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Q8R4U6

- TOP1M_MOUSE

UniProt

Q8R4U6 - TOP1M_MOUSE

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Protein

DNA topoisomerase I, mitochondrial

Gene

Top1mt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactori

Divalent metal ions (calcium or magnesium).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei145 – 1451Interaction with DNABy similarity
Sitei193 – 1931Interaction with DNABy similarity
Sitei241 – 2411Interaction with DNABy similarity
Sitei272 – 2721Interaction with DNABy similarity
Sitei330 – 3301Interaction with DNABy similarity
Sitei361 – 3611Interaction with DNABy similarity
Sitei403 – 4031Interaction with DNABy similarity
Sitei461 – 4611Interaction with DNABy similarity
Sitei479 – 4791Interaction with DNABy similarity
Active sitei551 – 5511O-(3'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: RefGenome
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: RefGenome
  2. DNA topological change Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase I, mitochondrial (EC:5.99.1.2)
Short name:
TOP1mt
Gene namesi
Name:Top1mt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1920210. Top1mt.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. mitochondrial nucleoid Source: RefGenome
  3. mitochondrion Source: MGI
  4. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4343MitochondrionBy similarityAdd
BLAST
Chaini44 – 593550DNA topoisomerase I, mitochondrialPRO_0000384393Add
BLAST

Proteomic databases

MaxQBiQ8R4U6.
PaxDbiQ8R4U6.
PRIDEiQ8R4U6.

PTM databases

PhosphoSiteiQ8R4U6.

Expressioni

Gene expression databases

BgeeiQ8R4U6.
ExpressionAtlasiQ8R4U6. baseline and differential.
GenevestigatoriQ8R4U6.

Structurei

3D structure databases

ProteinModelPortaliQ8R4U6.
SMRiQ8R4U6. Positions 37-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 2552Interaction with DNABy similarity
Regioni317 – 3226Interaction with DNABy similarity
Regioni414 – 4163Interaction with DNABy similarity

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3569.
GeneTreeiENSGT00390000016347.
HOGENOMiHOG000105469.
InParanoidiQ8R4U6.
KOiK03163.
OMAiVINCSRD.
OrthoDBiEOG7CVPX5.
PhylomeDBiQ8R4U6.
TreeFamiTF105281.

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8R4U6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLLWLRALC RRFQHVPCRV PSCQVSRVAK ANRAGWEETS KSSVKWRQLE
60 70 80 90 100
HKGPYFTPAY EPLPDGVHFL YDGKPLKLSL AAEEVATFYG KMLHLECTSK
110 120 130 140 150
EVFRRNFFSD WRKEMTPEER RLITHLDKCD FTEIHKHFLE RAEARRTLPR
160 170 180 190 200
EQKQKLKEEA EKLQQEFGYC VLDGHREKIG NFKTEPPGLF RGRGDHPKMG
210 220 230 240 250
MLKRRVMPED VVINCSRDSK IPEPPAGHQW KEVRSDNTVM WLAAWVEHIQ
260 270 280 290 300
NSFKYVILNP SSKPKGEMDW QKYEVARRLK GVVDKIRAQY QADWKSPEMK
310 320 330 340 350
KRQLAVALYF IDKLALRTGN EKEEGETADT VGCCSLRVEH IRLHAPAGGQ
360 370 380 390 400
EHVVELDFLG KDSIRYKNHV TVEKLVFQNL QHFMEDKDPR DDLFDALTTS
410 420 430 440 450
SLNKHLQDLM EGLTAKVFRT YNASVTLQEQ LRVLTRAEDS VTCKVLAYNR
460 470 480 490 500
ANRAVAVLCN HQRAVPKTFE KSMQTLQKKI ETKKAQVAEA QVELQKAETD
510 520 530 540 550
LRMRGDSKPK SFLQKQRRLL KLEEQLARLC AKATDKEENK QVALGTAKLN
560 570 580 590
YLDPRISIAW CKRFGVPVEK IYNKTQRERF AWAFNQAGED FEF
Length:593
Mass (Da):69,003
Last modified:June 1, 2002 - v1
Checksum:i7A934604BB586A32
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF362952 mRNA. Translation: AAM00270.1.
AK147119 mRNA. Translation: BAE27691.1.
CH466545 Genomic DNA. Translation: EDL29483.1.
BC138582 mRNA. Translation: AAI38583.1.
BC138583 mRNA. Translation: AAI38584.1.
CCDSiCCDS27547.1.
RefSeqiNP_082680.1. NM_028404.2.
UniGeneiMm.182401.

Genome annotation databases

EnsembliENSMUST00000000958; ENSMUSP00000000958; ENSMUSG00000000934.
GeneIDi72960.
KEGGimmu:72960.
UCSCiuc007whb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF362952 mRNA. Translation: AAM00270.1 .
AK147119 mRNA. Translation: BAE27691.1 .
CH466545 Genomic DNA. Translation: EDL29483.1 .
BC138582 mRNA. Translation: AAI38583.1 .
BC138583 mRNA. Translation: AAI38584.1 .
CCDSi CCDS27547.1.
RefSeqi NP_082680.1. NM_028404.2.
UniGenei Mm.182401.

3D structure databases

ProteinModelPortali Q8R4U6.
SMRi Q8R4U6. Positions 37-593.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8R4U6.

Proteomic databases

MaxQBi Q8R4U6.
PaxDbi Q8R4U6.
PRIDEi Q8R4U6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000958 ; ENSMUSP00000000958 ; ENSMUSG00000000934 .
GeneIDi 72960.
KEGGi mmu:72960.
UCSCi uc007whb.1. mouse.

Organism-specific databases

CTDi 116447.
MGIi MGI:1920210. Top1mt.

Phylogenomic databases

eggNOGi COG3569.
GeneTreei ENSGT00390000016347.
HOGENOMi HOG000105469.
InParanoidi Q8R4U6.
KOi K03163.
OMAi VINCSRD.
OrthoDBi EOG7CVPX5.
PhylomeDBi Q8R4U6.
TreeFami TF105281.

Miscellaneous databases

NextBioi 337229.
PROi Q8R4U6.
SOURCEi Search...

Gene expression databases

Bgeei Q8R4U6.
ExpressionAtlasi Q8R4U6. baseline and differential.
Genevestigatori Q8R4U6.

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Thirteen-exon-motif signature for vertebrate nuclear and mitochondrial type IB topoisomerases."
    Zhang H., Meng L.-H., Zimonjic D.B., Popescu N.C., Pommier Y.
    Nucleic Acids Res. 32:2087-2092(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X DBA/2.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiTOP1M_MOUSE
AccessioniPrimary (citable) accession number: Q8R4U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3