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Q8R4U6 (TOP1M_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase I, mitochondrial

Short name=TOP1mt
EC=5.99.1.2
Gene names
Name:Top1mt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactor

Divalent metal ions (calcium or magnesium) By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion By similarity
Chain44 – 593550DNA topoisomerase I, mitochondrial
PRO_0000384393

Regions

Region254 – 2552Interaction with DNA By similarity
Region317 – 3226Interaction with DNA By similarity
Region414 – 4163Interaction with DNA By similarity

Sites

Active site5511O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Site1451Interaction with DNA By similarity
Site1931Interaction with DNA By similarity
Site2411Interaction with DNA By similarity
Site2721Interaction with DNA By similarity
Site3301Interaction with DNA By similarity
Site3611Interaction with DNA By similarity
Site4031Interaction with DNA By similarity
Site4611Interaction with DNA By similarity
Site4791Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8R4U6 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 7A934604BB586A32

FASTA59369,003
        10         20         30         40         50         60 
MLLLWLRALC RRFQHVPCRV PSCQVSRVAK ANRAGWEETS KSSVKWRQLE HKGPYFTPAY 

        70         80         90        100        110        120 
EPLPDGVHFL YDGKPLKLSL AAEEVATFYG KMLHLECTSK EVFRRNFFSD WRKEMTPEER 

       130        140        150        160        170        180 
RLITHLDKCD FTEIHKHFLE RAEARRTLPR EQKQKLKEEA EKLQQEFGYC VLDGHREKIG 

       190        200        210        220        230        240 
NFKTEPPGLF RGRGDHPKMG MLKRRVMPED VVINCSRDSK IPEPPAGHQW KEVRSDNTVM 

       250        260        270        280        290        300 
WLAAWVEHIQ NSFKYVILNP SSKPKGEMDW QKYEVARRLK GVVDKIRAQY QADWKSPEMK 

       310        320        330        340        350        360 
KRQLAVALYF IDKLALRTGN EKEEGETADT VGCCSLRVEH IRLHAPAGGQ EHVVELDFLG 

       370        380        390        400        410        420 
KDSIRYKNHV TVEKLVFQNL QHFMEDKDPR DDLFDALTTS SLNKHLQDLM EGLTAKVFRT 

       430        440        450        460        470        480 
YNASVTLQEQ LRVLTRAEDS VTCKVLAYNR ANRAVAVLCN HQRAVPKTFE KSMQTLQKKI 

       490        500        510        520        530        540 
ETKKAQVAEA QVELQKAETD LRMRGDSKPK SFLQKQRRLL KLEEQLARLC AKATDKEENK 

       550        560        570        580        590 
QVALGTAKLN YLDPRISIAW CKRFGVPVEK IYNKTQRERF AWAFNQAGED FEF 

« Hide

References

« Hide 'large scale' references
[1]"Thirteen-exon-motif signature for vertebrate nuclear and mitochondrial type IB topoisomerases."
Zhang H., Meng L.-H., Zimonjic D.B., Popescu N.C., Pommier Y.
Nucleic Acids Res. 32:2087-2092(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X DBA/2.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF362952 mRNA. Translation: AAM00270.1.
AK147119 mRNA. Translation: BAE27691.1.
CH466545 Genomic DNA. Translation: EDL29483.1.
BC138582 mRNA. Translation: AAI38583.1.
BC138583 mRNA. Translation: AAI38584.1.
CCDSCCDS27547.1.
RefSeqNP_082680.1. NM_028404.2.
UniGeneMm.182401.

3D structure databases

ProteinModelPortalQ8R4U6.
SMRQ8R4U6. Positions 37-593.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8R4U6.

Proteomic databases

MaxQBQ8R4U6.
PaxDbQ8R4U6.
PRIDEQ8R4U6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000958; ENSMUSP00000000958; ENSMUSG00000000934.
GeneID72960.
KEGGmmu:72960.
UCSCuc007whb.1. mouse.

Organism-specific databases

CTD116447.
MGIMGI:1920210. Top1mt.

Phylogenomic databases

eggNOGCOG3569.
GeneTreeENSGT00390000016347.
HOGENOMHOG000105469.
InParanoidQ8R4U6.
KOK03163.
OMAVINCSRD.
OrthoDBEOG7CVPX5.
PhylomeDBQ8R4U6.
TreeFamTF105281.

Gene expression databases

ArrayExpressQ8R4U6.
BgeeQ8R4U6.
GenevestigatorQ8R4U6.

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
ProtoNetSearch...

Other

NextBio337229.
PROQ8R4U6.
SOURCESearch...

Entry information

Entry nameTOP1M_MOUSE
AccessionPrimary (citable) accession number: Q8R4U6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot