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Protein

Serine/threonine-protein kinase Sgk2

Gene

Sgk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up-regulates Na+ channels: SCNN1A/ENAC, K+ channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Two specific sites, one in the kinase domain (Thr-193) and the other in the C-terminal regulatory region (Ser-356), need to be phosphorylated for its full activation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64ATPPROSITE-ProRule annotation1
Active sitei159Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 49ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Sgk2 (EC:2.7.11.1)
Alternative name(s):
Serum/glucocorticoid-regulated kinase 2
Gene namesi
Name:Sgk2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620232. Sgk2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866481 – 367Serine/threonine-protein kinase Sgk2Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei193Phosphothreonine; by PDPK1By similarity1
Modified residuei356PhosphoserineSequence analysis1

Post-translational modificationi

Activated by phosphorylation on Ser-356 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-193.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8R4U9.
PRIDEiQ8R4U9.

PTM databases

iPTMnetiQ8R4U9.
PhosphoSitePlusiQ8R4U9.

Expressioni

Tissue specificityi

Expressed in the proximal tubule and thick ascending limb of the loop of Henle (TALH).1 Publication

Gene expression databases

BgeeiENSRNOG00000033573.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040718.

Structurei

3D structure databases

ProteinModelPortaliQ8R4U9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 292Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini293 – 367AGC-kinase C-terminalAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 77Nuclear localization signalBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi343 – 346Poly-Ser4
Compositional biasi360 – 363Poly-Asp4

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ8R4U9.
KOiK13303.
PhylomeDBiQ8R4U9.
TreeFamiTF320906.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8R4U9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSPVGVPS PQPSRANGNI NLGPSANPNA RPTDFDFLKV IGKGNYGKVL
60 70 80 90 100
LAKRKSDGAF YAVKVLQKKS ILKNKEQSHI MAERNVLLKN VRHPFLVGLR
110 120 130 140 150
YSFQTPEKLY FVLDYVNGGE LFFHLQREHR FLEPRARFYT AEVASAIGYL
160 170 180 190 200
HSLNIIYRDL KPENILLDCQ GHVVLTDFGL CKECVEPEET TSTFCGTPEY
210 220 230 240 250
LAPEVLRKEP YDRAVDWWCL GAVLYEMLHG LPPFFNTDVA QMYENILHQP
260 270 280 290 300
LQIPGGRTVA ACDLLQGLLH KDQRQRLGSK EDFLDIKNHM FFSPINWDDL
310 320 330 340 350
YHKRLTPPFN PNVEGPADLK HFDPEFTQEA VSKSIGCTPD TMSSSSGASS
360
AFLGFSYAQD DDDILDS
Length:367
Mass (Da):41,361
Last modified:December 12, 2006 - v2
Checksum:i49F2231445645B50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03025367 Genomic DNA. No translation available.
AF361756 mRNA. Translation: AAL91351.1.
RefSeqiNP_604458.1. NM_134463.1.
UniGeneiRn.3685.

Genome annotation databases

GeneIDi171497.
KEGGirno:171497.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03025367 Genomic DNA. No translation available.
AF361756 mRNA. Translation: AAL91351.1.
RefSeqiNP_604458.1. NM_134463.1.
UniGeneiRn.3685.

3D structure databases

ProteinModelPortaliQ8R4U9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040718.

PTM databases

iPTMnetiQ8R4U9.
PhosphoSitePlusiQ8R4U9.

Proteomic databases

PaxDbiQ8R4U9.
PRIDEiQ8R4U9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171497.
KEGGirno:171497.

Organism-specific databases

CTDi10110.
RGDi620232. Sgk2.

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
HOGENOMiHOG000233033.
HOVERGENiHBG108317.
InParanoidiQ8R4U9.
KOiK13303.
PhylomeDBiQ8R4U9.
TreeFamiTF320906.

Miscellaneous databases

PROiQ8R4U9.

Gene expression databases

BgeeiENSRNOG00000033573.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSGK2_RAT
AccessioniPrimary (citable) accession number: Q8R4U9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.