Q8R4Z1 (SPA12_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serpin A12

Alternative name(s):
Visceral adipose tissue-derived serine protease inhibitor
Short name=Vaspin
Visceral adipose-specific serpin

Gene names

Name:

Serpina12

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Ref.1
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in visceral adipose tissues. Ref.1
Developmental stage	Barely detectable in at 6 weeks and is highly expressed in adipocytes of visceral white adipose tissues at 30 weeks. Ref.1
Sequence similarities	Belongs to the serpin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glucose metabolic process Inferred from expression pattern Ref.1. Source: RGD regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 411	391	Serpin A12		PRO_0000041978
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	267	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8R4Z1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 29FA271FF8CC8A2D	FASTA	411	47,527
	10 20 30 40 50 60 MNLVLGLGLF LAGLLTVKGL LQDRDAPDTY ESPVRVQEWR GKKDARELTR HNMEFGFKLL 70 80 90 100 110 120 QRLASNSRQG NIFLSPLSIS TAFSMLSLGA QNSTLEEIRE GFNFKEMSDR DMHMGFHYLL 130 140 150 160 170 180 QKLNRETQDV KMSIGNALFM DQRLRPQQRF LKLAKNLYDA DMILTNFQDL ENTQKNINKY 190 200 210 220 230 240 ISRKTHNRIE NMVKNIDPGT VMLLTNYIYF QGRWQYEFDP KQTKEEDFFI EEGKTVKVPM 250 260 270 280 290 300 MFQRGMYDMA YDSQLSCTIL EMPYRGNITA TFVLPDSGKL RLLEQGLQAD IFAKWKSLLS 310 320 330 340 350 360 KRVVDVWVPR LHISATYNMK KVLSRLGISK IFEEHGDLTR ISSHRSLKVG EAVHKAELRM 370 380 390 400 410 NEKGTEGAAG SGAQTLPMET PRRMKLNAPF LMMIYENLMP SMIFLARIYN P

References

[1]

"Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity." Hida K., Wada J., Eguchi J., Zhang H., Baba M., Seida A., Hashimoto I., Okada T., Yasuhara A., Nakatsuka A., Shikata K., Hourai S., Futami J., Watanabe E., Matsuki Y., Hiramatsu R., Akagi S., Makino H., Kanwar Y.S. Proc. Natl. Acad. Sci. U.S.A. 102:10610-10615(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: OLETF. Tissue: Adipose tissue.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF245398 mRNA. Translation: AAL99574.1.
IPI	IPI00778759.
UniGene	Rn.79035.
3D structure databases
ProteinModelPortal	Q8R4Z1.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000012960.
Protein family/group databases
MEROPS	I04.965.
PTM databases
PhosphoSite	Q8R4Z1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:708485. rat.
Organism-specific databases
RGD	708485. Serpina12.
Phylogenomic databases
eggNOG	COG4826.
HOGENOM	HOG000238521.
HOVERGEN	HBG005957.
OrthoDB	EOG4CG088.
Gene expression databases
Genevestigator	Q8R4Z1.
GermOnline	ENSRNOG00000011141. Rattus norvegicus.
Family and domain databases
InterPro	IPR000295. Prot_inh_Lserp2. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
PRINTS	PR00780. LEUSERPINII.
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPA12_RAT

Accession

Primary (citable) accession number: Q8R4Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program