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Q8R4U6

- TOP1M_MOUSE

UniProt

Q8R4U6 - TOP1M_MOUSE

Protein

DNA topoisomerase I, mitochondrial

Gene

Top1mt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

    Cofactori

    Divalent metal ions (calcium or magnesium).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei145 – 1451Interaction with DNABy similarity
    Sitei193 – 1931Interaction with DNABy similarity
    Sitei241 – 2411Interaction with DNABy similarity
    Sitei272 – 2721Interaction with DNABy similarity
    Sitei330 – 3301Interaction with DNABy similarity
    Sitei361 – 3611Interaction with DNABy similarity
    Sitei403 – 4031Interaction with DNABy similarity
    Sitei461 – 4611Interaction with DNABy similarity
    Sitei479 – 4791Interaction with DNABy similarity
    Active sitei551 – 5511O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: RefGenome
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: RefGenome
    2. DNA topological change Source: RefGenome

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase I, mitochondrial (EC:5.99.1.2)
    Short name:
    TOP1mt
    Gene namesi
    Name:Top1mt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1920210. Top1mt.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. mitochondrial nucleoid Source: RefGenome
    3. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4343MitochondrionBy similarityAdd
    BLAST
    Chaini44 – 593550DNA topoisomerase I, mitochondrialPRO_0000384393Add
    BLAST

    Proteomic databases

    MaxQBiQ8R4U6.
    PaxDbiQ8R4U6.
    PRIDEiQ8R4U6.

    PTM databases

    PhosphoSiteiQ8R4U6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8R4U6.
    BgeeiQ8R4U6.
    GenevestigatoriQ8R4U6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8R4U6.
    SMRiQ8R4U6. Positions 37-593.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni254 – 2552Interaction with DNABy similarity
    Regioni317 – 3226Interaction with DNABy similarity
    Regioni414 – 4163Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3569.
    GeneTreeiENSGT00390000016347.
    HOGENOMiHOG000105469.
    InParanoidiQ8R4U6.
    KOiK03163.
    OMAiVINCSRD.
    OrthoDBiEOG7CVPX5.
    PhylomeDBiQ8R4U6.
    TreeFamiTF105281.

    Family and domain databases

    Gene3Di1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view]
    PfamiPF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SMARTiSM00435. TOPEUc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8R4U6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLLWLRALC RRFQHVPCRV PSCQVSRVAK ANRAGWEETS KSSVKWRQLE    50
    HKGPYFTPAY EPLPDGVHFL YDGKPLKLSL AAEEVATFYG KMLHLECTSK 100
    EVFRRNFFSD WRKEMTPEER RLITHLDKCD FTEIHKHFLE RAEARRTLPR 150
    EQKQKLKEEA EKLQQEFGYC VLDGHREKIG NFKTEPPGLF RGRGDHPKMG 200
    MLKRRVMPED VVINCSRDSK IPEPPAGHQW KEVRSDNTVM WLAAWVEHIQ 250
    NSFKYVILNP SSKPKGEMDW QKYEVARRLK GVVDKIRAQY QADWKSPEMK 300
    KRQLAVALYF IDKLALRTGN EKEEGETADT VGCCSLRVEH IRLHAPAGGQ 350
    EHVVELDFLG KDSIRYKNHV TVEKLVFQNL QHFMEDKDPR DDLFDALTTS 400
    SLNKHLQDLM EGLTAKVFRT YNASVTLQEQ LRVLTRAEDS VTCKVLAYNR 450
    ANRAVAVLCN HQRAVPKTFE KSMQTLQKKI ETKKAQVAEA QVELQKAETD 500
    LRMRGDSKPK SFLQKQRRLL KLEEQLARLC AKATDKEENK QVALGTAKLN 550
    YLDPRISIAW CKRFGVPVEK IYNKTQRERF AWAFNQAGED FEF 593
    Length:593
    Mass (Da):69,003
    Last modified:June 1, 2002 - v1
    Checksum:i7A934604BB586A32
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF362952 mRNA. Translation: AAM00270.1.
    AK147119 mRNA. Translation: BAE27691.1.
    CH466545 Genomic DNA. Translation: EDL29483.1.
    BC138582 mRNA. Translation: AAI38583.1.
    BC138583 mRNA. Translation: AAI38584.1.
    CCDSiCCDS27547.1.
    RefSeqiNP_082680.1. NM_028404.2.
    UniGeneiMm.182401.

    Genome annotation databases

    EnsembliENSMUST00000000958; ENSMUSP00000000958; ENSMUSG00000000934.
    GeneIDi72960.
    KEGGimmu:72960.
    UCSCiuc007whb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF362952 mRNA. Translation: AAM00270.1 .
    AK147119 mRNA. Translation: BAE27691.1 .
    CH466545 Genomic DNA. Translation: EDL29483.1 .
    BC138582 mRNA. Translation: AAI38583.1 .
    BC138583 mRNA. Translation: AAI38584.1 .
    CCDSi CCDS27547.1.
    RefSeqi NP_082680.1. NM_028404.2.
    UniGenei Mm.182401.

    3D structure databases

    ProteinModelPortali Q8R4U6.
    SMRi Q8R4U6. Positions 37-593.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8R4U6.

    Proteomic databases

    MaxQBi Q8R4U6.
    PaxDbi Q8R4U6.
    PRIDEi Q8R4U6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000958 ; ENSMUSP00000000958 ; ENSMUSG00000000934 .
    GeneIDi 72960.
    KEGGi mmu:72960.
    UCSCi uc007whb.1. mouse.

    Organism-specific databases

    CTDi 116447.
    MGIi MGI:1920210. Top1mt.

    Phylogenomic databases

    eggNOGi COG3569.
    GeneTreei ENSGT00390000016347.
    HOGENOMi HOG000105469.
    InParanoidi Q8R4U6.
    KOi K03163.
    OMAi VINCSRD.
    OrthoDBi EOG7CVPX5.
    PhylomeDBi Q8R4U6.
    TreeFami TF105281.

    Miscellaneous databases

    NextBioi 337229.
    PROi Q8R4U6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8R4U6.
    Bgeei Q8R4U6.
    Genevestigatori Q8R4U6.

    Family and domain databases

    Gene3Di 1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view ]
    Pfami PF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SMARTi SM00435. TOPEUc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Thirteen-exon-motif signature for vertebrate nuclear and mitochondrial type IB topoisomerases."
      Zhang H., Meng L.-H., Zimonjic D.B., Popescu N.C., Pommier Y.
      Nucleic Acids Res. 32:2087-2092(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X DBA/2.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiTOP1M_MOUSE
    AccessioniPrimary (citable) accession number: Q8R4U6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3