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Protein

Alkaline ceramidase 1

Gene

Acer1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids.1 Publication

Catalytic activityi

N-acylsphingosine + H2O = a carboxylate + sphingosine.

Enzyme regulationi

Inhibited by sphingosine. Inhibited by Mn2+, Zn2+, and Cu2+ in a dose-dependent manner. Slightly activated by Ca2+ in a dose-dependent manner.1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

GO - Molecular functioni

  • ceramidase activity Source: MGI
  • dihydroceramidase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism

Enzyme and pathway databases

BRENDAi3.5.1.23. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Chemistry databases

SwissLipidsiSLP:000000676.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline ceramidase 1 (EC:3.5.1.23)
Short name:
AlkCDase 1
Short name:
Alkaline CDase 1
Short name:
maCER1
Alternative name(s):
Acylsphingosine deacylase 3
N-acylsphingosine amidohydrolase 3
Gene namesi
Name:Acer1
Synonyms:Asah3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2181962. Acer1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36LumenalSequence analysisAdd BLAST36
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Topological domaini58 – 72CytoplasmicSequence analysisAdd BLAST15
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 126CytoplasmicSequence analysisAdd BLAST12
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 149LumenalSequence analysis2
Transmembranei150 – 167HelicalSequence analysisAdd BLAST18
Topological domaini168 – 177CytoplasmicSequence analysis10
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 215LumenalSequence analysisAdd BLAST17
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 273CytoplasmicSequence analysisAdd BLAST37

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002477461 – 273Alkaline ceramidase 1Add BLAST273

Proteomic databases

PaxDbiQ8R4X1.
PRIDEiQ8R4X1.

Expressioni

Tissue specificityi

Highly expressed in skin. Weakly or not expressed in other tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000045019.
GenevisibleiQ8R4X1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062037.

Structurei

3D structure databases

ProteinModelPortaliQ8R4X1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline ceramidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000110920.
HOGENOMiHOG000220878.
InParanoidiQ8R4X1.
KOiK01441.
OMAiVLAISCW.
OrthoDBiEOG091G0HT1.
PhylomeDBiQ8R4X1.
TreeFamiTF313019.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R4X1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVPGTRAKM SSIFAYQSSE VDWCESNFQH SELVAEFYNT FSNVFFLIFG
60 70 80 90 100
PLMMFLMHPY AQKRTRCFYG VSVLFMLIGL FSMYFHMTLS FLGQLLDEIS
110 120 130 140 150
ILWLLASGYS VWLPRCYFPK FVKGNRFYFS CLVTITTIIS TFLTFVKPTV
160 170 180 190 200
NAYALNSIAI HILYIVRTEY KKIRDDDLRH LIAVSVVLWA AALTSWISDR
210 220 230 240 250
VLCSFWQRIH FYYLHSIWHV LISITFPYGI VTMALVDAKY EMPDKTLKVH
260 270
YWPRDSWVIG LPYVEIQEND KNC
Length:273
Mass (Da):32,082
Last modified:June 1, 2002 - v1
Checksum:i0B84D015D7870219
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347023 mRNA. Translation: AAL83821.1.
AK028901 mRNA. Translation: BAC26186.1.
AK075884 mRNA. Translation: BAC36029.1.
BC130254 mRNA. Translation: AAI30255.1.
CCDSiCCDS28919.1.
RefSeqiNP_783858.1. NM_175731.4.
UniGeneiMm.218784.

Genome annotation databases

EnsembliENSMUST00000056113; ENSMUSP00000062037; ENSMUSG00000045019.
GeneIDi171168.
KEGGimmu:171168.
UCSCiuc008ddl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347023 mRNA. Translation: AAL83821.1.
AK028901 mRNA. Translation: BAC26186.1.
AK075884 mRNA. Translation: BAC36029.1.
BC130254 mRNA. Translation: AAI30255.1.
CCDSiCCDS28919.1.
RefSeqiNP_783858.1. NM_175731.4.
UniGeneiMm.218784.

3D structure databases

ProteinModelPortaliQ8R4X1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062037.

Chemistry databases

SwissLipidsiSLP:000000676.

Proteomic databases

PaxDbiQ8R4X1.
PRIDEiQ8R4X1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056113; ENSMUSP00000062037; ENSMUSG00000045019.
GeneIDi171168.
KEGGimmu:171168.
UCSCiuc008ddl.1. mouse.

Organism-specific databases

CTDi125981.
MGIiMGI:2181962. Acer1.

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000110920.
HOGENOMiHOG000220878.
InParanoidiQ8R4X1.
KOiK01441.
OMAiVLAISCW.
OrthoDBiEOG091G0HT1.
PhylomeDBiQ8R4X1.
TreeFamiTF313019.

Enzyme and pathway databases

BRENDAi3.5.1.23. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROiQ8R4X1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045019.
GenevisibleiQ8R4X1. MM.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACER1_MOUSE
AccessioniPrimary (citable) accession number: Q8R4X1
Secondary accession number(s): A1L3S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.