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Protein

Alkaline ceramidase 1

Gene

Acer1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo-phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids.1 Publication

Catalytic activityi

N-acylsphingosine + H2O = a carboxylate + sphingosine.

Enzyme regulationi

Inhibited by sphingosine. Inhibited by Mn2+, Zn2+, and Cu2+ in a dose-dependent manner. Slightly activated by Ca2+ in a dose-dependent manner.1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism

Enzyme and pathway databases

BRENDAi3.5.1.23 3474
ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis

Chemistry databases

SwissLipidsiSLP:000000676

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline ceramidase 1 (EC:3.5.1.23)
Short name:
AlkCDase 1
Short name:
Alkaline CDase 1
Short name:
maCER1
Alternative name(s):
Acylsphingosine deacylase 3
N-acylsphingosine amidohydrolase 3
Gene namesi
Name:Acer1
Synonyms:Asah3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2181962 Acer1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36LumenalSequence analysisAdd BLAST36
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Topological domaini58 – 72CytoplasmicSequence analysisAdd BLAST15
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 126CytoplasmicSequence analysisAdd BLAST12
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 149LumenalSequence analysis2
Transmembranei150 – 167HelicalSequence analysisAdd BLAST18
Topological domaini168 – 177CytoplasmicSequence analysis10
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 215LumenalSequence analysisAdd BLAST17
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 273CytoplasmicSequence analysisAdd BLAST37

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002477461 – 273Alkaline ceramidase 1Add BLAST273

Proteomic databases

PaxDbiQ8R4X1
PRIDEiQ8R4X1

Expressioni

Tissue specificityi

Highly expressed in skin. Weakly or not expressed in other tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000045019
GenevisibleiQ8R4X1 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062037

Structurei

3D structure databases

ProteinModelPortaliQ8R4X1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline ceramidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2329 Eukaryota
ENOG4111RPN LUCA
GeneTreeiENSGT00730000110920
HOGENOMiHOG000220878
InParanoidiQ8R4X1
KOiK01441
OMAiSGYSIWM
OrthoDBiEOG091G0HT1
PhylomeDBiQ8R4X1
TreeFamiTF313019

Family and domain databases

InterProiView protein in InterPro
IPR008901 Ceramidase
PfamiView protein in Pfam
PF05875 Ceramidase, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R4X1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVPGTRAKM SSIFAYQSSE VDWCESNFQH SELVAEFYNT FSNVFFLIFG
60 70 80 90 100
PLMMFLMHPY AQKRTRCFYG VSVLFMLIGL FSMYFHMTLS FLGQLLDEIS
110 120 130 140 150
ILWLLASGYS VWLPRCYFPK FVKGNRFYFS CLVTITTIIS TFLTFVKPTV
160 170 180 190 200
NAYALNSIAI HILYIVRTEY KKIRDDDLRH LIAVSVVLWA AALTSWISDR
210 220 230 240 250
VLCSFWQRIH FYYLHSIWHV LISITFPYGI VTMALVDAKY EMPDKTLKVH
260 270
YWPRDSWVIG LPYVEIQEND KNC
Length:273
Mass (Da):32,082
Last modified:June 1, 2002 - v1
Checksum:i0B84D015D7870219
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF347023 mRNA Translation: AAL83821.1
AK028901 mRNA Translation: BAC26186.1
AK075884 mRNA Translation: BAC36029.1
BC130254 mRNA Translation: AAI30255.1
CCDSiCCDS28919.1
RefSeqiNP_783858.1, NM_175731.4
UniGeneiMm.218784

Genome annotation databases

EnsembliENSMUST00000056113; ENSMUSP00000062037; ENSMUSG00000045019
GeneIDi171168
KEGGimmu:171168
UCSCiuc008ddl.1 mouse

Similar proteinsi

Entry informationi

Entry nameiACER1_MOUSE
AccessioniPrimary (citable) accession number: Q8R4X1
Secondary accession number(s): A1L3S2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome