMU-3214841 PKMTs methylate histone lysines
R-MMU-6804760 Regulation of TP53 Activity through Methylation

Names & Taxonomyi

Protein namesi
Recommended name:
N-lysine methyltransferase SMYD2 (EC:2.1.1.-)
Alternative name(s):
Histone methyltransferase SMYD2 (EC:2.1.1.43)
SET and MYND domain-containing protein 2
Gene namesi
Name:Smyd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915889 Smyd2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi240Y → F: Abolishes methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002183101 – 433N-lysine methyltransferase SMYD2Add BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei283PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8R5A0
MaxQBiQ8R5A0
PaxDbiQ8R5A0
PeptideAtlasiQ8R5A0
PRIDEiQ8R5A0

PTM databases

iPTMnetiQ8R5A0
PhosphoSitePlusiQ8R5A0

Expressioni

Tissue specificityi

Highly expressed in heart, skeletal muscle and brain tissue. During cardiac development, it is differentially expressed with highest expression in the neonatal heart while very low expression is detected at E12.5 and adult. Specifically expressed in cardiomyocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000026603
CleanExiMM_SMYD2
GenevisibleiQ8R5A0 MM

Interactioni

Subunit structurei

Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1 (By similarity). Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TP53P046373EBI-15612527,EBI-366083From Homo sapiens.

GO - Molecular functioni

  • p53 binding Source: MGI
  • RNA polymerase II core binding Source: UniProtKB

Protein-protein interaction databases

BioGridi230559, 2 interactors
DIPiDIP-60503N
IntActiQ8R5A0, 1 interactor
STRINGi10090.ENSMUSP00000027897

Structurei

Secondary structure

1433
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Beta strandi19 – 25Combined sources7
Beta strandi32 – 37Combined sources6
Beta strandi39 – 43Combined sources5
Helixi45 – 47Combined sources3
Turni48 – 50Combined sources3
Turni53 – 55Combined sources3
Turni66 – 68Combined sources3
Beta strandi72 – 75Combined sources4
Helixi76 – 86Combined sources11
Turni87 – 89Combined sources3
Helixi90 – 95Combined sources6
Helixi104 – 118Combined sources15
Helixi124 – 126Combined sources3
Helixi131 – 133Combined sources3
Helixi138 – 140Combined sources3
Helixi143 – 160Combined sources18
Turni161 – 163Combined sources3
Helixi169 – 182Combined sources14
Beta strandi184 – 187Combined sources4
Beta strandi193 – 198Combined sources6
Helixi202 – 204Combined sources3
Beta strandi205 – 207Combined sources3
Beta strandi212 – 218Combined sources7
Beta strandi221 – 228Combined sources8
Beta strandi235 – 238Combined sources4
Helixi247 – 258Combined sources12
Helixi265 – 269Combined sources5
Helixi273 – 276Combined sources4
Helixi288 – 308Combined sources21
Turni309 – 311Combined sources3
Helixi314 – 328Combined sources15
Turni329 – 331Combined sources3
Helixi337 – 352Combined sources16
Helixi356 – 373Combined sources18
Helixi379 – 394Combined sources16
Helixi398 – 415Combined sources18
Helixi421 – 431Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QWVX-ray2.03A1-433[»]
3QWWX-ray1.80A1-433[»]
ProteinModelPortaliQ8R5A0
SMRiQ8R5A0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8R5A0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 241SETPROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 19S-adenosyl-L-methionine bindingBy similarity3
Regioni183 – 185Peptide substrate bindingBy similarity3
Regioni206 – 207S-adenosyl-L-methionine bindingBy similarity2
Regioni258 – 260S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri52 – 90MYND-typePROSITE-ProRule annotationAdd BLAST39

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2084 Eukaryota
COG2940 LUCA
GeneTreeiENSGT00530000063077
HOGENOMiHOG000007850
HOVERGENiHBG098536
InParanoidiQ8R5A0
KOiK11426
OMAiLAKQKTH
OrthoDBiEOG091G066T
PhylomeDBiQ8R5A0
TreeFamiTF106487

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR001214 SET_dom
IPR011990 TPR-like_helical_dom_sf
IPR002893 Znf_MYND
PfamiView protein in Pfam
PF00856 SET, 1 hit
PF01753 zf-MYND, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50280 SET, 1 hit
PS01360 ZF_MYND_1, 1 hit
PS50865 ZF_MYND_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R5A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAEARGGLE RFCSAGKGRG LRALRPFHVG DLLFSCPAYA CVLTVGERGH
60 70 80 90 100
HCECCFARKE GLSKCGRCKQ AFYCDVECQK EDWPLHKLEC SSMVVLGENW
110 120 130 140 150
NPSETVRLTA RILAKQKIHP ERTPSEKLLA VREFESHLDK LDNEKKDLIQ
160 170 180 190 200
SDIAALHQFY SKYLEFPDHS SLVVLFAQVN CNGFTIEDEE LSHLGSAIFP
210 220 230 240 250
DVALMNHSCC PNVIVTYKGT LAEVRAVQEI HPGDEVFTSY IDLLYPTEDR
260 270 280 290 300
NDRLRDSYFF TCECRECTTK DKDKAKVEVR KLSSPPQAEA IRDMVRYARN
310 320 330 340 350
VIEEFRRAKH YKSPSELLEI CELSQEKMSS VFEDSNVYML HMMYQAMGVC
360 370 380 390 400
LYMQDWEGAL KYGQKIIKPY SKHYPVYSLN VASMWLKLGR LYMGLENKAA
410 420 430
GEKALKKAIA IMEVAHGKDH PYISEIKQEI ESH
Length:433
Mass (Da):49,567
Last modified:June 1, 2002 - v1
Checksum:i5978E9A3FC2CDCE4
GO

Sequence cautioni

The sequence BAE29912 differs from that shown. Deletion within an exon that does not correspond to an intron.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150857 mRNA Translation: BAE29912.1 Sequence problems.
BC023119 mRNA Translation: AAH23119.1
CCDSiCCDS35821.1
RefSeqiNP_081072.1, NM_026796.1
UniGeneiMm.156895

Genome annotation databases

EnsembliENSMUST00000027897; ENSMUSP00000027897; ENSMUSG00000026603
GeneIDi226830
KEGGimmu:226830
UCSCiuc007eax.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSMYD2_MOUSE
AccessioniPrimary (citable) accession number: Q8R5A0
Secondary accession number(s): Q3UBQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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