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Protein

E3 ubiquitin-protein ligase MIB2

Gene

Mib2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri85 – 132ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri850 – 885RING-type 1PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri929 – 962RING-type 2PROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Notch signaling pathway, Ubl conjugation pathway

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MIB2 (EC:6.3.2.-)
Alternative name(s):
Dystrophin-like protein
Short name:
Dyslike
Mind bomb homolog 2
Short name:
Mind bomb-2
Skeletrophin
Gene namesi
Name:Mib2
Synonyms:Skd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2679684. Mib2.

Subcellular locationi

GO - Cellular componenti

  • early endosome Source: MGI
  • ubiquitin ligase complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi874C → S: No effect. 1 Publication1
Mutagenesisi951C → S: Abolishes ubiquitin ligase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559481 – 973E3 ubiquitin-protein ligase MIB2Add BLAST973

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. Possibly via autoubiquitination.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ8R516.

PTM databases

iPTMnetiQ8R516.
PhosphoSitePlusiQ8R516.

Expressioni

Tissue specificityi

Highly expressed in brain, heart, liver and kidney.1 Publication

Developmental stagei

Highly expressed in neonate and adult, but only slightly in embryos. In E10.5 embryos, it is weakly expressed in the tail bud and limb buds. Expressed in the same pattern than MIB1 in the skin and intestine at postnatal day 1 (P1) and in the hair follicle in the skin in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000029060.
CleanExiMM_MIB2.
ExpressionAtlasiQ8R516. baseline and differential.
GenevisibleiQ8R516. MM.

Interactioni

Subunit structurei

Interacts with actin monomer.By similarity

Protein-protein interaction databases

BioGridi218188. 7 interactors.
IntActiQ8R516. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliQ8R516.
SMRiQ8R516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 80MIB/HERC2 1PROSITE-ProRule annotationAdd BLAST80
Domaini149 – 227MIB/HERC2 2PROSITE-ProRule annotationAdd BLAST79
Repeati480 – 509ANK 1Add BLAST30
Repeati513 – 542ANK 2Add BLAST30
Repeati546 – 575ANK 3Add BLAST30
Repeati579 – 611ANK 4Add BLAST33
Repeati615 – 644ANK 5Add BLAST30
Repeati649 – 679ANK 6Add BLAST31
Repeati683 – 712ANK 7Add BLAST30
Repeati716 – 744ANK 8Add BLAST29
Repeati785 – 814ANK 9Add BLAST30

