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Protein

Carboxypeptidase Z

Gene

Cpz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves substrates with C-terminal arginine residues. Probably modulates the Wnt signaling pathway, by cleaving some undefined protein. May play a role in cleavage during prohormone processing (By similarity).By similarity

Cofactori

Zn2+By similarity

Enzyme regulationi

Inhibited by 2-mercaptomethyl-3-guanidinoethylthiopropanoic acid (MGTA) and guanidinoethylmercaptosuccinic acid (GEMSA). Inhibited by chelating agents such as EDTA and EGTA (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi253 – 2531Zinc; catalyticBy similarity
Metal bindingi256 – 2561Zinc; catalyticBy similarity
Metal bindingi385 – 3851Zinc; catalyticBy similarity
Active sitei477 – 4771Proton donor/acceptorBy similarity

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: MGI
  • serine-type carboxypeptidase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

  • peptide metabolic process Source: GO_Central
  • protein processing Source: MGI
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM14.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase Z (EC:3.4.17.-)
Short name:
CPZ
Gene namesi
Name:Cpz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:88487. Cpz.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: GO_Central
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 654634Carboxypeptidase ZPRO_0000252457Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 114PROSITE-ProRule annotation
Disulfide bondi56 ↔ 107PROSITE-ProRule annotation
Glycosylationi62 – 621N-linked (GlcNAc...)Sequence analysis
Disulfide bondi98 ↔ 134PROSITE-ProRule annotation
Disulfide bondi123 ↔ 162PROSITE-ProRule annotation
Disulfide bondi127 ↔ 151PROSITE-ProRule annotation
Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8R4V4.
PaxDbiQ8R4V4.
PRIDEiQ8R4V4.

Expressioni

Gene expression databases

CleanExiMM_CPZ.
ExpressionAtlasiQ8R4V4. baseline and differential.
GenevisibleiQ8R4V4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039804.

Structurei

3D structure databases

ProteinModelPortaliQ8R4V4.
SMRiQ8R4V4. Positions 48-160, 188-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 165123FZPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated
Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ8R4V4.
KOiK13022.
OMAiIEFSSRP.
OrthoDBiEOG7B8S32.
TreeFamiTF315592.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR020067. Frizzled_dom.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF01392. Fz. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00063. FRI. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS00133. CARBOXYPEPT_ZN_2. 1 hit.
PS50038. FZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTMPLLLAA LAALAVLALA ARPSPSCSPG PDPSGKCQRL VYTHSATCVD
60 70 80 90 100
LHLRTCADAA YNHTSFPTPL EHRSWEAVES SPEYMLLGVI HFLLEGQCNP
110 120 130 140 150
DLRLLGCSVL APRCEGGHTQ RPCRHVCEGL REACQPAFDA IDMAWPYFLD
160 170 180 190 200
CAQYFAPEEE GCYDPLEELR GELDVEEALP SGLPPTFIRF AHHSYAQMVR
210 220 230 240 250
VLKRTAARCS QVAKTYSIGR SFEGKDLVVI EFSSRPGQHE LMEPEVKLIG
260 270 280 290 300
NIHGNEVAGR EVLIYLAQYL CSEYLLGNPR IQRLLNTTRI HLLPSMNPDG
310 320 330 340 350
YEVAAAEGAG YNGWTSGRQN AQNLDLNRNF PDLTSEYYRL ASTRGVRTDH
360 370 380 390 400
IPISQYYWWG KVAPETKAIM KWIQTIPFVL SASLHGGDLV VSYPFDFSKN
410 420 430 440 450
PHEKKMFSPT PDEKMFKLLA RAYADVHPMM MDRSENRCGG NFLKRGSIIN
460 470 480 490 500
GADWYSFTGG MSDFNYLHTN CFEITVELGC VKFPPEEALY GLWQQNKEPL
510 520 530 540 550
LNFLEMVHRG IKGMVTDKYG KPVKNARILV KGIRHDVTTA PDGDYWRLLP
560 570 580 590 600
PGSHIVIAQA PGYSKVMKRV TIPLRMKKAG RVDFILHPLA TGPKNFLPGP
610 620 630 640 650
SRALPRFQDP QREPTQMDFE PPRARRQPAS GSKPWWWAYF TSLSPYKPRW

LLKY
Length:654
Mass (Da):73,721
Last modified:October 3, 2012 - v2
Checksum:iF437569D31BE47C4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti180 – 1801P → A in AAM00219 (PubMed:12944424).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356844 mRNA. Translation: AAM00219.1.
AC116705 Genomic DNA. No translation available.
CH466524 Genomic DNA. Translation: EDL37485.1.
CCDSiCCDS19229.1.
RefSeqiNP_694747.2. NM_153107.2.
UniGeneiMm.108557.

Genome annotation databases

EnsembliENSMUST00000038676; ENSMUSP00000039804; ENSMUSG00000036596.
GeneIDi242939.
KEGGimmu:242939.
UCSCiuc008xdt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356844 mRNA. Translation: AAM00219.1.
AC116705 Genomic DNA. No translation available.
CH466524 Genomic DNA. Translation: EDL37485.1.
CCDSiCCDS19229.1.
RefSeqiNP_694747.2. NM_153107.2.
UniGeneiMm.108557.

3D structure databases

ProteinModelPortaliQ8R4V4.
SMRiQ8R4V4. Positions 48-160, 188-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039804.

Protein family/group databases

MEROPSiM14.012.

Proteomic databases

MaxQBiQ8R4V4.
PaxDbiQ8R4V4.
PRIDEiQ8R4V4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038676; ENSMUSP00000039804; ENSMUSG00000036596.
GeneIDi242939.
KEGGimmu:242939.
UCSCiuc008xdt.1. mouse.

Organism-specific databases

CTDi8532.
MGIiMGI:88487. Cpz.

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ8R4V4.
KOiK13022.
OMAiIEFSSRP.
OrthoDBiEOG7B8S32.
TreeFamiTF315592.

Miscellaneous databases

PROiQ8R4V4.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CPZ.
ExpressionAtlasiQ8R4V4. baseline and differential.
GenevisibleiQ8R4V4. MM.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR020067. Frizzled_dom.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF01392. Fz. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00063. FRI. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS00133. CARBOXYPEPT_ZN_2. 1 hit.
PS50038. FZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Carboxypeptidase Z (CPZ) modulates Wnt signaling and regulates the development of skeletal elements in the chicken."
    Moeller C., Swindell E.C., Kispert A., Eichele G.
    Development 130:5103-5111(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NMRI.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiCBPZ_MOUSE
AccessioniPrimary (citable) accession number: Q8R4V4
Secondary accession number(s): G3X945
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 3, 2012
Last modified: July 6, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.