Sequence similaritiesi

Contains 9 ANK repeats.PROSITE-ProRule annotation
Contains 2 MIB/HERC2 domains.PROSITE-ProRule annotation
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation
Contains 1 ZZ-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri85 – 132ZZ-typePROSITE-ProRule annotationAdd BLAST48
Zinc fingeri850 – 885RING-type 1PROSITE-ProRule annotationAdd BLAST36
Zinc fingeri929 – 962RING-type 2PROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00790000122981.
HOGENOMiHOG000231158.
HOVERGENiHBG068386.
InParanoidiQ8R516.
KOiK10645.
OMAiATCGGHY.
OrthoDBiEOG091G00Z0.
PhylomeDBiQ8R516.
TreeFamiTF324147.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010606. Mib_Herc2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF06701. MIB_HERC2. 2 hits.
PF00569. ZZ. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00184. RING. 2 hits.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS51416. MIB_HERC2. 2 hits.
PS50089. ZF_RING_2. 2 hits.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8R516-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLDPHAGVQ VGMRVVRGMD WKWGQQDGGE GGVGTVVELG RHGSPSTPDR
60 70 80 90 100
TVVVQWDQGT RTNYRAGYQG AHDLLLYDNA QIGIRHPNII CDCCKKHGLR
110 120 130 140 150
GMRWKCRVCF DYDLCTQCYM HNKHDLTHAF ERYETSHSRP VTLSPRQGLP
160 170 180 190 200
RIPLRGIFQG AKVVRGPDWE WGSQDGGEGK TGRVVDIRGW DVETGRSVAS
210 220 230 240 250
VTWADGTTNV YRVGHKGKVD LRCVGEAAGG FYYKEHLPKL GKPAELQRRV
260 270 280 290 300
SADGQPFQRG DKVKCLLDTD VLRDMQEGHG GWNPRMAEFI GQMGTVHRIT
310 320 330 340 350
DRGDVRVQFN HETRWTFHPG ALTKHNSFWV GDVVRVIGDL DTVKRLQAGH
360 370 380 390 400
GEWTDDMAPA LGRVGKVVKV FGDGNLRVAV GGQRWTFSPS CLVAYRPEED
410 420 430 440 450
ANLDVAERAR ENKSAASVSV AGSLSVALDK LRTQKSDPEH PGRLVVEAAL
460 470 480 490 500
GNVARALDLL RRHPEQASYH PALVVDTKNQ GRTALQVAAY LGQVELVRLL
510 520 530 540 550
LQARASMDLP DDEGNTVLHY TAMGNQPEAT RVLLSAGCAV DARNGTRSTA
560 570 580 590 600
LHVAVQRGFL EVVKILCERG CDVNLPDAHA DTPLHSAISA GAGASSIVEV
610 620 630 640 650
LTEVPGIDVT ATNSQGFTLL HHASLKGHVL AVRKILARAR QLVDAKKEDG
660 670 680 690 700
FTALHLAALN NHREVAQVLI REGRCDVNVR NRKLQSPLHL AVQQAHLGLV
710 720 730 740 750
PLLVDAGCSV NTEDEEGDTA LHVALQRHQL LPLVADRAGG DPGPLQLLSR
760 770 780 790 800
LQASGLPGCT ELTVGAAVAC FLALEGADVS YANHRGRSPL DLATEGRVLK
810 820 830 840 850
ALQGCAQRFR ERQAGGGGGV PPGPRHVLST PNTVTNLHVS GTAGPEAAEC
860 870 880 890 900
LVCSELALLI LFSPCQHRTV CEECARRMKK CIRCQVVISK KLRPDGSEVV
910 920 930 940 950
NAIQVPGPPR QLVEELQSRY RQMEERITCP ICIDSHIRLV FQCGHGACAP
960 970
CGAALNACPI CRQPIRDRIQ IFV
Length:973
Mass (Da):105,961
Last modified:July 5, 2005 - v2
Checksum:i3E9406DEB30541D7
GO
Isoform 2 (identifier: Q8R516-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     289-324: Missing.
     415-422: Missing.
     467-474: Missing.

Show »
Length:921
Mass (Da):100,287
Checksum:i5D2B2CBFBFA9EA69
GO
Isoform 3 (identifier: Q8R516-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     289-294: FIGQMG → VSHLFC
     295-973: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:294
Mass (Da):32,911
Checksum:i8D99C17F39E05DB0
GO

Sequence cautioni

The sequence BAB79447 differs from that shown. Reason: Frameshift at positions 151 and 161.Curated
The sequence BAB86856 differs from that shown. Reason: Frameshift at positions 780 and 840.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89I → T in BAB79447 (Ref. 2) Curated1
Sequence conflicti89I → T in BAB86856 (Ref. 2) Curated1
Sequence conflicti131E → G in BAB79447 (Ref. 2) Curated1
Sequence conflicti131E → G in BAB86856 (Ref. 2) Curated1
Sequence conflicti138S → L in BAB79447 (Ref. 2) Curated1
Sequence conflicti138S → L in BAB86856 (Ref. 2) Curated1
Sequence conflicti393V → S in BAB86856 (Ref. 2) Curated1
Sequence conflicti407E → D in BAB86856 (Ref. 2) Curated1
Sequence conflicti465E → D in BAB86856 (Ref. 2) Curated1
Sequence conflicti487V → L in BAB86856 (Ref. 2) Curated1
Sequence conflicti537G → R in BAB86856 (Ref. 2) Curated1
Sequence conflicti626K → R in BAB86856 (Ref. 2) Curated1
Sequence conflicti745 – 750LQLLSR → CSCCQG in BAC25227 (PubMed:16141072).Curated6
Sequence conflicti904Q → R in BAB86856 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014397289 – 324Missing in isoform 2. 1 PublicationAdd BLAST36
Alternative sequenceiVSP_014398289 – 294FIGQMG → VSHLFC in isoform 3. 1 Publication6
Alternative sequenceiVSP_014399295 – 973Missing in isoform 3. 1 PublicationAdd BLAST679
Alternative sequenceiVSP_014400415 – 422Missing in isoform 2. 1 Publication8
Alternative sequenceiVSP_014401467 – 474Missing in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY974090 mRNA. Translation: AAX84652.1.
AB063290 mRNA. Translation: BAB79447.1. Frameshift.
AB072336 mRNA. Translation: BAB86856.1. Frameshift.
AL627405 Genomic DNA. Translation: CAM16749.1.
CH466594 Genomic DNA. Translation: EDL15023.1.
BC058086 mRNA. No translation available.
AK008671 mRNA. Translation: BAC25227.1.
CCDSiCCDS19035.1. [Q8R516-2]
RefSeqiNP_001243037.2. NM_001256108.2. [Q8R516-2]
NP_660106.2. NM_145124.3. [Q8R516-2]
UniGeneiMm.235738.
Mm.490124.

Genome annotation databases

EnsembliENSMUST00000103176; ENSMUSP00000099465; ENSMUSG00000029060. [Q8R516-2]
GeneIDi76580.
KEGGimmu:76580.
UCSCiuc008wee.2. mouse. [Q8R516-1]
uc008wef.2. mouse. [Q8R516-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY974090 mRNA. Translation: AAX84652.1.
AB063290 mRNA. Translation: BAB79447.1. Frameshift.
AB072336 mRNA. Translation: BAB86856.1. Frameshift.
AL627405 Genomic DNA. Translation: CAM16749.1.
CH466594 Genomic DNA. Translation: EDL15023.1.
BC058086 mRNA. No translation available.
AK008671 mRNA. Translation: BAC25227.1.
CCDSiCCDS19035.1. [Q8R516-2]
RefSeqiNP_001243037.2. NM_001256108.2. [Q8R516-2]
NP_660106.2. NM_145124.3. [Q8R516-2]
UniGeneiMm.235738.
Mm.490124.

3D structure databases

ProteinModelPortaliQ8R516.
SMRiQ8R516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218188. 7 interactors.
IntActiQ8R516. 1 interactor.

PTM databases

iPTMnetiQ8R516.
PhosphoSitePlusiQ8R516.

Proteomic databases

PRIDEiQ8R516.

Protocols and materials databases

DNASUi76580.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103176; ENSMUSP00000099465; ENSMUSG00000029060. [Q8R516-2]
GeneIDi76580.
KEGGimmu:76580.
UCSCiuc008wee.2. mouse. [Q8R516-1]
uc008wef.2. mouse. [Q8R516-2]

Organism-specific databases

CTDi142678.
MGIiMGI:2679684. Mib2.

Phylogenomic databases

GeneTreeiENSGT00790000122981.
HOGENOMiHOG000231158.
HOVERGENiHBG068386.
InParanoidiQ8R516.
KOiK10645.
OMAiATCGGHY.
OrthoDBiEOG091G00Z0.
PhylomeDBiQ8R516.
TreeFamiTF324147.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiMib2. mouse.
PROiQ8R516.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029060.
CleanExiMM_MIB2.
ExpressionAtlasiQ8R516. baseline and differential.
GenevisibleiQ8R516. MM.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR010606. Mib_Herc2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF06701. MIB_HERC2. 2 hits.
PF00569. ZZ. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00184. RING. 2 hits.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS51416. MIB_HERC2. 2 hits.
PS50089. ZF_RING_2. 2 hits.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIB2_MOUSE
AccessioniPrimary (citable) accession number: Q8R516
Secondary accession number(s): A2A9P9
, Q52QU8, Q6PEF6, Q8C1N7, Q8VIB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.